BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP25_F_A03
(950 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 31 0.24
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 0.28
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 28 1.7
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.9
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 23 4.7
SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar... 27 5.1
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 23 6.5
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 31.1 bits (67), Expect = 0.24
Identities = 17/50 (34%), Positives = 18/50 (36%)
Frame = +2
Query: 413 GXXGGXGGGGXGGXXEXGX*GKGGXRGXGGGXGXXXXKXGXXGXXXMGGK 562
G GG GGG G +GG G GG G G G G K
Sbjct: 23 GGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGGAK 72
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 24.6 bits (51), Expect(2) = 0.28
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = -3
Query: 465 PXSXXPPXPPPPXP 424
P PP PPPP P
Sbjct: 184 PSDYNPPPPPPPPP 197
Score = 24.6 bits (51), Expect(2) = 0.28
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -3
Query: 447 PXPPPPXPPFXP 412
P PPPP PP P
Sbjct: 236 PAPPPPPPPTLP 247
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 28.3 bits (60), Expect = 1.7
Identities = 15/30 (50%), Positives = 15/30 (50%)
Frame = +2
Query: 422 GGXGGGGXGGXXEXGX*GKGGXRGXGGGXG 511
GG GG GG G G GG G GGG G
Sbjct: 446 GGGSRGGRGGFGGRG--GFGGRGGFGGGRG 473
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 3.9
Identities = 18/48 (37%), Positives = 19/48 (39%), Gaps = 5/48 (10%)
Frame = +2
Query: 413 GXXGGXGG-----GGXGGXXEXGX*GKGGXRGXGGGXGXXXXKXGXXG 541
G GG GG GG GG +GG G GGG G G G
Sbjct: 208 GGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFG 255
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 23.0 bits (47), Expect(2) = 4.7
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -3
Query: 450 PPXPPPPXP 424
PP PPPP P
Sbjct: 775 PPPPPPPPP 783
Score = 21.8 bits (44), Expect(2) = 4.7
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = -3
Query: 465 PXSXXPPXPPPP 430
P PP PPPP
Sbjct: 756 PIMGGPPPPPPP 767
>SPBP8B7.16c |dbp2||ATP-dependent RNA helicase
Dbp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 5.1
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = +2
Query: 437 GGXGGXXEXGX*GKGGXRGXGGGXG 511
GG GG G G+GG R GGG G
Sbjct: 508 GGRGGNYRRGGYGRGGFR-RGGGYG 531
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 22.6 bits (46), Expect(2) = 6.5
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = -2
Query: 439 PPPXPPXXPXFFXSPXSXP 383
PPP PP SP S P
Sbjct: 370 PPPPPPPPELLNHSPKSRP 388
Score = 21.8 bits (44), Expect(2) = 6.5
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = -2
Query: 589 PFXPFFXXXFSPHXXLPLXPPFXXXXPXSXPXPP 488
P PFF FSPH S P PP
Sbjct: 341 PPAPFFPLGFSPHLNDESSRSRWSNISYSPPPPP 374
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,099,913
Number of Sequences: 5004
Number of extensions: 9533
Number of successful extensions: 108
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 75
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 485316198
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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