Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_I22
(866 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein. 128 9e-32
EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein. 127 2e-31
EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein. 123 2e-30
EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein. 123 2e-30
EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein. 113 2e-27
AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein. 113 2e-27
EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage prot... 93 2e-21
AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase prot... 62 6e-12
DQ325083-1|ABD14097.1| 189|Apis mellifera complementary sex det... 23 2.8
AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein. 23 2.8
AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 22 6.4
AY350618-1|AAQ57660.1| 425|Apis mellifera complementary sex det... 22 8.4
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 22 8.4
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 22 8.4
>EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein.
Length = 686
Score = 128 bits (308), Expect = 9e-32
Identities = 67/159 (42%), Positives = 91/159 (57%), Gaps = 2/159 (1%)
Frame = +2
Query: 395 EAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVVPAPYEVY 574
E LF L Y AKDF+TFYKTA +AR+ +N G F AF IAV+ R D PA YE+Y
Sbjct: 107 EVRMLFELLYNAKDFQTFYKTAAWARLRMNSGMFTTAFSIAVLYRPDTKYMKFPAIYEIY 166
Query: 575 PKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVL--YNNEEQR 748
P F + V+++ KM G ++ G++ Y V NYS+ + YN+ E +
Sbjct: 167 PNYFFDSSVIEEAQNLKMSRG-----SSVVTGMNNIETYIV-NTNYSSKYMREYNDPEYK 220
Query: 749 LTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRGE 865
L YF ED+ +NAYYYY LP+W +S +Y KE RG+
Sbjct: 221 LDYFMEDVELNAYYYYMREMLPYWMSSSQYHMPKEIRGQ 259
Score = 58.0 bits (134), Expect = 1e-10
Identities = 29/70 (41%), Positives = 43/70 (61%), Gaps = 2/70 (2%)
Frame = +1
Query: 166 DAVFVEKQKKILSFFQDVSQLNTDD-EYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTG 342
D F+ KQKKI V Q + D E+Y +G++YD+E NMD Y +K V++FL Y+ G
Sbjct: 29 DMDFLHKQKKIFDLLLYVRQADLSDAEWYDVGRNYDMESNMDMYKDKNVVQKFLWWYKQG 88
Query: 343 -FMPKNLEFS 369
F+ +N F+
Sbjct: 89 MFLSRNAIFT 98
>EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein.
Length = 686
Score = 127 bits (306), Expect = 2e-31
Identities = 68/159 (42%), Positives = 90/159 (56%), Gaps = 2/159 (1%)
Frame = +2
Query: 395 EAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVVPAPYEVY 574
E LF L Y AKDF+TFYKTA +AR+ +N G F AF IAV+ R D PA YE+Y
Sbjct: 107 EVRMLFELLYNAKDFQTFYKTAAWARLRMNSGMFTTAFSIAVLYRPDTKYMKFPAIYEIY 166
Query: 575 PKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYS--NAVLYNNEEQR 748
P F + V+++ KM G ++ G++ Y V NYS N YN+ E +
Sbjct: 167 PNYFFDSSVIEEAQNLKMSRG-----SSVVTGMNNIETYIV-NTNYSSKNMREYNDPEYK 220
Query: 749 LTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRGE 865
L YF ED+ +NAYYYY LP+W +S +Y KE RG+
Sbjct: 221 LDYFMEDVELNAYYYYMREMLPYWMSSSQYHMPKEIRGQ 259
Score = 58.0 bits (134), Expect = 1e-10
Identities = 29/70 (41%), Positives = 43/70 (61%), Gaps = 2/70 (2%)
Frame = +1
Query: 166 DAVFVEKQKKILSFFQDVSQLNTDD-EYYKIGKDYDIEMNMDNYTNKKAVEEFLKMYRTG 342
D F+ KQKKI V Q + D E+Y +G++YD+E NMD Y +K V++FL Y+ G
Sbjct: 29 DMDFLHKQKKIFDLLLYVRQADLSDAEWYDVGRNYDMESNMDMYKDKNVVQKFLWWYKQG 88
Query: 343 -FMPKNLEFS 369
F+ +N F+
Sbjct: 89 MFLSRNAIFT 98
>EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein.
