Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_H05
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 88 1e-18
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 73 7e-14
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 68 2e-12
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 56 7e-09
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 53 6e-08
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 49 1e-06
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 38 0.002
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 37 0.004
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 28 1.6
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 28 2.1
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 28 2.1
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 3.6
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 6.3
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 26 8.3
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 26 8.3
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 88.2 bits (209), Expect = 1e-18
Identities = 38/77 (49%), Positives = 52/77 (67%)
Frame = +2
Query: 188 LSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQ 367
++K +I+ + ++V+FYAPWCGHCK+LAPEY AA +L E+ I L +VD T+E
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEG 84
Query: 368 DLAESYGVRGYPTLKFF 418
DL Y +RGYPTL F
Sbjct: 85 DLCSEYSIRGYPTLNVF 101
Score = 81.8 bits (193), Expect = 1e-16
Identities = 39/85 (45%), Positives = 61/85 (71%), Gaps = 1/85 (1%)
Frame = +2
Query: 167 TEENVLVLSKANFETVI-STTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 343
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 344 KVDATQEQDLAESYGVRGYPTLKFF 418
K+DAT E D+ S + G+PT+ FF
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFF 433
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 72.5 bits (170), Expect = 7e-14
Identities = 37/103 (35%), Positives = 57/103 (55%)
Frame = +2
Query: 110 VLIFTAIALLGLALGDEVPTEENVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPE 289
+L F AL L V +++ L T+ ++ + L+EFYA WCGHCKSLAP
Sbjct: 5 LLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKSLAPV 60
Query: 290 YAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF 418
Y + L E+ + + + K+DA D+A+ Y + G+PTL +F
Sbjct: 61 YEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWF 102
Score = 68.5 bits (160), Expect = 1e-12
Identities = 32/82 (39%), Positives = 50/82 (60%), Gaps = 1/82 (1%)
Frame = +2
Query: 176 NVLVLSKANFETVISTTEY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVD 352
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y + K+ + E +++ K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNVEIVKIN 199
Query: 353 ATQEQDLAESYGVRGYPTLKFF 418
A D+ + V +PT+KFF
Sbjct: 200 ADVFADIGRLHEVASFPTIKFF 221
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 68.1 bits (159), Expect = 2e-12
Identities = 34/100 (34%), Positives = 47/100 (47%)
Frame = +2
Query: 113 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEY 292
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 293 AKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLK 412
K A+ L P+ DA Q + + Y V+G+PT+K
Sbjct: 71 QKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIK 109
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 56.0 bits (129), Expect = 7e-09
Identities = 25/78 (32%), Positives = 46/78 (58%)
Frame = +2
Query: 185 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 364
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 365 QDLAESYGVRGYPTLKFF 418
+ +A GV+ PT FF
Sbjct: 64 RQIASGLGVKAMPTFVFF 81
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 52.8 bits (121), Expect = 6e-08
Identities = 29/82 (35%), Positives = 42/82 (51%)
Frame = +2
Query: 185 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 364
V S ++ T IS + +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 365 QDLAESYGVRGYPTLKFFXEWQ 430
D+A+ GV PT+ F + Q
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQ 99
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 48.8 bits (111), Expect = 1e-06
Identities = 21/74 (28%), Positives = 40/74 (54%)
Frame = +2
Query: 185 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 364
V + F++++ + ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 365 QDLAESYGVRGYPT 406
++A GV P+
Sbjct: 61 SEIAAEAGVHAMPS 74
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/59 (30%), Positives = 30/59 (50%)
Frame = +2
Query: 227 EYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYP 403
+ IL+ FYAPW CK + + + A++ K++A + D+AES+ V P
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 36.7 bits (81), Expect = 0.004
Identities = 23/79 (29%), Positives = 38/79 (48%), Gaps = 2/79 (2%)
Frame = +2
Query: 188 LSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAE--EESPIKLAKVDATQ 361
L+ + E+ +S + +++Y P CG CK L P + K E E S +VD ++
Sbjct: 31 LTDNDLESEVSKGTWF-IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 362 EQDLAESYGVRGYPTLKFF 418
E L+ +R PTL +
Sbjct: 90 E--LSSCANIRAVPTLYLY 106
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.3 bits (60), Expect = 1.6
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +3
Query: 369 ISPRATVYEDTRLSNSSXNGSPIDYSGGRQADDIISWLKKKTG 497
ISP Y+DT+L+N + +G G QADD+ +K G
Sbjct: 157 ISPFQGYYDDTKLTNQAYHGI------GYQADDVFQLERKPVG 193
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -3
Query: 613 RKVLALAELWSEKKPNTITVLASISSLACSADVTSTA 503
+K +L + W + + LASISS A SA + ST+
Sbjct: 673 KKAASLPQFWLSPRSHNTARLASISSFAKSAVMNSTS 709
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 27.9 bits (59), Expect = 2.1
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +2
Query: 266 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVR 394
+C L P +AA L + +SP L VDA + L S+GVR
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVR 223
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 27.1 bits (57), Expect = 3.6
Identities = 17/58 (29%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +3
Query: 432 PIDYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGF-FSDQSSARA 602
P DY ++ D + ++ G P++ ++ K ++++ TV VFG F Q ARA
Sbjct: 291 PWDYDFTKETYDGL-FISNGPGDPSLMDLVVDRVKRVLESKTVPVFGICFGHQIMARA 347
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 357 VASTFASLIGDSSSASFVAALAYSGARDLQW 265
V++ F SL+G S FVA + R L+W
Sbjct: 1039 VSNIFKSLVGSMSCVEFVAEARETINRSLEW 1069
>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 747
Score = 25.8 bits (54), Expect = 8.3
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -3
Query: 112 NTHCDIIGTFLL 77
NTHCDI+ +FLL
Sbjct: 275 NTHCDIVTSFLL 286
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 25.8 bits (54), Expect = 8.3
Identities = 17/70 (24%), Positives = 30/70 (42%), Gaps = 1/70 (1%)
Frame = +3
Query: 447 GGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGFFSD-QSSARAKTFLSTA 623
GGR D + +K+G V E +E+ + + F S+ +S K + T
Sbjct: 186 GGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTY 245
Query: 624 QVVDDQVFAI 653
Q+ D+Q +
Sbjct: 246 QLPDNQTITL 255
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,259,908
Number of Sequences: 5004
Number of extensions: 61181
Number of successful extensions: 209
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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