Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_B19
(873 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 38 0.002
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 33 0.040
SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar... 30 0.49
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 29 1.1
SPBC26H8.02c |sec9||SNAP-25 homologue, t-SNARE component Sec9|Sc... 29 1.1
SPCC663.14c |||TRP-like ion channel|Schizosaccharomyces pombe|ch... 29 1.1
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 28 2.0
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 3.5
SPBP4H10.14c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 3.5
SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces... 27 3.5
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 27 4.6
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 4.6
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 27 4.6
SPAC4H3.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.1
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 26 6.1
SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|ch... 26 6.1
SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe... 26 8.1
SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|... 26 8.1
SPAC664.08c |||traub transciption factor family protein |Schizos... 26 8.1
SPAC17C9.12 |||MSP domain|Schizosaccharomyces pombe|chr 1|||Manual 26 8.1
>SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 658
Score = 37.9 bits (84), Expect = 0.002
Identities = 26/103 (25%), Positives = 42/103 (40%)
Frame = +2
Query: 347 GPRPNMMNKNFRPRNDFNEVKNDYNTKNDGNQNDFGGPKQFRPRNNFNNGNQPPKKNNFN 526
G + + + N+ N +YN N GN N +Q + ++ G N+ +
Sbjct: 551 GMASSFLGSSGNNNNNNNSNSGNYNNNNSGNNN-----QQHQQSSSSLQGLASSFLNSSS 605
Query: 527 GDKSPGNMQYGNKNDFGGPKQQNYNKNYGPKTYNNQNCYGNEQ 655
G+ + N N ++G QNYN N NN + GN Q
Sbjct: 606 GNSNKQNYNNNNNQNYGNNNNQNYNNN------NNSSQGGNSQ 642
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/63 (23%), Positives = 25/63 (39%)
Frame = +2
Query: 419 NTKNDGNQNDFGGPKQFRPRNNFNNGNQPPKKNNFNGDKSPGNMQYGNKNDFGGPKQQNY 598
++ N+ N ++ N NN + K ++ +G S GN N+ NY
Sbjct: 515 SSSNNSNSSNNNSNTSNNNSNTSNNESMVSKLSSLSGMASSFLGSSGNNNNNNNSNSGNY 574
Query: 599 NKN 607
N N
Sbjct: 575 NNN 577
Score = 26.2 bits (55), Expect = 6.1
Identities = 24/97 (24%), Positives = 31/97 (31%), Gaps = 1/97 (1%)
Frame = +2
Query: 479 NNFNNGNQPPKKNNFNGDKSPGNMQYGNKNDF-GGPKQQNYNKNYGPKTYNNQNCYGNEQ 655
NN N N +N S + G + F G N N N YNN N GN
Sbjct: 525 NNSNTSNNNSNTSNNESMVSKLSSLSGMASSFLGSSGNNNNNNNSNSGNYNNNNS-GNNN 583
Query: 656 PEFIPRQSYSPNSVQHSLNDRKLQSKKAKHPGDSLLN 766
+ S LN S K + ++ N
Sbjct: 584 QQHQQSSSSLQGLASSFLNSSSGNSNKQNYNNNNNQN 620
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 33.5 bits (73), Expect = 0.040
Identities = 31/108 (28%), Positives = 48/108 (44%), Gaps = 13/108 (12%)
Frame = +2
Query: 389 NDFNEVKNDYNTKNDG-NQNDFGGPKQFRPRNNFNNGNQPP--KKNNFNGDKSPGNMQYG 559
N ++ ++ +N N G N+N+ G P+ R N+NN P N + ++SP N
Sbjct: 315 NKYSHYQHGFNYNNSGNNRNESGHPRFRNSRRNYNNQGAYPTYMSNGRSANQSPRNNPQ- 373
Query: 560 NKNDFGGP------KQQNYNKNYGPKTY---NNQNCYGN-EQPEFIPR 673
N N+ P Q Y + YG Y N YG QP ++P+
Sbjct: 374 NVNNGSTPIQIPVSLQTPYGQVYGQPQYIVDPNMVQYGPILQPGYVPQ 421
>SPBP8B7.16c |dbp2||ATP-dependent RNA helicase
Dbp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 29.9 bits (64), Expect = 0.49
Identities = 12/49 (24%), Positives = 26/49 (53%)
