Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_N21
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 35 0.013
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 34 0.023
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 29 0.88
SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi... 27 3.6
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.6
SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase Cgs2|Schi... 27 3.6
SPAC1F12.09 |gpi17||pig-S|Schizosaccharomyces pombe|chr 1|||Manual 27 3.6
SPBC11B10.01 |alg2|SPBC32H8.14|mannosyltransferase complex subun... 27 3.6
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 27 4.7
SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pomb... 26 6.2
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 6.2
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 26 6.2
SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr 3... 26 8.2
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 35.1 bits (77), Expect = 0.013
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +3
Query: 417 VSLPSLIGKPPPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAK 572
V L S+ PP + + P + +K+DPL +I +A P P + G T+K
Sbjct: 1935 VPLSSMPNAPPSVASNAKLPPASNNRKVDPLEDILQAMPPPTTRKARGKTSK 1986
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 34.3 bits (75), Expect = 0.023
Identities = 35/151 (23%), Positives = 52/151 (34%), Gaps = 8/151 (5%)
Frame = +3
Query: 408 PAVVSLPSLIGKPPPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAKAPNTY 587
P +PS+ +PP AP+ T A++ P + + + AP
Sbjct: 1493 PVPSMIPSVAQQPPSSVAPATAPSSTLPPSQSSFAHVPSPAPPAPQHPSAAALSSAPADN 1552
Query: 588 SMP----PVLGEAKEGSKRA----APAFSITGRGKFSEAKGLMPGPGTYTTDRAAAALTK 743
SMP P + +A APA ++ FS G + G+ AA +
Sbjct: 1553 SMPHRSSPYAPQEPVQKPQAINNIAPATNLGTSQSFSPRMGPVNNSGSPLAMNAAGQPSL 1612
Query: 744 RPPAFTMAPRRELKPPTAAVPGSRSVLS*EG 836
PA AP P P + S L G
Sbjct: 1613 AVPAVPSAPSNHFNPFAKMQPPAPSPLQPSG 1643
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 29.1 bits (62), Expect = 0.88
Identities = 40/159 (25%), Positives = 60/159 (37%), Gaps = 8/159 (5%)
Frame = +3
Query: 348 NPWTPTKRRGPIAAEA---SSPG--PAVVSLPSLIGKP---PPESRKPRAPAFTFGQKLD 503
NP+TP + P+AA + SSP P+ P + P + KP +
Sbjct: 955 NPYTPVAPQSPVAAASRISSSPNMPPSNPYTPIAVASSTVNPAHTYKPHGGSQIVPPPKQ 1014
Query: 504 PLANIAKAGPGPASYNTEGMTAKAPNTYSMPPVLGEAKEGSKRAAPAFSITGRGKFSEAK 683
P AN P AS +A P T S+P + + + P S S +
Sbjct: 1015 P-ANRVVPLPPTASQRA---SAYEPPTVSVPSPSALSPSVTPQLPPVSSRLP--PVSATR 1068
Query: 684 GLMPGPGTYTTDRAAAALTKRPPAFTMAPRRELKPPTAA 800
+P P +T +++ RPP T A R T+A
Sbjct: 1069 PQIPQPPPVSTALPSSSAVSRPPIATSAGRSSTAASTSA 1107
>SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase
Gut2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 649
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = -2
Query: 697 PGINPLASENFPRPVILKAGAALLLPSFASPSTGGI 590
P P + FPR V+ AG ++LP + P GI
Sbjct: 297 PQGEPPKTAQFPRMVVPSAGVHVVLPEYYCPPNIGI 332
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.1 bits (57), Expect = 3.6
Identities = 29/140 (20%), Positives = 46/140 (32%), Gaps = 5/140 (3%)
Frame = +3
Query: 402 PGPAVVSLPSLIGKPPPESRKPRAPAFTF---GQKLDPLANIAKAGPGPASYNTEGMTAK 572
P P P+ PP R+ G+KL ++ P +
Sbjct: 6 PPPPPAPAPAAAAPAPPLMTGDRSALLNSIQKGKKLKKATTNDRSAPVVGGGVVGERKSN 65
Query: 573 APNTYSMPPVLGEAKEGSKRAAPAFSITGRGKFSEAKGLMPGPGTYTTDRAAAALTKRPP 752
P +++ PPV A + R GL G ++ + PP
Sbjct: 66 TPKSFAAPPVPTGAPSLPTSSNNTQQAEERPSMPALGGLFAGGMPKLRHIGKSSASAAPP 125
Query: 753 AFTM--APRRELKPPTAAVP 806
+ P+ EL+PPT+A P
Sbjct: 126 SAPAPPTPQSELRPPTSAPP 145
>SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase
Cgs2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 346
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +2