Length = 684
Score = 123 bits (297), Expect = 2e-30
Identities = 68/166 (40%), Positives = 99/166 (59%), Gaps = 2/166 (1%)
Frame = +2
Query: 374 FYDKMRDEAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVV 553
+Y ++ E ALF LFY+AKDF+ F+KTA +A+ ++N+ Q++Y+ Y AVI R D +
Sbjct: 100 YYPQLLREMSALFKLFYHAKDFDIFFKTALWAKNNINEAQYIYSLYTAVITRPDTKFIQL 159
Query: 554 PAPYEVYPKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVLYN 733
P YE+ P F N EVLQK + G ++ + + KY KE ++ ANYS L +
Sbjct: 160 PPLYEMCPYFFFNSEVLQKAN-HALIFGKLDTKTSGKY---KE---YIIPANYSGWYLNH 212
Query: 734 --NEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRGE 865
N E +L YF EDIG+N YY++ PFW S++Y L + RGE
Sbjct: 213 DYNLENKLNYFIEDIGLNTYYFFLRQAFPFWLPSKEYD-LPDYRGE 257
Score = 58.4 bits (135), Expect = 8e-11
Identities = 29/75 (38%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = +1
Query: 154 TKNVDAVFVEKQKKILSFFQDVSQ-LNTDDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKM 330
TK D F+ KQKK+ + V+Q + +Y G+ ++IE N+D+YTN AV+EFL +
Sbjct: 26 TKTADKDFLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIEANIDSYTNAAAVKEFLSI 85
Query: 331 YRTGFMPKNLEFSXF 375
Y+ G +P+ FS +
Sbjct: 86 YKHGMLPRGELFSLY 100
>EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein.
Length = 684
Score = 123 bits (296), Expect = 2e-30
Identities = 68/166 (40%), Positives = 99/166 (59%), Gaps = 2/166 (1%)
Frame = +2
Query: 374 FYDKMRDEAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVV 553
+Y ++ E ALF LFY+AKDF+ F+KTA +A+ ++N+ Q++Y+ Y AVI R D +
Sbjct: 100 YYPQLLREMSALFKLFYHAKDFDIFFKTALWAKNNINEAQYIYSLYTAVITRPDTKFIQL 159
Query: 554 PAPYEVYPKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVLYN 733
P YE+ P F N EVLQK + G ++ + + KY KE ++ ANYS L +
Sbjct: 160 PPLYEMCPYFFFNSEVLQKAN-HALIFGKLDTKTSGKY---KE---YIIPANYSGWYLNH 212
Query: 734 --NEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRGE 865
N E +L YF EDIG+N YY++ PFW S++Y L + RGE
Sbjct: 213 DYNLENKLIYFIEDIGLNTYYFFLRQAFPFWLPSKEYD-LPDYRGE 257
Score = 58.4 bits (135), Expect = 8e-11
Identities = 29/75 (38%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = +1
Query: 154 TKNVDAVFVEKQKKILSFFQDVSQ-LNTDDEYYKIGKDYDIEMNMDNYTNKKAVEEFLKM 330
TK D F+ KQKK+ + V+Q + +Y G+ ++IE N+D+YTN AV+EFL +
Sbjct: 26 TKTADKDFLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIEANIDSYTNAAAVKEFLSI 85
Query: 331 YRTGFMPKNLEFSXF 375
Y+ G +P+ FS +
Sbjct: 86 YKHGMLPRGELFSLY 100
>EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein.
