Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_G22
(878 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 118 1e-27
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 45 2e-05
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 30 0.38
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.87
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.87
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.5
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 27 3.5
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 4.7
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 26 6.1
SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 26 6.1
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 26 6.1
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 6.1
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 6.1
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 6.1
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 26 8.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 8.1
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 26 8.1
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 26 8.1
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 118 bits (284), Expect = 1e-27
Identities = 70/176 (39%), Positives = 96/176 (54%), Gaps = 10/176 (5%)
Frame = +3
Query: 285 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 461
+ GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +
Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147
Query: 462 MHGETPYEIMFGPDICGPGTKKVHVIFSYKG-------KNHLIKKDIRCKDDVYTHLYTL 620
M Y IMFGPD CG + VH IF +K + HL + T+LYTL
Sbjct: 148 MSNSIDYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTL 206
Query: 621 IVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPEAKKPEDWDDKPTIQTP 782
IVKPD T+EV I+ + V G L D+ LPP +I DPE KP DW D+P I P
Sbjct: 207 IVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADWVDEPEIPDP 262
Score = 38.7 bits (86), Expect = 0.001
Identities = 21/62 (33%), Positives = 28/62 (45%)
Frame = +3
Query: 693 DWDFLPPKKIKDPEAKKPEDWDDKPTIQTPKTRSLRIGTSLNTFQIRCHQT*NWDDEMDG 872
DWD P+ I DP+A KPEDW + + P + + +WDDE DG
Sbjct: 269 DWDEDAPRMIPDPDAVKPEDWLEDEPLYIPDPEA--------------QKPEDWDDEEDG 314
Query: 873 XW 878
W
Sbjct: 315 DW 316
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 44.8 bits (101), Expect = 2e-05
Identities = 52/194 (26%), Positives = 82/194 (42%), Gaps = 8/194 (4%)
Frame = +3
Query: 165 FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 332
F E+F + +W S W K+ G + L S D+ GL T + + + L S
Sbjct: 33 FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89
Query: 333 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 506
P + + P+V+ F VK + CG Y+ + + K++ E P I FG
Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148
Query: 507 CGPGTKKVHVIFSYKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 680
CG I SY GK HL + T +YTL+++ + T + D V +G
Sbjct: 149 CGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ-NTTVVIRRDQSVVYTG 207
Query: 681 DLEADWDFLPPKKI 722
D+ ++ P K I
Sbjct: 208 DVGTNFFHSPTKWI 221
>SPBC1773.09c |mug184||meiotically upregulated gene
Mug184|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 30.3 bits (65), Expect = 0.38
Identities = 20/77 (25%), Positives = 36/77 (46%), Gaps = 1/77 (1%)
Frame = +3
Query: 543 SYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLE-ADWDFLPPKK 719
S + K H KK +D Y+H+Y + P + + + ++ ES E + D+LP
Sbjct: 137 STRKKEHFNKKPSFPRDTEYSHIYNMKYDPRSGIGIRVKRQEPESLKKENNNSDYLPKSA 196
Query: 720 IKDPEAKKPEDWDDKPT 770
+K + P+D P+
Sbjct: 197 MKQKKG-GPKDSSKHPS 212
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 29.1 bits (62), Expect = 0.87
Identities = 33/152 (21%), Positives = 67/152 (44%)
Frame = -2
Query: 505 MSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRER 326
++G ++ VS S S+ S + P S + + + ++ S + +
Sbjct: 117 LTGNGVLQTTVSSS-SVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSS 175
Query: 325 A*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFI 146
+ + +SS SSS S + + + + +S P S + S SS + SS ++S+
Sbjct: 176 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSR-P 234
Query: 145 EDNASKLTTTKYNSFHFRFTVPPSSNKRQATN 50
++S +TT ++F TV PSS+ ++
Sbjct: 235 SSSSSFITTMSSSTFISTVTVTPSSSSSSTSS 266
>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 391
Score = 29.1 bits (62), Expect = 0.87
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +3
Query: 672 ESGDLEADWDFLPPKKIKDPEAKKPEDWDDKP 767
E L+ LP + K P AKKPED++ P