Query: 407 TSRSISPFPYR*TASRIEETKSSSFYFR 490
T+ SI PFP +S +E KSS+F FR
Sbjct: 182 TTLSILPFPVNHGSSFGQELKSSAFLFR 209
>SPAC1F12.09 |gpi17||pig-S|Schizosaccharomyces pombe|chr 1|||Manual
Length = 554
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = -2
Query: 715 VVYVPGPGINPLASENFPRPVILKAGAALLLPSFASPST 599
V+YVP P I PL EN +I ++LLP + S +T
Sbjct: 335 VLYVPSPQIQPLWLENEDSNII--PTNSMLLPQWGSITT 371
>SPBC11B10.01 |alg2|SPBC32H8.14|mannosyltransferase complex subunit
Alg2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 511
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
Frame = +1
Query: 565 LLKHPILILCH---RYLAKRKKGVKELHRLSVSQVVESFLKLKD 687
L IL CH +YLAKR +K+L+R+ V ++L D
Sbjct: 127 LASQMILFYCHFPDKYLAKRGGILKKLYRIPFDTVEAESVRLAD 170
>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 786
Score = 26.6 bits (56), Expect = 4.7
Identities = 28/128 (21%), Positives = 46/128 (35%)
Frame = +3
Query: 219 GFMNVDSCR*QVTADFRKSGLTHASSLLVKQSISCETPMGVRQNPWTPTKRRGPIAAEAS 398
GF+ + S R + FR + + L K S + + +G P+K R P +S
Sbjct: 84 GFLGLVSFRMREVGSFRSNREVSLTRPLKKSSTTNLSVLGNLVLKVAPSKIRAPAGNHSS 143
Query: 399 SPGPAVVSLPSLIGKPPPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAKAP 578
+ S P+ +R P T +N + G A+ N G A
Sbjct: 144 TTANRTTSTPTT--TTARTTRTTPRPTATTNTSNQSTSNSTRNGTSAATSNGTGTGAGTG 201
Query: 579 NTYSMPPV 602
++ PV
Sbjct: 202 ASHRSSPV 209
>SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 301
Score = 26.2 bits (55), Expect = 6.2
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 2/85 (2%)
Frame = +3
Query: 327 TPMGVRQNPWTPTKRRGPIAAEASSPGPAVVSLPSLI--GKPPPESRKPRAPAFTFGQKL 500
TP+ R +R P+ + S P+ V++P + GK S+ AP+ +K+
Sbjct: 18 TPISNRNGTKGAGSKRAPLGSTKQSNAPSSVTVPRTVLGGKSTNISKFISAPS---TKKM 74
Query: 501 DPLANIAKAGPGPASYNTEGMTAKA 575
P+ +I+ P N++G++ A
Sbjct: 75 SPM-DISMDSPTILEPNSQGISRSA 98
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 6.2
Identities = 30/119 (25%), Positives = 45/119 (37%), Gaps = 1/119 (0%)
Frame = +3
Query: 438 GKPPPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAKAPNTYSMPPVLGEAK 617
G P S P P+ + + + +++ P P + G + PN S P +
Sbjct: 222 GTPTSTSAPPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPN--SPPRPIAPVS 279
Query: 618 EGSKRAAPAFSITGRGKFSEAKGLMPGPGTYTTDRAAAALTKRPPAFTMAPRREL-KPP 791
PA + T +K +P P + + A AA KRPP RR KPP
Sbjct: 280 MN-----PAINST-------SKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPP 326
Score = 25.8 bits (54), Expect = 8.2
Identities = 18/56 (32%), Positives = 22/56 (39%), Gaps = 1/56 (1%)
Frame = +3
Query: 330 PMGVRQNPWTPTKRRGPIAAEASSPGPAVVSLPSLIGKPPP-ESRKPRAPAFTFGQ 494
PMG P P PIA + PA LP PPP + P AP + +
Sbjct: 440 PMGAPAAP--PLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVASIAE 493
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 26.2 bits (55), Expect = 6.2
Identities = 9/23 (39%), Positives = 17/23 (73%)
Frame = +1
Query: 595 HRYLAKRKKGVKELHRLSVSQVV 663
+++L +KG +ELHR +SQ++
Sbjct: 216 YQFLKDMRKGFRELHRKDLSQLI 238
>SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 334
Score = 25.8 bits (54), Expect = 8.2
Identities = 18/79 (22%), Positives = 37/79 (46%)
Frame = +3
Query: 318 SCETPMGVRQNPWTPTKRRGPIAAEASSPGPAVVSLPSLIGKPPPESRKPRAPAFTFGQK 497
S E ++ NP + +K + + + GP ++S P +I P + + + +K
Sbjct: 241 SSEMDSNLQTNPSSISKGKKNNSNNTTQKGPNIISSPQVINASSPVRKAGKKKS----KK 296
Query: 498 LDPLANIAKAGPGPASYNT 554
P + ++ + P ASYN+
Sbjct: 297 ALPTSPLSSSSP-DASYNS 314
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,589,841
Number of Sequences: 5004
Number of extensions: 77814
Number of successful extensions: 255
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 254
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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