Frame = +2
Query: 509 KKNNFNGDKSPGNMQYGNKNDFGGPKQQNYNKNYGPKTYNNQNCYGNEQ 655
+ N ++G+ + Y ++ +GG + NY++ G +N+ YG +Q
Sbjct: 4 RDNEYSGNYNGKEDGYNSRGRYGGGYRNNYSRGGGRGGFNDGASYGYDQ 52
>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 964
Score = 28.7 bits (61), Expect = 1.1
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 5/36 (13%)
Frame = +2
Query: 470 RPRNNFNNGNQPPKK-----NNFNGDKSPGNMQYGN 562
R ++GNQPP++ N F+ P NMQ GN
Sbjct: 569 RNTQTLSSGNQPPQQSGPNPNEFSMSMDPANMQQGN 604
>SPBC26H8.02c |sec9||SNAP-25 homologue, t-SNARE component
Sec9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 419
Score = 28.7 bits (61), Expect = 1.1
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +2
Query: 515 NNFNGDKSPGNMQYGNKNDFGGPKQQNYNKNYGPKTYNNQNCYGN 649
N++N D + N YG+ N++G N NYG +Y N YG+
Sbjct: 46 NSYN-DNNNSNSTYGSSNNYGNYGSSN---NYG--SYGASNTYGS 84
Score = 27.9 bits (59), Expect = 2.0
Identities = 18/57 (31%), Positives = 21/57 (36%), Gaps = 2/57 (3%)
Frame = +2
Query: 479 NNFNNGNQP-PKKNNFNGDKSPGNM-QYGNKNDFGGPKQQNYNKNYGPKTYNNQNCY 643
N+ NN N NN+ S N YG N +G N NYG N Y
Sbjct: 49 NDNNNSNSTYGSSNNYGNYGSSNNYGSYGASNTYGSNGSSNNYGNYGATNSNGDAGY 105
>SPCC663.14c |||TRP-like ion channel|Schizosaccharomyces pombe|chr
3|||Manual
Length = 687
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/57 (31%), Positives = 33/57 (57%), Gaps = 1/57 (1%)
Frame = -3
Query: 691 VWTVRLPRN-KFRLLIPIAILVVVCFRPIILVIILLFRTAKIIFIAILHISW*FVSI 524
V+ V L R F LLIPIA + + + II +I+++ + F+A+ + W F+++
Sbjct: 526 VFLVALTRVVSFALLIPIAFDLAIIPKVIIAIILIIIQGLVFSFMALWSL-WNFITM 581
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +2
Query: 611 GPKTYN-NQNCYGNEQPEFIPRQSYSPNSVQHSL 709
GP YN N NC N I ++SY+ +S+ + L
Sbjct: 589 GPIRYNMNSNCTPNSIASIIQKKSYNVSSITYEL 622
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 27.1 bits (57), Expect = 3.5
Identities = 19/62 (30%), Positives = 24/62 (38%), Gaps = 1/62 (1%)
Frame = +2
Query: 506 PKKNNFNGDKSPGNMQYGNKNDFG-GPKQQNYNKNYGPKTYNNQNCYGNEQPEFIPRQSY 682
PK N +N M + N GP Q N Y P + N N EF+ SY
Sbjct: 711 PKTNTYNSRHMAYEMTKSHINVISPGPSLQ-VNAPYTPTSGELGNKVSNPTKEFVSTSSY 769
Query: 683 SP 688
+P
Sbjct: 770 AP 771
>SPBP4H10.14c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 308
Score = 27.1 bits (57), Expect = 3.5
Identities = 15/56 (26%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
Frame = +2
Query: 350 PRPNMMNKNFRPRNDFNE--VKNDYNTKNDGNQNDFGGPKQFRPRNNFNNGNQPPK 511
P P+ + N F++ + Y+ +D N +DFGG NF N K
Sbjct: 80 PSPSSFLSDHNNNNLFSDDTISRQYSNTDDINPSDFGGQCAILDSQNFTLSNASTK 135
>SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 475
Score = 27.1 bits (57), Expect = 3.5
Identities = 22/88 (25%), Positives = 36/88 (40%), Gaps = 4/88 (4%)
Frame = +2
Query: 413 DYNTKNDG-NQNDFGGPKQFRPRNNFNNGNQPPKKNNFNGDKSPGNMQYGNKNDFGGPKQ 589
D +T N N F P Q P N+F+N N N+FN ++ ++ +G Q
Sbjct: 165 DKSTSNSTVTSNQFNKPTQNSPFNSFSNNN-----NSFNNNQQANDI-FGAPTTSAFTSQ 218
Query: 590 QN---YNKNYGPKTYNNQNCYGNEQPEF 664
N +++N ++ N N F
Sbjct: 219 LNASPFSQNTSSNSFTGSNPVQNNPSSF 246
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 26.6 bits (56), Expect = 4.6
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +1
Query: 175 STVKPFEANKSIVHHQNAVQRVSQSTSDFQASARPQ*TIR*NSI 306
S + PF +KS HH ++ +V +STS+ + S P T+ N +
Sbjct: 534 SPISPFSKSKSHNHHPSS--QVEKSTSNSKGSMLPLDTLYNNKL 575
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 26.6 bits (56), Expect = 4.6
