Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_H16
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 240 2e-64
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 226 4e-60
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 58 2e-09
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 29 0.66
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.87
SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch... 27 3.5
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 27 3.5
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 27 4.7
SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|ch... 27 4.7
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 26 6.1
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch... 26 8.1
SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase |Schizosacch... 26 8.1
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 240 bits (588), Expect = 2e-64
Identities = 116/231 (50%), Positives = 160/231 (69%), Gaps = 1/231 (0%)
Frame = +1
Query: 190 FEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKITSLNESPS 369
F L I+QVGGREDSNVY+RMK +AA + GI+ EH+ P DITE +LL I NE P+
Sbjct: 32 FNVSLKIIQVGGREDSNVYVRMKTRAANEAGISCEHVNFPEDITEYDLLLAIKGFNEDPT 91
Query: 370 VHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTINEGRVAVGDLSG-FIPCTPAGCV 546
VHGIIVQ+PL + I+ +T+AV+P+KDVDG N G++ + F CTP G +
Sbjct: 92 VHGIIVQLPLPAH--INEQIITEAVAPEKDVDGFCETNLGKLTKREGQPLFTACTPKGIM 149
Query: 547 ELIKKTGVTIAGKNVVVLGRSRIVGTPVSELLKWEHATVTVCHSKTKNLSEITKTAEILV 726
++K G+ + GK+ VV+GRS IVG P+S LL+ +ATVT+CHSKT+++++I +TA+I+V
Sbjct: 150 CILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTLCHSKTESIADIVRTADIVV 209
Query: 727 VAIGRPEMVRGSWIKPGAVVIDCGINAIEDPTKKSGQRXVGDVAYQXAXKV 879
AIG P V+ W+K G V ID GIN+I D TKKSG R GD+ ++ A +V
Sbjct: 210 AAIGIPHFVKADWLKKGVVAIDVGINSIPDATKKSGYRLTGDIDFENAKEV 260
>SPBC2G2.08 |ade9||C-1-
tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 969
Score = 226 bits (552), Expect = 4e-60
Identities = 114/237 (48%), Positives = 162/237 (68%), Gaps = 2/237 (0%)
Frame = +1
Query: 175 SKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKITSL 354
+K F+P LAIVQVG REDSNVY+RMK KAA +GI ++ P I LL ++ L
Sbjct: 58 AKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLVGIDFKYCPFPETIQMPALLHELKKL 117
Query: 355 NESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTINEGRVAVGDLSGF-IPCT 531
N+ +VHG++VQ+PL ++ VT++++P KDVDG N G +A D + PCT
Sbjct: 118 NDDHTVHGVLVQLPLPKH--LNERTVTESITPPKDVDGFGAFNIGLLAKNDATPIHYPCT 175
Query: 532 PAGCVELIKKTGVTIAGKNVVVLGRSRIVGTPVSELLKWEHATVTVCHSKTKNLSEITKT 711
P G +EL+K +++AG N VVLGRS IVG P+S LL+ ++ATVTVCHSKTK+L +
Sbjct: 176 PKGIMELLKDNKISVAGLNAVVLGRSDIVGNPISYLLRKDNATVTVCHSKTKDLIQHISN 235
Query: 712 AEILVVAIGRPEMVRGSWIKPGAVVIDCGINAIEDPTKKSGQR-XVGDVAYQXAXKV 879
A++++ A+G+PE VRG W+KPG+VV+D GINA++ ++G+R VGDV ++ A KV
Sbjct: 236 ADLVIAALGKPEFVRGEWLKPGSVVVDVGINAVQ----RNGKRVLVGDVHFESASKV 288
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 58.0 bits (134), Expect = 2e-09
Identities = 51/175 (29%), Positives = 81/175 (46%), Gaps = 17/175 (9%)
Frame = +1
Query: 190 FEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKITSLNESPS 369
F P+L + +Y K +IG E +P+D +L I N PS
Sbjct: 38 FAPKLVGFLSNSDPAARMYADWTNKTCTEIGFQYELREVPKD----DLEDAIVEANNDPS 93
Query: 370 VHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGL------NTINEGRVAVGDLS--GFIP 525
V+GI++ P+ +D D + + VSPDKDV+GL N + R + + +P
