Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_I08
(932 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.011
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.044
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.13
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.23
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.23
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.71
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 5.0
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.5 bits (78), Expect = 0.011
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = +1
Query: 631 PXPXKXPFGGXXPXSPPPPGXXPXKPPPP 717
P P P G P PPPPG PPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 34.3 bits (75), Expect = 0.025
Identities = 19/53 (35%), Positives = 20/53 (37%)
Frame = +2
Query: 437 KXPPPPGXXXXXXGPXPLXPPHXPXAPVFRGGGXXXXPPRGEXXFXPKPGXXP 595
K PPPP P P P P AP+ GG PP G P P P
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIM-GGPPPPPPPPGVAGAGPPPPPPP 781
Score = 33.9 bits (74), Expect = 0.033
Identities = 16/40 (40%), Positives = 16/40 (40%)
Frame = +1
Query: 679 PPPGXXPXKPPPPXXGGXPPXXL*XSPGFXPLXPPXXXPP 798
P P P PP P GG PP PG PP PP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPP--PPPGVAGAGPPPPPPP 781
Score = 32.7 bits (71), Expect = 0.076
Identities = 18/53 (33%), Positives = 19/53 (35%)
Frame = +3
Query: 435 KXPPPPRGXGXXFXGPXPXPPPIXPXPXFFGGGXXFXPPXGGKXXXPPNXGXP 593
K PPPP P P P P+ P GG PP G PP P
Sbjct: 730 KSPPPPP-PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.9 bits (59), Expect = 2.2
Identities = 16/51 (31%), Positives = 17/51 (33%)
Frame = +2
Query: 428 PXXKXPPPPGXXXXXXGPXPLXPPHXPXAPVFRGGGXXXXPPRGEXXFXPK 580
P PPPP G P PP P P GG P + PK
Sbjct: 756 PIMGGPPPPPPPPGVAGAGP--PPPPPPPPAVSAGGSRYYAPAPQAEPEPK 804
Score = 27.5 bits (58), Expect = 2.9
Identities = 13/32 (40%), Positives = 13/32 (40%), Gaps = 2/32 (6%)
Frame = +1
Query: 649 PFGGXXPXSPPPP--GXXPXKPPPPXXGGXPP 738
P P PP P G P PPPP G P
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP 775
Score = 25.8 bits (54), Expect = 8.7
Identities = 17/57 (29%), Positives = 18/57 (31%)
Frame = +1
Query: 547 PPXGGXXVXPQTXGXPXKKLXXQGAXXGPXPXKXPFGGXXPXSPPPPGXXPXKPPPP 717
PP + P P GP P P G PPPP PPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP------PPPP 783
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 33.5 bits (73), Expect = 0.044
Identities = 29/112 (25%), Positives = 32/112 (28%), Gaps = 1/112 (0%)
Frame = +1
Query: 481 PXPXPPP*XPXPXFSGGGXXXXPPXGGXXVXPQTXGXPXKKLXXQGAXXGPXPXKX-PFG 657
P P P P P S P G V + P G P P P
Sbjct: 1132 PVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPV 1191
Query: 658 GXXPXSPPPPGXXPXKPPPPXXGGXPPXXL*XSPGFXPLXPPXXXPPFFPXP 813
+PP P PP P PP S G P+ P P P P
Sbjct: 1192 PPPSEAPPVPKPSVGVPPVPPPSTAPPVPT-PSAGLPPVPVPTAKAPPVPAP 1242
Score = 31.5 bits (68), Expect = 0.18
Identities = 34/126 (26%), Positives = 39/126 (30%), Gaps = 2/126 (1%)
Frame = +1
Query: 442 PPPPGXXGFXFXAPXPXPP-P*XPXPXFSGGGXXXXPPXGGXXVXPQTXGXPXKKLXXQG 618
PP P G P P P P P S P G V + P + G
Sbjct: 1083 PPVPAPSGIP---PVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVP-SG 1138