Identities = 11/44 (25%), Positives = 23/44 (52%)
Frame = +3
Query: 645 GMSSLNLFLGNLTVQTLFNIL*MIANYRAKKQNILVIAC*TXVG 776
G++ + +LG + + ++ I N A + +I +I+C VG
Sbjct: 264 GIARIQFYLGKFDISLIMALISAIINKTASQNSIKIISCLYQVG 307
>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 991
Score = 26.6 bits (56), Expect = 4.6
Identities = 32/122 (26%), Positives = 52/122 (42%), Gaps = 19/122 (15%)
Frame = +2
Query: 338 KNFGPRPNMMNKNFRPRNDFNEVKNDYNTKNDGNQNDFGGPKQFRPRNNFNNGN-QPPKK 514
++F R N N ++R + V+ Y +++ ++ + G N F NG QPP
Sbjct: 874 RSFSSRRNNGN-SYRGGHQSYGVRRSYQSQSYSSRQSYTGVT-----NGFANGGVQPPWS 927
Query: 515 NNFNGDKS-------PGNMQYGNKNDFGG-----PKQQNYNKN------YGPKTYNNQNC 640
N N +S G+ YG ++ GG P +Y+ N Y ++YNN N
Sbjct: 928 GNGNFPRSNASYNSRGGHEGYGGRSRGGGYSNGPPAGNHYSSNRGKGYGYQRESYNNNNR 987
Query: 641 YG 646
G
Sbjct: 988 NG 989
>SPAC4H3.12c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 101
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = -3
Query: 628 VVCFRPIILVIILLFRTAKIIF 563
V CF PI+L IIL T IF
Sbjct: 22 VSCFNPILLKIILFLNTIVCIF 43
>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 639
Score = 26.2 bits (55), Expect = 6.1
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 597 ITRIMGLKHTTTRIAMGMSSLNLFLGNLTVQTLF 698
IT++ L +TTR A G + NLF G ++ T F
Sbjct: 474 ITKMTMLASSTTRPATGQTGDNLFRGFSSLSTRF 507
>SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 703
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/58 (22%), Positives = 26/58 (44%)
Frame = +3
Query: 495 VTNHQRKTTLMETNHQEICNMAIKMILAVLNNKIITRIMGLKHTTTRIAMGMSSLNLF 668
+ HQRKT + N C M ++++ + +N + + L + G+ L L+
Sbjct: 598 IREHQRKTFCVGLNWSYTCEMLLEIVDCINDNGLAQIFLALTQDYKNSSSGIPDLCLW 655
>SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1465
Score = 25.8 bits (54), Expect = 8.1
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = +3
Query: 45 FALCAIRQRAAIVELFVCGVWXGSYVNDHTLK 140
F CA +++ +CG+ G Y++D LK
Sbjct: 954 FLSCAFISSSSLTMTVLCGIRSGRYLHDSMLK 985
>SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 381
Score = 25.8 bits (54), Expect = 8.1
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = -3
Query: 280 AVWLRPENRMLIVKPVVLHFDGG 212
+VWL N M P++LH GG
Sbjct: 105 SVWLAKVNGMTKSDPIILHLHGG 127
>SPAC664.08c |||traub transciption factor family protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 452
Score = 25.8 bits (54), Expect = 8.1
Identities = 24/93 (25%), Positives = 37/93 (39%)
Frame = +2
Query: 389 NDFNEVKNDYNTKNDGNQNDFGGPKQFRPRNNFNNGNQPPKKNNFNGDKSPGNMQYGNKN 568
+ NE KND TK G Q N F NQ + N + ++ +
Sbjct: 260 DSLNEWKNDTLTKWHNRVQAVQGISQ---SNKFKALNQSIVQQIENSMINKKDLVERTRI 316
Query: 569 DFGGPKQQNYNKNYGPKTYNNQNCYGNEQPEFI 667
D+ P NK + P+ YN+ + Y + +FI
Sbjct: 317 DYSDPN----NKTFNPEIYNDTDFYQSLLKDFI 345
>SPAC17C9.12 |||MSP domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 25.8 bits (54), Expect = 8.1
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +2
Query: 350 PRPNMMNKNFRPRNDFNEVKNDYNTKNDGNQNDFGGPKQFRPRNNFNNGNQ 502
P+P + P N+ N +++ N N+ Q+ P F N+GNQ
Sbjct: 227 PKPQSVPTTTSPNNENNALRSTANVINNTRQSTATSPSMFAG----NSGNQ 273
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,289,254
Number of Sequences: 5004
Number of extensions: 72468
Number of successful extensions: 271
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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