Sbjct: 94 VNGIMIYFPVFNDGQ-DQY-LQQVVSPDKDVEGLCHKYVMNMYHNIRHLDPEKTKKSILP 151
Query: 526 CTPAGCVELIKKTGV---------TIAGKNVVVLGRSRIVGTPVSELLKWEHATV 663
CTP V++++ GV + GK + ++ RS IVG P++ LL + A V
Sbjct: 152 CTPLAIVKILEYLGVYNKIINYGNRLYGKTITIVNRSEIVGRPLAALLANDGAKV 206
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 29.5 bits (63), Expect = 0.66
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
Frame = -3
Query: 620 PTMRLRPSTTTFL-PAIVTPVFLMSSTHPAGVQGINPDRSPTATRPSLIVFKPSTS 456
P P+ +TF P++ +P F SST NP +P + P KPST+
Sbjct: 366 PLQNTNPAPSTFPNPSVASPAFPNSSTS-------NPSTAPASASPLASTLKPSTA 414
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.1 bits (62), Expect = 0.87
Identities = 29/109 (26%), Positives = 48/109 (44%), Gaps = 1/109 (0%)
Frame = -3
Query: 758 PRTISGRPIAT-TSISAVLVISLKFLVFEWHTVTVACSHFSNSDTGVPTMRLRPSTTTFL 582
P T + R T TS +++ S + + S +SN T T+ P TT+F
Sbjct: 640 PLTSTNRTSTTFTSSTSISTSSSSTATSSTSFASESSSFYSNVTTSSSTVSTPPPTTSF- 698
Query: 581 PAIVTPVFLMSSTHPAGVQGINPDRSPTATRPSLIVFKPSTSLSGETAS 435
P+ T F+ SS+ + N TA+ S K +++ SG ++S
Sbjct: 699 PSTFTTSFITSSSLSSIPN--NSTEVKTASTSSGTEIKTASTSSGSSSS 745
>SPAC14C4.08 |mug5||meiotically upregulated gene
Mug5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 179
Score = 27.1 bits (57), Expect = 3.5
Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +1
Query: 184 SGFEPRLAIVQVGGRE-DSNVYIRMKLKAAEKI 279
SGFE R+ I++ E DSN+YIR+K + K+
Sbjct: 11 SGFENRIKILEDLMNEFDSNLYIRLKRECDFKL 43
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 27.1 bits (57), Expect = 3.5
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -2
Query: 399 KGHLNNDAVNRRRFIQTRYF 340
KG+LNN A N+ F + R+F
Sbjct: 365 KGYLNNPAANKSSFTKDRFF 384
>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
1|||Manual
Length = 927
Score = 26.6 bits (56), Expect = 4.7
Identities = 23/95 (24%), Positives = 40/95 (42%), Gaps = 8/95 (8%)
Frame = -2
Query: 444 DSISDSMSVDRVVRIKGHLNNDAVNR------RRFIQTRYFC*QLYFSDVPW*--ADMFG 289
D+ S + +VR++ N + V R + YFC + W A F
Sbjct: 701 DASSSAAFNASMVRLRSTFNEELVELFENMAVRSLNRAEYFCTGDFGEKTDWHHYALSFN 760
Query: 288 GDTNFLSCLQLHPDVDVRVLAPAHLHDGKPGLEAR 184
T+F S ++ +PD+ V L L + PG++ +
Sbjct: 761 HYTHFTSPIRRYPDIIVHRLLERSLKNTSPGIDKK 795
>SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 979
Score = 26.6 bits (56), Expect = 4.7
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +2
Query: 59 IGSTNETVLWAKMAQIISWD*SC 127
I N+T+LW + +I WD C
Sbjct: 42 ISILNDTLLWNRFINVIEWDKLC 64
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +2
Query: 758 ALGSSLEPSL*TVVSTLLRILQRKAGNV 841
ALG+ +P L +VST+L L K+ NV
Sbjct: 804 ALGTRCKPYLPQIVSTILYRLNNKSANV 831
>SPBC1289.02c |uap2||U2 snRNP-associated protein
Uap2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 367
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +1
Query: 322 EIELLAKITSLNESPSVHGIIVQMPLD-SDHAIDAHRVTDAVSPDKDVDGLNTI 480
+I +L I +L E +++ + D ++ A RVT+ V DK+ DG+ T+
Sbjct: 249 KIVVLKHIFTLEELDKTPELLIDLKDDITEEAEKCGRVTNVVLYDKEPDGVVTV 302
>SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = +1
Query: 244 YIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKI 345
YI KL A G+A + I DI E+E+LAKI
Sbjct: 23 YISPKLYIASS-GVAGDGIFSTFDIDELEVLAKI 55
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,438,820
Number of Sequences: 5004
Number of extensions: 69676
Number of successful extensions: 184
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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