Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_D08
(885 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 384 e-109
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 374 e-106
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 266 2e-73
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 233 2e-63
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.9
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 384 bits (945), Expect = e-109
Identities = 183/190 (96%), Positives = 184/190 (96%)
Frame = +1
Query: 169 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 348
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 349 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 528
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 529 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 708
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 709 IGYXPSCCRF 738
IGY P+ F
Sbjct: 181 IGYNPAAVAF 190
Score = 52.4 bits (120), Expect = 5e-09
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +3
Query: 744 PISGWHGDNMLEPSTKMPWFKG 809
PISGWHGDNMLE S+KMPWFKG
Sbjct: 192 PISGWHGDNMLEVSSKMPWFKG 213
Score = 21.8 bits (44), Expect = 8.6
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2
Query: 704 RRLATTPAAVAFVP 745
+++ PAAVAFVP
Sbjct: 179 KKIGYNPAAVAFVP 192
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 374 bits (919), Expect = e-106
Identities = 178/190 (93%), Positives = 181/190 (95%)
Frame = +1
Query: 169 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 348
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 349 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 528
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 529 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 708
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 709 IGYXPSCCRF 738
IGY + F
Sbjct: 181 IGYNTASVAF 190
Score = 52.8 bits (121), Expect = 4e-09
Identities = 20/31 (64%), Positives = 23/31 (74%)
Frame = +3
Query: 744 PISGWHGDNMLEPSTKMPWFKGCRXTXRKXN 836
PISGWHGDNMLEPS K PW+KG + + N
Sbjct: 192 PISGWHGDNMLEPSPKTPWYKGWKVERKDGN 222
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 266 bits (651), Expect = 2e-73
Identities = 127/133 (95%), Positives = 128/133 (96%)
Frame = +1
Query: 340 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 519
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 520 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 699
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 700 IKKIGYXPSCCRF 738
IKKIGY P+ F
Sbjct: 121 IKKIGYNPAAVAF 133
Score = 52.4 bits (120), Expect = 5e-09
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +3
Query: 744 PISGWHGDNMLEPSTKMPWFKG 809
PISGWHGDNMLE S+KMPWFKG
Sbjct: 135 PISGWHGDNMLEVSSKMPWFKG 156
Score = 21.8 bits (44), Expect = 8.6
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2
Query: 704 RRLATTPAAVAFVP 745
+++ PAAVAFVP
Sbjct: 122 KKIGYNPAAVAFVP 135
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 233 bits (569), Expect = 2e-63
Identities = 111/117 (94%), Positives = 112/117 (95%)
Frame = +1
Query: 388 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 567
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 568 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYXPSCCRF 738
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGY P+ F
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF 117
Score = 52.4 bits (120), Expect = 5e-09
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +3
Query: 744 PISGWHGDNMLEPSTKMPWFKG 809
PISGWHGDNMLE S+KMPWFKG
Sbjct: 119 PISGWHGDNMLEVSSKMPWFKG 140
Score = 21.8 bits (44), Expect = 8.6
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2
Query: 704 RRLATTPAAVAFVP 745
+++ PAAVAFVP
Sbjct: 106 KKIGYNPAAVAFVP 119
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 2e-04
Identities = 32/106 (30%), Positives = 47/106 (44%)
Frame = +1
Query: 427 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 606
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 607 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYXPSCCRFRA 744
+IV +NK+D + ++E K + I+++G C + A
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELGGEIQCVKISA 290
Score = 25.4 bits (53), Expect = 0.70
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 181 KTHINIVVIGHVDSGKST 234
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 190 INIVVIGHVDSGKST 234
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 412 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 513
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 412 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 513
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 248,767
Number of Sequences: 438
Number of extensions: 4992
Number of successful extensions: 23
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 28766349
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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