Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_G14
(900 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.29
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.68
SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 29 0.90
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 29 1.2
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 2.1
SPAC3A11.04 |||siepin homolog|Schizosaccharomyces pombe|chr 1|||... 28 2.1
SPBC16E9.17c |rem1||meiosis-specific cyclin Rem1|Schizosaccharom... 27 4.8
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 8.4
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 8.4
SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 26 8.4
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 8.4
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.7 bits (66), Expect = 0.29
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 494 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 670
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVP 360
Query: 671 SNTTGT 688
+T +
Sbjct: 361 PTSTSS 366
Score = 30.7 bits (66), Expect = 0.29
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 494 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 670
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVP 414
Query: 671 SNTTGT 688
++ T
Sbjct: 415 PTSSST 420
Score = 29.9 bits (64), Expect = 0.52
Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +2
Query: 500 PKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSN 676
P T + S P P T+ TS S PP NST + +T V + SST P P++
Sbjct: 264 PPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNST-TPVTPTVPPTSTSSTSTPP-PPAS 321
Query: 677 TTGT 688
T+ T
Sbjct: 322 TSST 325
Score = 29.5 bits (63), Expect = 0.68
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +2
Query: 494 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 670
+TP P S S+P P T+ + S P T ST + + + S+T + P P
Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306
Query: 671 SNTTGT 688
+T +
Sbjct: 307 PTSTSS 312
Score = 29.1 bits (62), Expect = 0.90
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +2
Query: 494 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 670
+TP P S S+P P T+ + S P T ST S T + SS++ P+TP
Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249
Query: 671 S 673
+
Sbjct: 250 T 250
Score = 28.3 bits (60), Expect = 1.6
Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = +2
Query: 485 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP 661
L++ TP T P S S W TT T S T+ST +T T ++ +
Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657
Query: 662 LTPSNTTGT 688
T S++T T
Sbjct: 658 STSSSSTAT 666
>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 29.5 bits (63), Expect = 0.68
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -1
Query: 576 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 475
DLE N +V Q+ G G+FL G FG + FV+
Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444
>SPCC576.13 |swc5||chromatin remodeling complex subunit
Swc5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 215
Score = 29.1 bits (62), Expect = 0.90
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +1
Query: 220 ETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKE 363
ET K S K +K + + +++++ K NT++ A Q W+K KE
Sbjct: 128 ETPKKKHSLIRKRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKE 175
>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 708 FVHGGLKVPVVFEGVSGAITVDDTVIT 628
F H + V G+ GAIT+DD++IT
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIIT 133
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 27.9 bits (59), Expect = 2.1
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 187 QLYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 303
Q+++++V+ Y A+ EYL+EK I + K ++E
Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595
>SPAC3A11.04 |||siepin homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 236
Score = 27.9 bits (59), Expect = 2.1
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1
Query: 804 APRLHGLRWRPYLHQVS 754
A +LHG+RW Y H+VS
Sbjct: 185 ASKLHGMRWFMYTHKVS 201
>SPBC16E9.17c |rem1||meiosis-specific cyclin
Rem1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 402
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/35 (31%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 762 DEDMAANEDREALGHSGR-SFRFIPNFLHGTSXPY 863
DE ++ +E L H + RF+P++ H ++ PY
Sbjct: 135 DESVSVEYAKEILSHMEKLEIRFMPDYRHMSAQPY 169
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.8 bits (54), Expect = 8.4
Identities = 24/100 (24%), Positives = 40/100 (40%)
Frame = +1
Query: 580 STEDKQYLKLDNTKGSSDDRIIYGDSTADTFKHHWYLEPSMYESDVMFFVYNRESTVL*H 759
S + K + L N KG S T H YL + S ++ ST L
Sbjct: 887 SKKPKDAVNLGNNKGLSTKMFFSKFPTL-----HDYLLRELEVSVASLSSNDQPSTGLYP 941
Query: 760 LMKIWPPTKTVKPWGTAGEVSGLSPTFCMVHRPPTKRCCK 879
++ ++ + +P+G E +GLS ++ + R CK
Sbjct: 942 ILNMFSRLQYAQPYGNENEWTGLSQFEPLIFKCTASRICK 981
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 424 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 516
L+ K+D A K+ DQ H K+ +DK S
Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526
>SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 25.8 bits (54), Expect = 8.4
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = -2
Query: 323 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVAL 144
M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++
Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57
Query: 143 EASAHTARTKA 111
+A A T A
Sbjct: 58 NGAAKEAATAA 68
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 25.8 bits (54), Expect = 8.4
Identities = 22/82 (26%), Positives = 40/82 (48%)
Frame = -2
Query: 290 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 111
F S ++ F R+S + S TT+ + S SVL ++ A+E S TA ++
Sbjct: 3567 FVPSSVSRSFSYSRFSSGSLDSSSVFNSTTVSTASGISQGSVLSSTRAIE-SESTASHRS 3625
Query: 110 NKVSLILAQWLSLKASQQTTSK 45
+ +S + + LS A ++S+
Sbjct: 3626 SVLSELSSYDLSTSAFSSSSSE 3647
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,621,342
Number of Sequences: 5004
Number of extensions: 77564
Number of successful extensions: 311
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 273
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 309
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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