Query: 619 AXXGPXPX-KXPFGGXXPXSPPPPGXXPXKPPPPXXGGXPPXXL*XSPGFXPLXPPXXXP 795
A P P P +PP P PP P P + G P+ PP P
Sbjct: 1139 APPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAP 1198
Query: 796 PFFPXP 813
P P P
Sbjct: 1199 P-VPKP 1203
Score = 27.5 bits (58), Expect = 2.9
Identities = 28/113 (24%), Positives = 31/113 (27%), Gaps = 2/113 (1%)
Frame = +3
Query: 480 PXPXPPPIXPXPXFFGGGXXFXPPXGGKXXXPPNXGXPXXK--TFXPRGPKXPXXXKGPX 653
P P P P P P G P+ P + P PK P P
Sbjct: 1113 PVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPK-PSVAAPPV 1171
Query: 654 WGXFXXXPPPPWXXSXKTXPPXXXGXPPXXXXKXPXXXPPXXPPXXPPFFPXP 812
PP P + P PP K PP PP P P P
Sbjct: 1172 PAPSSGIPPVPKPAAGVPPVPPPSEAPPVP--KPSVGVPPVPPPSTAPPVPTP 1222
Score = 27.5 bits (58), Expect = 2.9
Identities = 27/101 (26%), Positives = 29/101 (28%), Gaps = 2/101 (1%)
Frame = +1
Query: 442 PPPPGXXGFXFXAPXPXPP-P*XPXPXFSGGGXXXXPPXGGXXVXPQTXGXPXKKLXXQG 618
PP P G P P P P P SG P V + G P G
Sbjct: 1131 PPVPVPSG---APPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAG 1187
Query: 619 AXXGPXPXKXPFGGXXPXSPPPPGXXPXKPPPPXX-GGXPP 738
P P + P PP PP P G PP
Sbjct: 1188 VPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPP 1228
Score = 26.6 bits (56), Expect = 5.0
Identities = 29/107 (27%), Positives = 30/107 (28%), Gaps = 5/107 (4%)
Frame = +1
Query: 508 PXPXFSGGGXXXXPPXGGXXVXPQTXGXPXKKLXXQGAXXGPXPXKXPFGGXXPXSPPPP 687
P P S P V T P K GA P P P P P P
Sbjct: 1023 PVPLPSADAPPIPVPSTAPPVPIPTSTPPVPK-SSSGAPSAPPPVPAP-SSEIPSIPAPS 1080
Query: 688 GXXPXK-----PPPPXXGGXPPXXL*XSPGFXPLXPPXXXPPFFPXP 813
G P PP P P S P+ P PP P P
Sbjct: 1081 GAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPP-VPKP 1126
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.9 bits (69), Expect = 0.13
Identities = 34/134 (25%), Positives = 36/134 (26%), Gaps = 8/134 (5%)
Frame = +3
Query: 447 PPRGXGXXFXGPXPXPPPIXPXPXFFGGGXXFXPPXGGKXXXPP------NXGXPXXKTF 608
PP G G P PPP P G PP G PP + G
Sbjct: 325 PPIGNGSSNSSLPPPPPP----PRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLS 380
Query: 609 XPRGPKXPXXXKGPXWGXFXXXPPPPWXXSXKTXPPXXX--GXPPXXXXKXPXXXPPXXP 782
R P G PP S + PP PP P PP P
Sbjct: 381 SSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLP 440
Query: 783 PXXPPFFPXPXXFP 824
P P P P
Sbjct: 441 MGAPAAPPLPPSAP 454
Score = 30.3 bits (65), Expect = 0.41
Identities = 31/119 (26%), Positives = 32/119 (26%), Gaps = 2/119 (1%)
Frame = +3
Query: 444 PPPRGXGXXFXGPXPXPPPIXPXPXFFGGGXXFXPPXGG--KXXXPPNXGXPXXKTFXPR 617
PPP P P PPP P G PP G + PP P P
Sbjct: 376 PPPLSSSRAVSNP-PAPPPAIP-----GRSAPALPPLGNASRTSTPPVPTPPSLPPSAP- 428
Query: 618 GPKXPXXXKGPXWGXFXXXPPPPWXXSXKTXPPXXXGXPPXXXXKXPXXXPPXXPPXXP 794
P P P PP S PP G P PP P P
Sbjct: 429 -PSLPP--SAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 29.9 bits (64), Expect = 0.54
Identities = 34/132 (25%), Positives = 36/132 (27%), Gaps = 8/132 (6%)
Frame = +1
Query: 442 PPPPGXXGFXFXAPXPXPP------P*XPXPXFSGGGXXXXPPXGGXX--VXPQTXGXPX 597
PPPP P P P P P G PP G P P
Sbjct: 363 PPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPS 422
