Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_D03
(931 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 85 1e-17
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 61 2e-10
SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 52 9e-08
SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 48 2e-06
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 44 4e-05
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 40 5e-04
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 39 0.001
SPAC110.03 |cdc42||Rho family GTPase Cdc42|Schizosaccharomyces p... 33 0.076
SPAC16.01 |rho2||Rho family GTPase Rho2|Schizosaccharomyces pomb... 33 0.076
SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 32 0.10
SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces po... 29 0.93
SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 29 0.93
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 29 1.2
SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 28 2.2
SPAP8A3.06 |||U2AF small subunit, U2AF-23|Schizosaccharomyces po... 27 2.8
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 27 5.0
SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces p... 26 8.7
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 85.4 bits (202), Expect = 1e-17
Identities = 38/54 (70%), Positives = 45/54 (83%)
Frame = +2
Query: 485 GXDGIIIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKKVVDF 646
G GIIIVYD T QDSF+NVKQWL+EIDRYA + VN+LLVGNK D+ KKVV++
Sbjct: 80 GAHGIIIVYDVTDQDSFNNVKQWLQEIDRYAVEGVNRLLVGNKSDMVDKKVVEY 133
Score = 58.4 bits (135), Expect = 1e-09
Identities = 27/47 (57%), Positives = 36/47 (76%)
Frame = +3
Query: 630 KRLSTLXTAKQYAEQLGIPXLETSAKNSTNVEQAFMTMAAXIKARVG 770
K++ AK++A+ L IP LETSAK+STNVEQAF+TM+ IK R+G
Sbjct: 128 KKVVEYSVAKEFADSLNIPFLETSAKDSTNVEQAFLTMSRQIKERMG 174
Score = 54.8 bits (126), Expect = 2e-08
Identities = 28/59 (47%), Positives = 32/59 (54%)
Frame = +1
Query: 301 VGTXCLSXXCAXDTXTDSXXXXIGXXXXXXXVNVXGNTIE*QIWDTXGQERXTTITSSY 477
VG CL A DT T+S IG + G T++ QIWDT GQER TITSSY
Sbjct: 19 VGKSCLLLRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSY 77
>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 200
Score = 61.3 bits (142), Expect = 2e-10
Identities = 25/65 (38%), Positives = 40/65 (61%)
Frame = +2
Query: 485 GXDGIIIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKKVVDFXYS*AI 664
G GI+++YD T + SF NV+ W ++++A +NV K+L+GNKCD ++ V F A+
Sbjct: 81 GAMGILLLYDVTDKKSFDNVRTWFSNVEQHASENVYKILIGNKCDCEDQRQVSFEQGQAL 140
Query: 665 CGTAG 679
G
Sbjct: 141 ADELG 145
Score = 48.0 bits (109), Expect = 2e-06
Identities = 24/59 (40%), Positives = 31/59 (52%)
Frame = +1
Query: 301 VGTXCLSXXCAXDTXTDSXXXXIGXXXXXXXVNVXGNTIE*QIWDTXGQERXTTITSSY 477
VG CL + D+ T S IG + + G I+ QIWDT GQER TIT++Y
Sbjct: 20 VGKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAY 78
Score = 37.9 bits (84), Expect = 0.002
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = +3
Query: 666 AEQLGIPXLETSAKNSTNVEQAFMTMAAXIK 758
A++LG+ LE SAK + NV++AF T+A IK
Sbjct: 141 ADELGVKFLEASAKTNVNVDEAFFTLAREIK 171
>SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 214
Score = 52.4 bits (120), Expect = 9e-08
Identities = 25/52 (48%), Positives = 33/52 (63%)
Frame = +2
Query: 485 GXDGIIIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKKVV 640
G G +IVYD T Q SF NV +WL+E+ +A N+ +LVGNK DL + V
Sbjct: 82 GAVGALIVYDITKQSSFDNVGRWLKELREHADSNIVIMLVGNKTDLLHLRAV 133
