Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_M12
(878 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 33 0.041
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 31 0.22
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te... 31 0.29
SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccha... 29 0.87
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.7
SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizo... 27 2.7
SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.5
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 27 3.5
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.5
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 6.1
SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa... 26 6.1
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 33.5 bits (73), Expect = 0.041
Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +3
Query: 123 MLAASAGVV--ELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQGKGSII 278
++ A+AG+ LS + N+D+ K+ L G Y +A ++ QGKGS+I
Sbjct: 91 VMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLI 144
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 31.1 bits (67), Expect = 0.22
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = +1
Query: 586 HNTKYNQYLKMSTTTCNCNSRDRVVYGGNSADSTRE 693
+++ YN+ M T++C+C+S + YGGN A E
Sbjct: 47 YSSTYNEITNMDTSSCSCSSTPK-SYGGNLAPFDEE 81
>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
termination factor Reb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 504
Score = 30.7 bits (66), Expect = 0.29
Identities = 13/59 (22%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +3
Query: 270 SIIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVRKYFPLNF--XTHHGRKLCQDHLQK 440
+II V+N I+D+ + ++C ++W G + +R ++ + +H K H+++
Sbjct: 247 AIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRR 305
>SPAC4F8.11 |||WD repeat protein, human WDR24
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 846
Score = 29.1 bits (62), Expect = 0.87
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +1
Query: 586 HNTKYNQYLKMSTTTCNCNSRDRVVYGGNSA 678
HN +N + + S T+ + SR+ V+ GNS+
Sbjct: 617 HNEMFNSFHRSSVTSASIKSREAVLSAGNSS 647
>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 27.5 bits (58), Expect = 2.7
Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +1
Query: 646 RDRVVYGGNSADSTREQWFFQPAKYENDVLFFIYNRQ--FNDALELGTIVNASGDRKAVG 819
R R+V G ++A + + W F +Y ++F+++N F AL T V GD A
Sbjct: 162 RQRIVVGKHAAHFSLDHWIF-VVEYYAPIVFYVFNLMGFFLAALHPWTKV-VRGDGNA-A 218
Query: 820 HDGEVAGSS 846
DG SS
Sbjct: 219 TDGRFKASS 227
>SPBC800.09 |sum2||G2/M transition checkpoint protein
Sum2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -1
Query: 845 EDPATSPSCPTALRSPEAFTIVPSSKASLNW 753
E+PAT+PS P ++ P I+ S+ NW
Sbjct: 77 EEPATTPSAP-PVQPPNDPAIIGSNSGQYNW 106
>SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 300
Score = 27.1 bits (57), Expect = 3.5
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -1
Query: 248 FQALTDSTVVVAGEDAVVQFLLEVLV 171
F +T V+V EDAVV+F+L +LV
Sbjct: 181 FLGVTVQYVMVLPEDAVVEFVLTILV 206
>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
2|||Manual
Length = 918
Score = 27.1 bits (57), Expect = 3.5
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 5/42 (11%)
Frame = -1
Query: 386 WEVLSNNFLS--VADPQL---VAVLHGVPSLVNDQVVNYILD 276
W+VL +++L+ ++ P V LHGV + VN V +YI D
Sbjct: 188 WDVLFHDYLNETLSQPAFSFNVPDLHGVDNKVNQYVFDYIKD 229
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +3
Query: 171 NQDLEEKLYNSILTGDYDSAVRQSLE 248
+ DLE+ + ++LTGD SAV+ LE
Sbjct: 551 DSDLEKNITEALLTGDVLSAVKACLE 576
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +1
Query: 136 ARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRAW 246
AR SLNY ++ + SRRN ++P S + W
Sbjct: 622 ARESLNYLKSFNKQLSRRNAPDINNPIADFQNSFQNW 658
>SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 889
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 273 IIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVR 374
+++++ NL I N +EY LW NG I +
Sbjct: 447 VLKDIFFNLQIGVTFNILEYLRHLWSNNGDAIAK 480
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,246,988
Number of Sequences: 5004
Number of extensions: 64358
Number of successful extensions: 230
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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