Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_C17
(960 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 30 0.42
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.74
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.97
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 29 1.3
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 5.2
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 5.2
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 27 5.2
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 5.2
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 5.2
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.9
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 6.9
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 9.1
SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc... 26 9.1
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 9.1
SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|ch... 26 9.1
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 30.3 bits (65), Expect = 0.42
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 8/80 (10%)
Frame = +3
Query: 381 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 536
K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186
Query: 537 AKKVSSNVQETNEKLAPKIK 596
K + T +KL KI+
Sbjct: 187 YKDMEQENASTIQKLTSKIR 206
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 29.5 bits (63), Expect = 0.74
Identities = 14/65 (21%), Positives = 31/65 (47%)
Frame = +3
Query: 381 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 560
KA + LE+ ++ E + EE+ H + T+ + + + +QE ++ K +
Sbjct: 353 KACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEKSRVDKMIDEYR 412
Query: 561 QETNE 575
Q+ +E
Sbjct: 413 QKLSE 417
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 29.1 bits (62), Expect = 0.97
Identities = 17/69 (24%), Positives = 38/69 (55%)
Frame = +3
Query: 381 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 560
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 561 QETNEKLAP 587
+ ++ ++P
Sbjct: 186 RSSSRSISP 194
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 28.7 bits (61), Expect = 1.3
Identities = 22/91 (24%), Positives = 42/91 (46%), Gaps = 3/91 (3%)
Frame = +3
Query: 363 LGXANGKAKEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQK 533
+ A KA+++LEQ+ + E L K H + E+ + +EKL A ++ + S++
Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111
Query: 534 LAKKVSSNVQETNEKLAPKIKAAYDDXXXNN 626
L + + E A +++A NN
Sbjct: 112 L-DHYRAIISSKRELRAQEMEAKRKQDSYNN 141
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.6 bits (56), Expect = 5.2
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -2
Query: 434 LGRALDVLPRLFQSLLGLAVRVSERXPGDSW 342
+G + L RLF SL GL ++ PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 5.2
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +2
Query: 179 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 298
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 26.6 bits (56), Expect = 5.2
Identities = 16/71 (22%), Positives = 36/71 (50%)
Frame = +3
Query: 396 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 575
++ Q+IE T L K D+E++ +++ + V + Q+ ++++ +Q+T E
Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553
Query: 576 KLAPKIKAAYD 608
L+ K + D
Sbjct: 554 VLSKSSKESDD 564
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 5.2
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +2
Query: 173 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 262
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 26.6 bits (56), Expect = 5.2
Identities = 17/55 (30%), Positives = 25/55 (45%)
Frame = +3
Query: 366 GXANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQ 530
G KAKE ++ R +RTA E+RK +E+ R + A Q + Q
Sbjct: 159 GADEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRAERAAEAQRVAGKEQ 213
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 6.9
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +2
Query: 86 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 265
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 266 SKDA 277
SKDA
Sbjct: 230 SKDA 233
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 6.9
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +3
Query: 396 LEQSRQNIERTAEELRKAHPDVEK 467
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 9.1
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
Frame = +3
Query: 543 KVSSNVQET--NEKLAPKIKAAYD 608
+V N++ET EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322
>SPAC23H4.11c |cnl2||centromere localized protein
Cnl2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 25.8 bits (54), Expect = 9.1
Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +3
Query: 387 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 560
++ L + R NI ++ + K+ D + N L+ A+ + V+E ++
Sbjct: 53 QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112
Query: 561 QETNEKLAPKIKAAY 605
E NE+L+ +++AY
Sbjct: 113 SEINERLSEAVQSAY 127
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 9.1
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 143 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 319
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 320 LQQL 331
Q+
Sbjct: 73 NNQI 76
>SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 401
Score = 25.8 bits (54), Expect = 9.1
Identities = 19/73 (26%), Positives = 30/73 (41%)
Frame = +3
Query: 411 QNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPK 590
+N+ +KA DV + A + K +QN + +QK +VS E EK
Sbjct: 232 ENLGELTRNWQKAMDDVTEKF-ASKSKEYEDLQNELDATQKRLSRVSDLEHEVKEKTLLI 290
Query: 591 IKAAYDDXXXNNH 629
K ++ N H
Sbjct: 291 GKLQHEAVVLNEH 303
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,269,249
Number of Sequences: 5004
Number of extensions: 33950
Number of successful extensions: 157
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 491307756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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