Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP24_F_F04
         (876 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   120   4e-28
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   116   4e-27
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate...    27   2.7  
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto...    26   8.1  

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  120 bits (288), Expect = 4e-28
 Identities = 58/96 (60%), Positives = 70/96 (72%)
 Frame = +1

Query: 322 LXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 501
           L ++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126

Query: 502 DAPMFVVGVNLDPYDPSFKVISKCFLHHKLSCPTCK 609
           DAPMFVVGVNL+ ++PS KVIS          P  K
Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAK 162



 Score = 98.3 bits (234), Expect = 1e-21
 Identities = 46/63 (73%), Positives = 53/63 (84%), Gaps = 1/63 (1%)
 Frame = +3

Query: 138 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 314
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 315 GFL 323
           G L
Sbjct: 65  GKL 67



 Score = 84.2 bits (199), Expect = 2e-17
 Identities = 41/48 (85%), Positives = 44/48 (91%)
 Frame = +3

Query: 729 AQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVRL 872
           A  NIIP+STGAAKAVGKVIPALNGKLTGMAFRVP  +VSVVDLTV+L
Sbjct: 202 ASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTVKL 249



 Score = 69.3 bits (162), Expect = 7e-13
 Identities = 34/55 (61%), Positives = 35/55 (63%)
 Frame = +2

Query: 569 NASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXXXXDGPSGKLWRDGRGCS 733
           NASCTTNCLAPLAKVI+D F I EGLM             DGPS K WR GRG S
Sbjct: 149 NASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGAS 203


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  116 bits (279), Expect = 4e-27
 Identities = 56/96 (58%), Positives = 69/96 (71%)
 Frame = +1

Query: 322 LXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 501
           L ++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126

Query: 502 DAPMFVVGVNLDPYDPSFKVISKCFLHHKLSCPTCK 609
           DAPM+VVGVN + ++PS KVIS          P  K
Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAK 162



 Score =  101 bits (241), Expect = 2e-22
 Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
 Frame = +3

Query: 138 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 314
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 315 GFL 323
           G L
Sbjct: 65  GKL 67



 Score = 84.2 bits (199), Expect = 2e-17
 Identities = 41/48 (85%), Positives = 44/48 (91%)
 Frame = +3

Query: 729 AQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVRL 872
           A  NIIP+STGAAKAVGKVIPALNGKLTGMAFRVP  +VSVVDLTV+L
Sbjct: 202 ASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTVKL 249



 Score = 69.3 bits (162), Expect = 7e-13
 Identities = 34/55 (61%), Positives = 35/55 (63%)
 Frame = +2

Query: 569 NASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXXXXDGPSGKLWRDGRGCS 733
           NASCTTNCLAPLAKVI+D F I EGLM             DGPS K WR GRG S
Sbjct: 149 NASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGAS 203


>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
           transferase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 453

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 15/45 (33%), Positives = 26/45 (57%)
 Frame = -3

Query: 196 SIEARSTKRPMRPNPLIPIFDMLSASYRKFVE*LTTTTYAQVQDG 62
           ++ A+ TK P+RP  + P   + SA + ++   +  T  AQVQ+G
Sbjct: 179 ALAAKLTK-PIRPLWVTPASRLTSAQFEEYFTVVLVTASAQVQNG 222


>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
           Sec74|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 928

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 17/75 (22%), Positives = 32/75 (42%)
 Frame = -2

Query: 536 SRLTPTTNMGASALGADIITFLAPPSK*ADALSVVVKTPVDSTTYSAPAFPHGMALGSLS 357
           S+L+PT     + L A  ++     S          ++ +DS+TY     P   ++ SLS
Sbjct: 174 SQLSPTHTSTIAELAASTLSIFPSGSYAGSTFGSPSRSSIDSSTY----LPRSKSVNSLS 229

Query: 356 EKTAILLPLTXKESI 312
              +   P + + S+
Sbjct: 230 SNFSARTPASNQSSV 244


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,468,797
Number of Sequences: 5004
Number of extensions: 72627
Number of successful extensions: 219
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)