Score = 33.5 bits (73), Expect = 0.043
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +1
Query: 367 IGXXXXXXXVNVXGNTIE*QIWDTXGQERXTTITSSY 477
IG + + I+ QIWDT GQER ITS+Y
Sbjct: 43 IGVEFATRNIVLDNKKIKAQIWDTAGQERYRAITSAY 79
Score = 31.5 bits (68), Expect = 0.18
Identities = 16/43 (37%), Positives = 28/43 (65%)
Frame = +3
Query: 627 LKRLSTLXTAKQYAEQLGIPXLETSAKNSTNVEQAFMTMAAXI 755
L+ +ST A+ +A + + +ETSA +++NVE+AF T+ I
Sbjct: 130 LRAVST-EEAQAFAAENNLSFIETSAMDASNVEEAFQTVLTEI 171
>SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 201
Score = 47.6 bits (108), Expect = 2e-06
Identities = 22/47 (46%), Positives = 32/47 (68%)
Frame = +2
Query: 500 IIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKKVV 640
IIVYD T+ +SF N ++W+E++ D+V +LVGNK DL K+ V
Sbjct: 88 IIVYDITNHNSFVNTEKWIEDVRAERGDDVIIVLVGNKTDLADKRQV 134
Score = 31.5 bits (68), Expect = 0.18
Identities = 17/59 (28%), Positives = 25/59 (42%)
Frame = +1
Query: 301 VGTXCLSXXCAXDTXTDSXXXXIGXXXXXXXVNVXGNTIE*QIWDTXGQERXTTITSSY 477
VG L D ++ IG + + T+ Q+WDT GQER ++ SY
Sbjct: 22 VGKTSLITRFMYDQFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSY 80
Score = 26.2 bits (55), Expect = 6.6
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = +3
Query: 630 KRLSTLXTAKQYAEQLGIPXLETSAKNSTNVEQAFMTMA 746
KR T ++ A++L I +ETSAK NV+ F +A
Sbjct: 131 KRQVTQEEGEKKAKELKIMHMETSAKAGHNVKLLFRKIA 169
>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 234
Score = 43.6 bits (98), Expect = 4e-05
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +2
Query: 485 GXDGIIIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKKVV 640
G G ++VYD T++DSF + WL +I A + +L G+K DL ++ V
Sbjct: 85 GAAGAVLVYDVTNKDSFEELSSWLSDIRAMAPSTICVVLAGSKSDLQNQRQV 136
Score = 29.1 bits (62), Expect = 0.93
Identities = 18/62 (29%), Positives = 27/62 (43%), Gaps = 4/62 (6%)
Frame = +1
Query: 304 GTXCLSXXCAXDTXTDSXXXXIGXXXXXXXVNVX-GNT---IE*QIWDTXGQERXTTITS 471
G CL + D +G ++V GN I+ QIWDT GQE+ ++
Sbjct: 21 GKSCLLQRFVKNQWDDQVSHTVGIDFASRIISVGMGNQQKRIKLQIWDTAGQEKFRSVAR 80
Query: 472 SY 477
+Y
Sbjct: 81 NY 82
>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 211
Score = 39.9 bits (89), Expect = 5e-04
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +2
Query: 500 IIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKK 634
I+VYD T S K W++E+ R A + + L GNK DL ++
Sbjct: 92 IVVYDITQAASLEKAKSWIKELQRQAPEGIVIALAGNKLDLAQER 136
Score = 29.5 bits (63), Expect = 0.71
Identities = 18/60 (30%), Positives = 25/60 (41%), Gaps = 1/60 (1%)
Frame = +1
Query: 301 VGTXCLSXXCAXDTXTDSXXXXIGXXXXXXXVNVXGNT-IE*QIWDTXGQERXTTITSSY 477
VG L D D IG + + NT ++ +IWDT GQER ++ Y
Sbjct: 25 VGKSSLVLRFVKDQFDDYRESTIGAAFLTQTLPIDENTSVKLEIWDTAGQERYKSLAPMY 84
>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 216
Score = 38.7 bits (86), Expect = 0.001
Identities = 17/46 (36%), Positives = 29/46 (63%)
Frame = +2
Query: 500 IIVYDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGNKCDLTTKKV 637
II++D T + ++ NV W ++ R C+N+ +L GNK D+ +KV
Sbjct: 86 IIMFDVTSRITYKNVPHWWRDLVR-VCENIPIVLCGNKVDVKERKV 130
>SPAC110.03 |cdc42||Rho family GTPase Cdc42|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 192
Score = 32.7 bits (71), Expect = 0.076
Identities = 22/63 (34%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +2
Query: 437 QXDRSGLRPSPXHTTXGXDGIIIVYDSTHQDSFSNVKQ-WLEEIDRYACDNVNKLLVGNK 613
Q D LRP T D ++ + T SF NVK+ W E+ + C V L+VG +
Sbjct: 61 QEDYDRLRPLSYPQT---DVFLVCFSVTSPASFENVKEKWFPEVHHH-CPGVPCLIVGTQ 116