Length = 683
Score = 113 bits (273), Expect = 2e-27
Identities = 61/148 (41%), Positives = 86/148 (58%), Gaps = 3/148 (2%)
Frame = +2
Query: 383 KMRDEAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVVPAP 562
+MR +A+ LF L Y AK F+ FY TA +AR ++N+ +LYA +AVI R D +P
Sbjct: 101 EMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVNEQMYLYALSVAVIHRPDTKLMKLPPM 160
Query: 563 YEVYPKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVL--YNN 736
YEV P ++ N EV+QK Y M D A + DY++ ANY+ L +N
Sbjct: 161 YEVMPHLYFNDEVMQKAYNIAMGD------TADMKKTYNNIDYYLLAANYTGWYLTKHNV 214
Query: 737 EEQRLTYFTEDIGMNAYYYYF-HSHLPF 817
EQRL YFTED+G+N +Y+ H++ PF
Sbjct: 215 PEQRLNYFTEDVGLNHFYFMLNHNYPPF 242
Score = 50.4 bits (115), Expect = 2e-08
Identities = 23/75 (30%), Positives = 41/75 (54%), Gaps = 1/75 (1%)
Frame = +1
Query: 148 IKTKNVDAVFVEKQKKILSFFQDVSQLNT-DDEYYKIGKDYDIEMNMDNYTNKKAVEEFL 324
+ K D +V +QK I F V Q E Y+ + +++ N+DNY +K+AV EF+
Sbjct: 22 VPNKVADKTYVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFM 81
Query: 325 KMYRTGFMPKNLEFS 369
++ + G +P+ F+
Sbjct: 82 QLLKHGMLPRGQVFT 96
>AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein.
Length = 683
Score = 113 bits (273), Expect = 2e-27
Identities = 61/148 (41%), Positives = 86/148 (58%), Gaps = 3/148 (2%)
Frame = +2
Query: 383 KMRDEAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVVPAP 562
+MR +A+ LF L Y AK F+ FY TA +AR ++N+ +LYA +AVI R D +P
Sbjct: 101 EMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVNEQMYLYALSVAVIHRPDTKLMKLPPM 160
Query: 563 YEVYPKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVL--YNN 736
YEV P ++ N EV+QK Y M D A + DY++ ANY+ L +N
Sbjct: 161 YEVMPHLYFNDEVMQKAYNIAMGD------TADMKKTYNNIDYYLLAANYTGWYLTKHNV 214
Query: 737 EEQRLTYFTEDIGMNAYYYYF-HSHLPF 817
EQRL YFTED+G+N +Y+ H++ PF
Sbjct: 215 PEQRLNYFTEDVGLNHFYFMLNHNYPPF 242
Score = 50.4 bits (115), Expect = 2e-08
Identities = 23/75 (30%), Positives = 41/75 (54%), Gaps = 1/75 (1%)
Frame = +1
Query: 148 IKTKNVDAVFVEKQKKILSFFQDVSQLNT-DDEYYKIGKDYDIEMNMDNYTNKKAVEEFL 324
+ K D +V +QK I F V Q E Y+ + +++ N+DNY +K+AV EF+
Sbjct: 22 VPNKVADKTYVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFM 81
Query: 325 KMYRTGFMPKNLEFS 369
++ + G +P+ F+
Sbjct: 82 QLLKHGMLPRGQVFT 96
>EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage protein
protein.
Length = 1010
Score = 93.5 bits (222), Expect = 2e-21
Identities = 50/136 (36%), Positives = 80/136 (58%)
Frame = +2
Query: 383 KMRDEAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVVPAP 562
++R E L+ + AKD++TF KTA +ARVH+N+GQFL AF AV+ R D + P
Sbjct: 99 QLRKEVSLLYRILLGAKDYQTFLKTAAWARVHVNEGQFLKAFVAAVLTRQDTQSVIFPPV 158
Query: 563 YEVYPKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVLYNNEE 742
YE+ P+ ++ V+Q+ +Q+ G + + + + NYS L +++E
Sbjct: 159 YEILPQHHLDSRVIQEAQNIAIQN---------TQGKNNQQNILI-PVNYS--ALLSHDE 206
Query: 743 QRLTYFTEDIGMNAYY 790
Q+L+YFT+DIG+ AYY
Sbjct: 207 QQLSYFTQDIGLAAYY 222
Score = 39.9 bits (89), Expect = 3e-05
Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Frame = +1
Query: 148 IKTKNVDAVFVEKQKKILSFFQDVSQLNTDDEYYKIGKDYDIEMNMDNYTNKKAVEEFLK 327
+K + D + KQ+ ++ Q +SQ + E +G YDIE N Y N V +
Sbjct: 20 VKQRAADQDLLNKQQDVIQLLQKISQPIPNQELQNLGASYDIESNSHQYKNPIIVMYYAG 79
Query: 328 MYRTGFM-PKNLEFS 369
+ G + P+ FS
Sbjct: 80 AVKAGLVQPQGTTFS 94
>AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase
protein.
