Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_E17
(928 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 205 6e-54
SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 205 8e-54
SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 29 0.93
SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 29 1.2
SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|ch... 27 2.8
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 27 5.0
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 6.6
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 8.7
>SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 205 bits (501), Expect = 6e-54
Identities = 95/178 (53%), Positives = 133/178 (74%)
Frame = +3
Query: 132 IVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDA 311
+VDPF+RK+WYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLADLQ D
Sbjct: 20 VVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQKDE-- 77
Query: 312 ERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRV 491
E +FRK +L E +QG++ L +F+G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RV
Sbjct: 78 EHAFRKVKLRVEDIQGKSCLTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRV 137
Query: 492 FCIGFTNKDSLSQRKTCYAQHTQVRAIRKKMCEIITRDVTNSELREVVNKLIPDSIAK 665
F IGFT + + +KT YAQ +Q+RAIR+KM ++I ++ +RE+V KLIP+ I +
Sbjct: 138 FVIGFTRRRANQVKKTTYAQSSQIRAIRQKMFQVIQNQTSSCSMRELVQKLIPEVIGR 195
Score = 34.3 bits (75), Expect = 0.025
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +1
Query: 664 RDIEKACHGIYPLRDVCIRKVXVLNRPRXXISXXME 771
R+IE+A I+PL++V +RKV +L P+ +E
Sbjct: 195 REIERATGSIFPLQNVLVRKVKILKAPKHDAQKLLE 230
>SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 205 bits (500), Expect = 8e-54
Identities = 95/178 (53%), Positives = 130/178 (73%)
Frame = +3
Query: 132 IVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDA 311
+VDPF+RKDWYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLADLQ D
Sbjct: 20 VVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQKDE-- 77
Query: 312 ERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRV 491
E SFRK +L E +QG++ L +F+G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+
Sbjct: 78 EHSFRKVKLRVEDIQGKSCLTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRI 137
Query: 492 FCIGFTNKDSLSQRKTCYAQHTQVRAIRKKMCEIITRDVTNSELREVVNKLIPDSIAK 665
F IGFT++ +KT YAQ +Q+RAI +KM ++I ++E+V KLIP+ I +
Sbjct: 138 FVIGFTSRRVNQVKKTTYAQSSQIRAIHQKMFQVIQNQANGCSMKELVQKLIPEVIGR 195
Score = 36.3 bits (80), Expect = 0.006
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +1
Query: 664 RDIEKACHGIYPLRDVCIRKVXVLNRPRXXISXXME 771
R IEKA + IYPL++V +RKV +L P+ +E
Sbjct: 195 RAIEKATNNIYPLQNVFVRKVKILKAPKHDAQKLLE 230
>SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1019
Score = 29.1 bits (62), Expect = 0.93
Identities = 27/111 (24%), Positives = 47/111 (42%), Gaps = 2/111 (1%)
Frame = +3
Query: 210 TLVNRTQG-TKIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHG 386
T VN+ TK+A KG +V++ L+ DAE + + F I EY++ N
Sbjct: 82 TSVNKANMLTKLAET--KGAAADVNI--LKRTKDAEENKKLFEKIIEYIRATNKKVGVFP 137
Query: 387 MDLTTDKLRWMVKKWQTLIE-ANIDVKTTDGYVLRVFCIGFTNKDSLSQRK 536
D T K + +W ++ E + D + C+ ++ L+ K
Sbjct: 138 KDKTQGK---FINEWDSIFEPVKSEFNLVDASLGLAKCLAIKDEQELANIK 185
>SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 308
Score = 28.7 bits (61), Expect = 1.2
Identities = 22/70 (31%), Positives = 36/70 (51%), Gaps = 2/70 (2%)
Frame = +3
Query: 267 VFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMV--KKWQTL 440
VF+V + + D R+ + +A Q R V C H MDL + LR +V ++ L
Sbjct: 86 VFDVESREFERDLVTFRNCLE-ATVANSPQAR-VFCLIHKMDLVQEDLRDLVFEERKAIL 143
Query: 441 IEANIDVKTT 470
+E + D++TT
Sbjct: 144 LETSKDLETT 153
>SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 743
Score = 27.5 bits (58), Expect = 2.8
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = -1
Query: 715 CKHRAEGRCHGRPSRCPLAMESGINLFTTSLSSELVTSRVMISHIF 578
C HR G + C A S NLF S+ + + ++++ H F
Sbjct: 485 CVHRGTGSITDSQALCIAAARSITNLFVESVDLQPLIMKIIMYHAF 530
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 26.6 bits (56), Expect = 5.0
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -3
Query: 434 LPFFNHPPELIGCEVHAVEVAEHITS 357
L H PEL+ C +VA+H+TS
Sbjct: 181 LQSMGHSPELVQCITCLPDVADHLTS 206
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/36 (27%), Positives = 22/36 (61%)
Frame = +3
Query: 576 KKMCEIITRDVTNSELREVVNKLIPDSIAKGHREGL 683
+K ++ +++ N++L+ +NK I I + H+E L
Sbjct: 415 QKALYVLKKELLNAQLQHKINKEIEQKITQRHKEYL 450
>SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1309
Score = 25.8 bits (54), Expect = 8.7
Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +2
Query: 494 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNQKENV 586
LHW+ L+ +Q + P H+GQ S + NV
Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNV 1194
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,339,585
Number of Sequences: 5004
Number of extensions: 67576
Number of successful extensions: 188
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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