Query: 598 KKLXXQGAXXGPXPXKXPFGGXXPXSPPPPGXXPXKPPPPXXGGXPPXXL*XSPGFXPLX 777
+ P P G P +PP P P PP P P PL
Sbjct: 423 LPPSAPPSLPPSAPPSLPMGA--PAAPPLPPSAPIAPPLPA----------GMPAAPPLP 470
Query: 778 PPXXXPPFFPXP 813
P PP P P
Sbjct: 471 PAAPAPPPAPAP 482
Score = 27.9 bits (59), Expect = 2.2
Identities = 33/148 (22%), Positives = 40/148 (27%), Gaps = 10/148 (6%)
Frame = +2
Query: 437 KXPPPPGXXXXXXGPXPLXP-------PHXPXAPVFRGGGXXXXPPRGEXXFXPKPGXXP 595
+ PPPP G P+ P P P G PP+G P P
Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSA----PPPPP 365
Query: 596 XKNFXSKGPXXAXXXKRXXLGGFXXXXXXXLXTXXKNXPPXXXGX---TPPKXXKXPXXX 766
++ S G + PP TPP P
Sbjct: 366 PRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPP--VPTPPSL 423
Query: 767 PPXXPPXXPPLFSXXLXVSXKTLSPLSP 850
PP PP PP L + PL P
Sbjct: 424 PPSAPPSLPPSAPPSLPMGAPAAPPLPP 451
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/34 (32%), Positives = 12/34 (35%)
Frame = +1
Query: 637 PXKXPFGGXXPXSPPPPGXXPXKPPPPXXGGXPP 738
P P P +PP P P PP P P
Sbjct: 454 PIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.1 bits (67), Expect = 0.23
Identities = 21/71 (29%), Positives = 22/71 (30%), Gaps = 1/71 (1%)
Frame = +1
Query: 529 GGXXXXPPXGGXXVXPQTXGXPXKKLXXQGAXXGPXPXKXPFGGXXPXSPPP-PGXXPXK 705
GG P V PQ+ P P P P P S PP P P
Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMS--------APTPPPPPMSVPPPPSAPPMPAGPPSA 1730
Query: 706 PPPPXXGGXPP 738
PPPP P
Sbjct: 1731 PPPPLPASSAP 1741
Score = 31.1 bits (67), Expect = 0.23
Identities = 15/43 (34%), Positives = 15/43 (34%)
Frame = +1
Query: 667 PXSPPPPGXXPXKPPPPXXGGXPPXXL*XSPGFXPLXPPXXXP 795
P S PP PPPP PP P P PP P
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.1 bits (67), Expect = 0.23
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +1
Query: 658 GXXPXSPPPPGXXPXKPPPP 717
G P PPPPG P PPP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPP 26
Score = 26.6 bits (56), Expect = 5.0
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +1
Query: 442 PPPPGXXGFXFXAPXPXPPP 501
PPPP GF + P PPP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +1
Query: 682 PPGXXPXKPPPPXXGGXPP 738
PPG P PPPP G PP
Sbjct: 5 PPGNPP--PPPPPPGFEPP 21
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.5 bits (63), Expect = 0.71
Identities = 15/40 (37%), Positives = 16/40 (40%)
Frame = -1
Query: 563 FPPXGGXKXXPPPEKXGXGXYGGGXGXGAXKXXPXXPGGG 444
F GG PPP G G +GG G G GGG
Sbjct: 190 FGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGG 229
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 5.0
Identities = 20/89 (22%), Positives = 25/89 (28%)
Frame = +1
Query: 547 PPXGGXXVXPQTXGXPXKKLXXQGAXXGPXPXKXPFGGXXPXSPPPPGXXPXKPPPPXXG 726
PP PQ+ P + + P P P +PP P P P
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAP--PIPSKAPPIPSSLPPPAQPAAPV 181
Query: 727 GXPPXXL*XSPGFXPLXPPXXXPPFFPXP 813
PP P+ P PP P
Sbjct: 182 KSPPSAPSLPSAVPPMPPKVPPPPLSQAP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,062,078
Number of Sequences: 5004
Number of extensions: 34359
Number of successful extensions: 197
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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