Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_M11
(881 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 316 3e-87
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 315 5e-87
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 27 2.7
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 27 4.7
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 4.7
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 26 8.2
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 26 8.2
>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 335
Score = 316 bits (776), Expect = 3e-87
Identities = 157/236 (66%), Positives = 178/236 (75%), Gaps = 1/236 (0%)
Frame = +2
Query: 134 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 310
K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE +
Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64
Query: 311 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 490
G LV++G+ I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124
Query: 491 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 670
S DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTAT 184
Query: 671 XXXXDGPSGKLWRDGRGAQQNIIPASTXXXXXXXXXXXXLNGKLTGMAFRVPVANV 838
DGPS K WR GRGA NIIP+ST LNGKLTGMAFRVP +V
Sbjct: 185 QKTVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDV 240
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 315 bits (774), Expect = 5e-87
Identities = 156/236 (66%), Positives = 178/236 (75%), Gaps = 1/236 (0%)
Frame = +2
Query: 134 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 310
K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64
Query: 311 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 490
G LV++GN I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124
Query: 491 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 670
S DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTAT 184
Query: 671 XXXXDGPSGKLWRDGRGAQQNIIPASTXXXXXXXXXXXXLNGKLTGMAFRVPVANV 838
DGPS K WR GRGA NIIP+ST LNGKLTGMAFRVP +V
Sbjct: 185 QKTVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDV 240
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = -3
Query: 192 SIEARSTKRPMRPNPLIPIFDMLSASYRKFVE*LTTTTYAQVQDG 58
++ A+ TK P+RP + P + SA + ++ + T AQVQ+G
Sbjct: 179 ALAAKLTK-PIRPLWVTPASRLTSAQFEEYFTVVLVTASAQVQNG 222
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 26.6 bits (56), Expect = 4.7
Identities = 21/68 (30%), Positives = 28/68 (41%)
Frame = -2
Query: 868 PRRTVRSTTGYISNRDAECHSSQLAIKSXDNLTHSFGSTSRGRNDVLLSTTAITP*FSRR 689
P R R + RD HS+ I S D+ + + S S N V T +TP R
Sbjct: 278 PERPRRERLHAMRVRDIRSHSTNTTITSVDSASTALNSYST-PNSVSRKLTNLTPAARRL 336
Query: 688 SINSFLCS 665
S+L S
Sbjct: 337 VARSYLRS 344
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 26.6 bits (56), Expect = 4.7
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = -2
Query: 340 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 185
+F + N+ESIL+L L + + E I++IV+ +D R HLS L +
Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392
>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/62 (24%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = -2
Query: 499 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPWNGLRVPF*E-NGNFVTVN 323
+ +G +N +S + C +C G + ++ +P F +R+P NGN T+
Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGFR--QQMRMPCNACNGNGRTIK 210
Query: 322 NK 317
+K
Sbjct: 211 HK 212
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 25.8 bits (54), Expect = 8.2
Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 1/75 (1%)
Frame = -2
Query: 859 TVRSTTGYIS-NRDAECHSSQLAIKSXDNLTHSFGSTSRGRNDVLLSTTAITP*FSRRSI 683
TV T G S +R S+ A+ + L S + + S T++ P R+
Sbjct: 52 TVAGTEGGSSLSRRHTSAESRKALPNQQQLAQSGLLNKEEQQSLKRSDTSVFPKAVRKVS 111
Query: 682 NSFLCSSCGMNSSHQ 638
+S +C+SCG S Q
Sbjct: 112 SSKICASCGQVISGQ 126
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,561,617
Number of Sequences: 5004
Number of extensions: 75503
Number of successful extensions: 235
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 233
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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