Query: 614 CDL 622
DL
Sbjct: 117 IDL 119
>SPAC16.01 |rho2||Rho family GTPase Rho2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 200
Score = 32.7 bits (71), Expect = 0.076
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +2
Query: 497 IIIVYDSTHQDSFSNVK-QWLEEIDRYACDNVNKLLVGNKCDLTTKKV 637
I++ + DS NV +W+EEI+ C NV +LVG K DL + V
Sbjct: 83 ILVGFAIDSPDSLENVSTKWIEEINTL-CPNVPFILVGMKADLRSDPV 129
>SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 205
Score = 32.3 bits (70), Expect = 0.10
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = +3
Query: 630 KRLSTLXTAKQYAEQLG-IPXLETSAKNSTNVEQAFMTMA 746
KR+ + A + + G IP ETSAK + NV++AF T+A
Sbjct: 133 KRMVSKSKALAFCQARGEIPYFETSAKEAINVQEAFETVA 172
Score = 25.8 bits (54), Expect = 8.7
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +1
Query: 367 IGXXXXXXXVNVXGNTIE*QIWDTXGQERXTTI 465
IG V V + Q+WDT GQER ++
Sbjct: 41 IGADFLTKEVLVDDKVVTLQLWDTAGQERFQSL 73
>SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 205
Score = 29.1 bits (62), Expect = 0.93
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 400 VXGNTIE*QIWDTXGQERXTTITS 471
V GN+IE +WDT GQE + S
Sbjct: 56 VDGNSIELSLWDTAGQEEYDQLRS 79
>SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr
1|||Manual
Length = 208
Score = 29.1 bits (62), Expect = 0.93
Identities = 16/59 (27%), Positives = 23/59 (38%)
Frame = +1
Query: 301 VGTXCLSXXCAXDTXTDSXXXXIGXXXXXXXVNVXGNTIE*QIWDTXGQERXTTITSSY 477
VG CL + IG V V + Q+WDT GQER ++ ++
Sbjct: 19 VGKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQLWDTAGQERFQSLGMAF 77
Score = 26.6 bits (56), Expect = 5.0
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 485 GXDGIIIVYDSTHQDSFSNVKQWLEE 562
G D +IVY+ + SF +V+ W +E
Sbjct: 80 GADCCVIVYNVNNSKSFDSVENWRQE 105
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 28.7 bits (61), Expect = 1.2
Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 2/58 (3%)
Frame = +2
Query: 455 LRPSPXHTTXGXDGIIIVYDSTHQDSFSNVKQWLEEI--DRYACDNVNKLLVGNKCDL 622
+RP H G G+I V DS + S +Q L I DR D + L++ NK DL
Sbjct: 78 IRPLWRHYFTGTKGLIFVVDSADSNRISEARQELHRIISDREMRDCL-LLVLANKQDL 134
>SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 27.9 bits (59), Expect = 2.2
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +3
Query: 630 KRLSTLXTAKQYAEQLGIPXLETSAKNSTNVEQAFMTMAAXIK 758
+R+ + +Q A+ + +ETSAK NVE+AF ++ I+
Sbjct: 128 ERVVSRAEGEQLAKSMHCLYVETSAKLRLNVEEAFYSLVRTIR 170
>SPAP8A3.06 |||U2AF small subunit, U2AF-23|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 216
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 509 YDSTHQDSFSNVKQWLEEIDRYACDNVNKLLVGN 610
+D+ ++D F ++ E CDNV LVGN
Sbjct: 73 FDAFYEDMFCEFSKYGEVEQLVVCDNVGDHLVGN 106
>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 504
Score = 26.6 bits (56), Expect = 5.0
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 665 CGTAGHSVPRDVXEELYER 721
CGT G++ P V +ELY +
Sbjct: 254 CGTVGYAAPEIVNDELYSK 272
>SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 200
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +1
Query: 394 VNVXGNTIE*QIWDTXGQE 450
V V G IE +WDT GQE
Sbjct: 47 VEVDGRHIELALWDTAGQE 65
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,206,170
Number of Sequences: 5004
Number of extensions: 28691
Number of successful extensions: 92
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 76
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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