Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP24_F_G04
         (876 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual      59   7e-10
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||...    38   0.002
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S...    32   0.093
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    29   0.87 
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   3.5  
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa...    26   8.1  
SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S...    26   8.1  
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |...    26   8.1  

>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 272

 Score = 59.3 bits (137), Expect = 7e-10
 Identities = 36/116 (31%), Positives = 64/116 (55%)
 Frame = +1

Query: 433 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 612
           N+I F EL    +  C      + + AE A +GP+   +  LA KY + I+    E++EK
Sbjct: 39  NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94

Query: 613 HSDILWNTAVVISDTGNVIGKHRKNHIPRVGDXTXSNYYMEGNTGHPVFATRYGKI 780
            S+I++N+ + I++ GN+ G +RK H+      T   ++ +G +  P+F T +GK+
Sbjct: 95  QSNIIYNSCIYITENGNLGGVYRKVHL----FDTERKHFKKG-SDFPIFETSFGKL 145


>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 322

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 35/130 (26%), Positives = 60/130 (46%)
 Frame = +1

Query: 304 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 483
           R  ++G+VQ  +A   D+  N Q   +     K+++ A + G N+I   E++N P+   T
Sbjct: 42  RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91

Query: 484 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 663
                + E  E  E  P+   L  +A      +    +   E+    L+NTA+V   +G 
Sbjct: 92  GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147

Query: 664 VIGKHRKNHI 693
           +I  HRK H+
Sbjct: 148 LIAVHRKIHL 157


>SPBC336.05c |||S-adenosylmethionine-
           dependentmethyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 378

 Score = 32.3 bits (70), Expect = 0.093
 Identities = 21/64 (32%), Positives = 31/64 (48%)
 Frame = -2

Query: 545 KVVVGPSSADSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 366
           ++ V   S ++A SH  CF   QN+  + +       +   +P T   F+ LLK AFF  
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289

Query: 365 LTGR 354
           L GR
Sbjct: 290 LFGR 293


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1610

 Score = 29.1 bits (62), Expect = 0.87
 Identities = 12/37 (32%), Positives = 23/37 (62%)
 Frame = +1

Query: 517  SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 627
            SAE+  + +      +   +++VS++LE DEKH D++
Sbjct: 979  SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 17/52 (32%), Positives = 28/52 (53%)
 Frame = -1

Query: 396 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRESVCGDVEVSL 241
           +FI+ CF    + +  GH D +++ S   +S GS    +G  S   D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709


>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
           Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 502

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = +3

Query: 135 NNLTGRDLEEFNRIHFGRRNNLEI 206
           NN+  R +EE N I  G+R  LE+
Sbjct: 8   NNILKRHIEEDNNIDNGKRKKLEL 31


>SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase
           Mde10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 512

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 9/33 (27%), Positives = 15/33 (45%)
 Frame = -3

Query: 802 SEADVHPRSCRIWSQIQDGRCYLPCSSWXSXNR 704
           S    H  SC++  Q +DG C++    +    R
Sbjct: 434 SSLSCHSDSCKVSCQNEDGTCFISAKDYIDGTR 466


>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 499

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
 Frame = +3

Query: 105 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 215
           E H +E +I + N+T  DL  F  + FG+ N   ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,450,405
Number of Sequences: 5004
Number of extensions: 70667
Number of successful extensions: 223
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)