Sbjct: 157 EKNTLDLKHCLLPSPEYKAPPAKKPEDYEASP 188
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 28.3 bits (60), Expect = 1.5
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = -2
Query: 250 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 119
N N F + + S +SGNFS T Q DN S +T
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/45 (24%), Positives = 19/45 (42%)
Frame = +3
Query: 633 DNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPEAKKPEDWDDKP 767
D E+ ++N ++ + WD PP + K+ WD P
Sbjct: 198 DKKRELELNNTEISQKPKRSRWDQAPPSVTQVSTTKRRSRWDKAP 242
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 26.6 bits (56), Expect = 4.7
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 6/71 (8%)
Frame = +3
Query: 414 GGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 581
G +LKV + E M ETP +I++ + G+ +H K + KK++
Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673
Query: 582 R--CKDDVYTH 608
CK+D + +
Sbjct: 674 LLFCKEDEFLY 684
>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1315
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/36 (36%), Positives = 16/36 (44%)
Frame = -3
Query: 213 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*QRPSTT 106
C P FP N+QE H+ I AS Q + T
Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQLENCT 826
>SPAC23G3.05c |||regulator of G-protein signaling
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 343
Score = 26.2 bits (55), Expect = 6.1
Identities = 17/69 (24%), Positives = 33/69 (47%)
Frame = +3
Query: 426 LKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYT 605
+K+F + +K + +P+E+M P + P K H + ++R +DV T
Sbjct: 154 VKMFTQIIIEKYFNPASPHEVMLPPQLVQP---------ILDCKEHQRQDELRLFEDVET 204
Query: 606 HLYTLIVKP 632
+L ++KP
Sbjct: 205 YLLNFLLKP 213
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1254
Score = 26.2 bits (55), Expect = 6.1
Identities = 19/70 (27%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Frame = +3
Query: 576 DIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDL-EADWDFLPPKKIKDP----EAK 740
D K + L T I + ++ + + + K+E+ +L E D F+ ++ + P E+
Sbjct: 148 DSPTKKKSFKSLDTSIFQAEDQFRHPVSS-KLENSELSEVDKPFIASRRSRKPVSYAESD 206
Query: 741 KPEDWDDKPT 770
+ ED+DD PT
Sbjct: 207 EDEDFDDAPT 216
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 6.1
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -2
Query: 319 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 224
N +E F+P + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +3
Query: 171 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 314
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 26.2 bits (55), Expect = 6.1
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +3
Query: 684 LEADWDFLPPKKIKDPEAKKPEDWDD 761
++ D F+PP +I PE K +WD+
Sbjct: 94 VDEDPAFIPPARILFPEEKLSMEWDN 119
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = -2
Query: 343 LNLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 182
++LR RA L+ E+ + LS+ LK +++ FP SFP +Y + ++SS
Sbjct: 121 VDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 35/143 (24%), Positives = 62/143 (43%)
Frame = -2
Query: 511 PQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLR 332
P S I S SP S SS S + S S + + + T+ + + + R
Sbjct: 336 PTSSSSTISSSSSSPSSSSFSSTTSSS---KSSSSFSSTVSSSSSTSSSTLTSSSSSSSR 392
Query: 331 ERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQ 152
+ + SS + SSS S + V+ F ++ S ++ SH SS + SS ++
Sbjct: 393 PASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHN----STSSRSSSHSSSHSLSSLSSKP 448
Query: 151 FIEDNASKLTTTKYNSFHFRFTV 83
+ ++S L T+ +++ R TV
Sbjct: 449 ILTASSSSLLTSSSHTYE-RSTV 470
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +3
Query: 168 EEKFPDDSWESNWVYS 215
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/65 (24%), Positives = 29/65 (44%)
Frame = +3
Query: 555 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPE 734
KNH ++ K+ V L + DN Y+ ++ + K + L+ D P +K +
Sbjct: 995 KNHALRIGNEFKEKVNERYSKLEIDIDNVYQRMLLHAKKKYAALQLDSQGKPNLDVKGLD 1054
Query: 735 AKKPE 749
K+ E
Sbjct: 1055 MKRRE 1059
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 693 DWDFLPPKKIKDPEAKKPEDW 755
DWD +P ++ K EA KP DW
Sbjct: 209 DWDDVPWRE-KPLEAMKPRDW 228
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,803,721
Number of Sequences: 5004
Number of extensions: 86044
Number of successful extensions: 306
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 300
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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