Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_F13
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomy... 163 4e-41
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 65 1e-11
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 64 2e-11
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 48 1e-06
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 40 3e-04
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 40 5e-04
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 39 0.001
SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomy... 26 7.9
>SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 194
Score = 163 bits (395), Expect = 4e-41
Identities = 79/171 (46%), Positives = 109/171 (63%), Gaps = 1/171 (0%)
Frame = +3
Query: 144 LQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYI 323
++ LL +Q DFV+ A+ S I ++K D++K ++ +M G++GDT FAEYI
Sbjct: 1 MESLLAVQGQDFVLTASSSSAVRGITVLKPDDDKSQILNSHNLMLYCGEAGDTTNFAEYI 60
Query: 324 AKNIQLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENG-PELYFMD 500
A NI LY +R+ L P A ASFTR+ LA LRS PY VN+L+ GY+ G PEL+++D
Sbjct: 61 AANISLYTLRHNLNLSPEATASFTRKQLATSLRSRKPYQVNILLAGYETNLGKPELFWLD 120
Query: 501 YLASSVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQEVHKR 653
YLA+ V+VP+A GY + LSI DRY+K D T EA I+K C E+ KR
Sbjct: 121 YLATCVRVPYACQGYSSFYCLSIFDRYYKPDLTIDEAVRIMKLCFDELKKR 171
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 64.9 bits (151), Expect = 1e-11
Identities = 39/162 (24%), Positives = 81/162 (50%)
Frame = +3
Query: 153 LLGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKN 332
++G+ D +++ AD + +I + +K++ IS + G + DT I+ N
Sbjct: 38 IVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMISSN 97
Query: 333 IQLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLAS 512
I+L+ + + A + +++L Y Y V +GGYD + GP L+ + S
Sbjct: 98 IELHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYLV---LGGYDCK-GPHLFTIAAHGS 153
Query: 513 SVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQ 638
S K+P+ A G G ++S+++ ++ D EA +++K+ ++
Sbjct: 154 SDKLPYVALGSGSLAAISVLETKYQPDLERHEAMELVKEAIE 195
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 64.1 bits (149), Expect = 2e-11
Identities = 44/171 (25%), Positives = 78/171 (45%), Gaps = 2/171 (1%)
Frame = +3
Query: 153 LLGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAK- 329
+L ++ D VMIAAD S+ + D E++ K+ D ++G GD D Q + K
Sbjct: 46 VLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQIQRLLEKL 105
Query: 330 NIQLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKEN-GPELYFMDYL 506
I+ +GY L PS + + L PY+ +++ G D EN P + F D
Sbjct: 106 EIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENKEPYVAFADLR 165
Query: 507 ASSVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQEVHKRXF 659
++ P A G+ +L+L ++ + D +T + + + + E + F
Sbjct: 166 GTTYSAPAIATGFAMHLALPMLRKATDDDRWKTLSKESARATIDECMRVLF 216
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 48.4 bits (110), Expect = 1e-06
Identities = 30/119 (25%), Positives = 54/119 (45%)
Frame = +3
Query: 156 LGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 335
+ I + F ++A D + + I + +++++ D LV+G G D + I + I
Sbjct: 17 VAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVKRIQQRI 76
Query: 336 QLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLAS 512
LY + ++ + A R L Y + PY+V + G DKE E+Y D + S
Sbjct: 77 DLYHDNHERKMSAQSCACMVRTLL--YGKRFFPYYVYTTVAGIDKEGKGEIYSFDPVGS 133
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 40.3 bits (90), Expect = 3e-04
Identities = 27/115 (23%), Positives = 51/115 (44%), Gaps = 3/115 (2%)
Frame = +3
Query: 156 LGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 335
LGI D +++AA++ + ++ ++ EK+Y I D ++ V G + D N Y +
Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVG 94
Query: 336 QLYKMRNGYELGPSAAASFT---RRNLAEYLRSSTPYFVNVLMGGYDKENGPELY 491
Q Y E+ ++ +Y P+ V+ L G+D G +L+
Sbjct: 95 QQYLQTFNEEMPCEQLVRRVCDLKQGYTQY-GGLRPFGVSFLYAGWDHIRGYQLF 148
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 39.9 bits (89), Expect = 5e-04
Identities = 31/152 (20%), Positives = 69/152 (45%)
Frame = +3
Query: 180 VMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNG 359
+++ D S +I +K+ +I+ L+ + G + D + + +L+++RN
Sbjct: 73 IVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRN- 131
Query: 360 YELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLASSVKVPFAAH 539
EL +AAS N+ Y + ++ G K G LY++D + +K +
Sbjct: 132 KELISVSAASKILSNIT-YSYKGYGLSMGTMLAGTGK-GGTALYYIDSDGTRLKGDLFSV 189
Query: 540 GYGGYLSLSIMDRYHKKDATETEAYDILKKCV 635
G G + ++D ++ D ++ EA + ++ +
Sbjct: 190 GSGSTFAYGVLDSGYRWDLSKQEALYLAQRSI 221
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 38.7 bits (86), Expect = 0.001
Identities = 39/171 (22%), Positives = 72/171 (42%), Gaps = 8/171 (4%)
Frame = +3
Query: 156 LGIQCNDFVMIAADQSNSHSIMIMKDDEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 335
+G++ D V++ ++ + +M EK+++I + + G + D E+
Sbjct: 38 IGVKTKDAVVLGVEKRLTSPLM-ESHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQT 96
Query: 336 Q----LYKMRNGYELGPSA----AASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELY 491
Q Y G E + A F E S P+ V +L+ G D E+GP+LY
Sbjct: 97 QNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIAGID-EHGPQLY 155
Query: 492 FMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKKDATETEAYDILKKCVQEV 644
+ + + A G G + S + + KD T EA ++ K +++V
Sbjct: 156 HSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLILKVLRQV 206
>SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 764
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +1
Query: 277 VSLVTQETQINLLNILQKIFSCIKCVMGMNWGPQLQPVS 393
VS+ ++ + LLN+L + SCI + P++ PVS
Sbjct: 673 VSIENSKSDMKLLNVLSRYTSCIGPGLFDIHSPRVPPVS 711
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,044,888
Number of Sequences: 5004
Number of extensions: 60230
Number of successful extensions: 155
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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