Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_M02
(891 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 413 e-118
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 406 e-115
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 295 3e-82
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 262 3e-72
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.9
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 413 bits (1018), Expect = e-118
Identities = 197/206 (95%), Positives = 198/206 (96%)
Frame = +1
Query: 115 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 294
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 295 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGT 474
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 475 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKK 654
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEI KEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 655 IGYXPXAVAFVPISGWHGXNMLEPST 732
IGY P AVAFVPISGWHG NMLE S+
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSS 206
Score = 47.6 bits (108), Expect = 2e-07
Identities = 20/29 (68%), Positives = 21/29 (72%)
Frame = +2
Query: 737 MPWFKGWXVXRKEGXXDGXCPIEXLXAIL 823
MPWFKGW V RKEG +G C IE L AIL
Sbjct: 208 MPWFKGWTVERKEGKVEGKCLIEALDAIL 236
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 406 bits (999), Expect = e-115
Identities = 193/213 (90%), Positives = 197/213 (92%)
Frame = +1
Query: 115 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 294
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 295 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGT 474
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 475 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKK 654
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEI KEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 655 IGYXPXAVAFVPISGWHGXNMLEPSTXNALVQG 753
IGY +VAFVPISGWHG NMLEPS +G
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKG 213
Score = 42.7 bits (96), Expect = 4e-06
Identities = 18/31 (58%), Positives = 20/31 (64%)
Frame = +2
Query: 731 PXMPWFKGWXVXRKEGXXDGXCPIEXLXAIL 823
P PW+KGW V RK+G DG IE L AIL
Sbjct: 206 PKTPWYKGWKVERKDGNADGKTLIEALDAIL 236
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 295 bits (724), Expect = 3e-82
Identities = 141/149 (94%), Positives = 142/149 (95%)
Frame = +1
Query: 286 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVX 465
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 466 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSY 645
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEI KEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 646 IKKIGYXPXAVAFVPISGWHGXNMLEPST 732
IKKIGY P AVAFVPISGWHG NMLE S+
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSS 149
Score = 47.6 bits (108), Expect = 2e-07
Identities = 20/29 (68%), Positives = 21/29 (72%)
Frame = +2
Query: 737 MPWFKGWXVXRKEGXXDGXCPIEXLXAIL 823
MPWFKGW V RKEG +G C IE L AIL
Sbjct: 151 MPWFKGWTVERKEGKVEGKCLIEALDAIL 179
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 262 bits (642), Expect = 3e-72
Identities = 125/133 (93%), Positives = 126/133 (94%)
Frame = +1
Query: 334 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGTGEFEAGISKNGQT 513
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 514 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKKIGYXPXAVAFVPI 693
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEI KEVSSYIKKIGY P AVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 694 SGWHGXNMLEPST 732
SGWHG NMLE S+
Sbjct: 121 SGWHGDNMLEVSS 133
Score = 47.6 bits (108), Expect = 2e-07
Identities = 20/29 (68%), Positives = 21/29 (72%)
Frame = +2
Query: 737 MPWFKGWXVXRKEGXXDGXCPIEXLXAIL 823
MPWFKGW V RKEG +G C IE L AIL
Sbjct: 135 MPWFKGWTVERKEGKVEGKCLIEALDAIL 163
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.5 bits (73), Expect = 0.003
Identities = 35/117 (29%), Positives = 51/117 (43%)
Frame = +1
Query: 373 VTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGTGEFEAGISKNGQTREHALLAFTLGVK 552
VT +D PGH FI G D VL+V A G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 553 QLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKKIGYXPXAVAFVPISGWHGXNMLE 723
+IV +NK+D + ++ E+ K I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQY-ELAKH-GIVIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.70
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 127 KTHINIVVIGHVDSGKST 180
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 136 INIVVIGHVDSGKST 180
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.7
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -2
Query: 128 FSLPIFG*SRITNCV*Y 78
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 358 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 459
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 358 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 459
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 208,914
Number of Sequences: 438
Number of extensions: 3849
Number of successful extensions: 18
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 58
effective length of database: 120,939
effective search space used: 28783482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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