Length = 693
Score = 62.1 bits (144), Expect = 6e-12
Identities = 46/164 (28%), Positives = 73/164 (44%)
Frame = +2
Query: 374 FYDKMRDEAIALFHLFYYAKDFETFYKTACFARVHLNQGQFLYAFYIAVIQRSDCHGFVV 553
F R A L +F + +E F A + R LN F+YA +A++ R D V
Sbjct: 85 FIPAHRKIAARLIDIFMGMRTYEDFLSVAVYCRDRLNPNLFIYALSVAILHRPDTKDLPV 144
Query: 554 PAPYEVYPKMFMNMEVLQKIYVTKMQDGLINPEAAAKYGIHKENDYFVYKANYSNAVLYN 733
P EV+P +M+ I+ ++ + PE A+ I DY +
Sbjct: 145 PPLTEVFPDKYMD----SGIFSRAREEANVVPE-GARVPIEIPRDYTASDLDV------- 192
Query: 734 NEEQRLTYFTEDIGMNAYYYYFHSHLPFWWTSEKYGALKERRGE 865
E R+ Y+ EDIG+N +++++H PF + K+RRGE
Sbjct: 193 --EHRVAYWREDIGINLHHWHWHLVYPF--EGDIRIVNKDRRGE 232
>DQ325083-1|ABD14097.1| 189|Apis mellifera complementary sex
determiner protein.
Length = 189
Score = 23.4 bits (48), Expect = 2.8
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +2
Query: 671 IHKENDYFVYKANYSNAVLYNN 736
IH N+Y K NY+N YNN
Sbjct: 90 IHNNNNY---KYNYNNKYNYNN 108
>AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein.
Length = 429
Score = 23.4 bits (48), Expect = 2.8
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +3
Query: 60 SSAGNHEVCLNSGWACSRRAQQCSTKAEHHKDKK 161
++AGN+E +SG A S R ++ EH + +
Sbjct: 254 NAAGNNEDSSDSGAAASDRPPASASSNEHEAESE 287
>AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha-3 protein.
Length = 537
Score = 22.2 bits (45), Expect = 6.4
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = -3
Query: 780 FMPISSVKYVSLCSSLL 730
++P S + VSLCSS+L
Sbjct: 272 YLPSDSGEKVSLCSSIL 288
>AY350618-1|AAQ57660.1| 425|Apis mellifera complementary sex
determiner protein.
Length = 425
Score = 21.8 bits (44), Expect = 8.4
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +2
Query: 674 HKENDYFVYKANYSNAVLYNN 736
+K ++Y Y NY+N YNN
Sbjct: 322 YKYSNYNNYNNNYNNYNNYNN 342
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 21.8 bits (44), Expect = 8.4
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +2
Query: 767 DIGMNAYYYYF 799
D GM YYY+F
Sbjct: 469 DRGMKVYYYFF 479
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 21.8 bits (44), Expect = 8.4
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +2
Query: 767 DIGMNAYYYYF 799
D GM YYY+F
Sbjct: 469 DRGMKVYYYFF 479
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 221,597
Number of Sequences: 438
Number of extensions: 4743
Number of successful extensions: 39
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 28038087
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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