Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_K23
(889 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.29
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.67
SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 29 0.88
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 29 1.2
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 2.0
SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 26 6.2
SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizo... 26 8.2
SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 26 8.2
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 8.2
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 26 8.2
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.7 bits (66), Expect = 0.29
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 673
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVP 360
Query: 674 SNTTGT 691
+T +
Sbjct: 361 PTSTSS 366
Score = 30.7 bits (66), Expect = 0.29
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 673
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVP 414
Query: 674 SNTTGT 691
++ T
Sbjct: 415 PTSSST 420
Score = 29.9 bits (64), Expect = 0.51
Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +2
Query: 503 PKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSN 679
P T + S P P T+ TS S PP NST + +T V + SST P P++
Sbjct: 264 PPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNST-TPVTPTVPPTSTSSTSTPP-PPAS 321
Query: 680 TTGT 691
T+ T
Sbjct: 322 TSST 325
Score = 29.5 bits (63), Expect = 0.67
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 673
+TP P S S+P P T+ + S P T ST + + + S+T + P P
Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306
Query: 674 SNTTGT 691
+T +
Sbjct: 307 PTSTSS 312
Score = 29.1 bits (62), Expect = 0.88
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +2
Query: 497 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 673
+TP P S S+P P T+ + S P T ST S T + SS++ P+TP
Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249
Query: 674 S 676
+
Sbjct: 250 T 250
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = +2
Query: 488 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP 664
L++ TP T P S S W TT T S T+ST +T T ++ +
Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657
Query: 665 LTPSNTTGT 691
T S++T T
Sbjct: 658 STSSSSTAT 666
>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 29.5 bits (63), Expect = 0.67
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -2
Query: 579 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 478
DLE N +V Q+ G G+FL G FG + FV+
Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444
>SPCC576.13 |swc5||chromatin remodeling complex subunit
Swc5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 215
Score = 29.1 bits (62), Expect = 0.88
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +1
Query: 223 ETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKE 366
ET K S K +K + + +++++ K NT++ A Q W+K KE
Sbjct: 128 ETPKKKHSLIRKRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKE 175
>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -2
Query: 711 FVHGGLKVPVVFEGVSGAITVDDTVIT 631
F H + V G+ GAIT+DD++IT
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIIT 133
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 190 QLYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 306
Q+++++V+ Y A+ EYL+EK I + K ++E
Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595
>SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 892
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 6/51 (11%)
Frame = +1
Query: 631 SDDRIIYGDSTADTFKHHWYLEP----SMYESDVMFFVYNR--EYNSVMTL 765
SDDR+ GD+ + +Y++ +MYE ++ Y YN V+ L
Sbjct: 84 SDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLAL 134
>SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase
Pik3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 801
Score = 25.8 bits (54), Expect = 8.2
Identities = 34/111 (30%), Positives = 49/111 (44%), Gaps = 7/111 (6%)
Frame = +1
Query: 370 VKSYFPIQFRVIFTEQTVKLINKXDHHALKL----IDQQNH-NKIAFGDSKDKTSKKVSW 534
+K Y P + + VK IN+ D LKL + N + I+FG KD SK +S
Sbjct: 139 IKKYSPTSLEL----EQVKEINRLDGLLLKLQLGDVPSVNWLDDISFGKIKDFRSKHMSL 194
Query: 535 KFTPVLENNRVYFKIMSTEDKQYL-KLDN-TKGSSDDRIIYGDSTADTFKH 681
P+L + + F + Y K +N SS D + DS A+ KH
Sbjct: 195 VTIPILYLDFLQFSFPVVFQRSYYPKSENRVYYSSFDLELNLDSPAE-LKH 244
>SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 25.8 bits (54), Expect = 8.2
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = -3
Query: 326 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVAL 147
M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++
Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57
Query: 146 EASAHTARTKA 114
+A A T A
Sbjct: 58 NGAAKEAATAA 68
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 25.8 bits (54), Expect = 8.2
Identities = 22/82 (26%), Positives = 40/82 (48%)
Frame = -3
Query: 293 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 114
F S ++ F R+S + S TT+ + S SVL ++ A+E S TA ++
Sbjct: 3567 FVPSSVSRSFSYSRFSSGSLDSSSVFNSTTVSTASGISQGSVLSSTRAIE-SESTASHRS 3625
Query: 113 NKVSLILAQWLSLKASQQTTSK 48
+ +S + + LS A ++S+
Sbjct: 3626 SVLSELSSYDLSTSAFSSSSSE 3647
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 4/49 (8%)
Frame = +1
Query: 685 WYLEPSMYE--SDVMFFVYNREYNSVMTLDEXMAANE--XREXWGTAEK 819
+Y EP+ + D + YNR Y V++LD+ + +E + W TA++
Sbjct: 155 YYQEPAFEKLLPDPLPEPYNRPYTLVLSLDDLLIHSEWTRQHGWRTAKR 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,529,048
Number of Sequences: 5004
Number of extensions: 72670
Number of successful extensions: 288
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 254
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 286
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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