Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_D03
(893 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 191 1e-49
SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 190 2e-49
SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces ... 29 0.67
SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 29 0.89
SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 29 1.2
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 27 4.8
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 8.3
>SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 191 bits (465), Expect = 1e-49
Identities = 88/165 (53%), Positives = 123/165 (74%)
Frame = +3
Query: 135 IVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDA 314
+VDPF+RK+WYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLADLQ D
Sbjct: 20 VVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQKDE-- 77
Query: 315 ERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRV 494
E +FRK +L E +QG++ L +F+G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RV
Sbjct: 78 EHAFRKVKLRVEDIQGKSCLTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRV 137
Query: 495 FCIGFTNKDSLSQRKTCYAQHTQVRAIRKKMCEIITRDVTNSELR 629
F IGFT + + +KT YAQ +Q+RAIR+KM ++I ++ +R
Sbjct: 138 FVIGFTRRRANQVKKTTYAQSSQIRAIRQKMFQVIQNQTSSCSMR 182
Score = 33.5 bits (73), Expect = 0.041
Identities = 18/45 (40%), Positives = 29/45 (64%)
Frame = +1
Query: 631 EVVNKLIPDSIAKDIEKACHGIYPLRXCLHPKGESVEXAPFRDLE 765
E+V KLIP+ I ++IE+A I+PL+ L K + ++ AP D +
Sbjct: 183 ELVQKLIPEVIGREIERATGSIFPLQNVLVRKVKILK-APKHDAQ 226
Score = 31.5 bits (68), Expect = 0.17
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = +2
Query: 710 SVCIRKVKVLXXPRFEISKLMELH 781
+V +RKVK+L P+ + KL+ELH
Sbjct: 209 NVLVRKVKILKAPKHDAQKLLELH 232
>SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 190 bits (464), Expect = 2e-49
Identities = 88/165 (53%), Positives = 120/165 (72%)
Frame = +3
Query: 135 IVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDA 314
+VDPF+RKDWYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLADLQ D
Sbjct: 20 VVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQKDE-- 77
Query: 315 ERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRV 494
E SFRK +L E +QG++ L +F+G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+
Sbjct: 78 EHSFRKVKLRVEDIQGKSCLTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRI 137
Query: 495 FCIGFTNKDSLSQRKTCYAQHTQVRAIRKKMCEIITRDVTNSELR 629
F IGFT++ +KT YAQ +Q+RAI +KM ++I ++
Sbjct: 138 FVIGFTSRRVNQVKKTTYAQSSQIRAIHQKMFQVIQNQANGCSMK 182
Score = 34.3 bits (75), Expect = 0.024
Identities = 15/26 (57%), Positives = 20/26 (76%)
Frame = +1
Query: 631 EVVNKLIPDSIAKDIEKACHGIYPLR 708
E+V KLIP+ I + IEKA + IYPL+
Sbjct: 183 ELVQKLIPEVIGRAIEKATNNIYPLQ 208
Score = 31.1 bits (67), Expect = 0.22
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = +2
Query: 710 SVCIRKVKVLXXPRFEISKLMELH 781
+V +RKVK+L P+ + KL+ELH
Sbjct: 209 NVFVRKVKILKAPKHDAQKLLELH 232
>SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 461
Score = 29.5 bits (63), Expect = 0.67
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +1
Query: 607 TSLTLNSGEVVNKLIPDSIAKDI-EKACHGIYPLRXCLHPKGESVEXAPFRDLEVD 771
++LT+N ++ L PD +A D+ E P R H G +V A R+ +D
Sbjct: 352 STLTINPTKIAESLTPDLLATDLAEYLVRKGLPFRQTHHISGSAVRMAEERNTTLD 407
>SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1019
Score = 29.1 bits (62), Expect = 0.89
Identities = 27/111 (24%), Positives = 47/111 (42%), Gaps = 2/111 (1%)
Frame = +3
Query: 213 TLVNRTQG-TKIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHG 389
T VN+ TK+A KG +V++ L+ DAE + + F I EY++ N
Sbjct: 82 TSVNKANMLTKLAET--KGAAADVNI--LKRTKDAEENKKLFEKIIEYIRATNKKVGVFP 137
Query: 390 MDLTTDKLRWMVKKWQTLIE-ANIDVKTTDGYVLRVFCIGFTNKDSLSQRK 539
D T K + +W ++ E + D + C+ ++ L+ K
Sbjct: 138 KDKTQGK---FINEWDSIFEPVKSEFNLVDASLGLAKCLAIKDEQELANIK 185
>SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 308
Score = 28.7 bits (61), Expect = 1.2
Identities = 22/70 (31%), Positives = 36/70 (51%), Gaps = 2/70 (2%)
Frame = +3
Query: 270 VFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMV--KKWQTL 443
VF+V + + D R+ + +A Q R V C H MDL + LR +V ++ L
Sbjct: 86 VFDVESREFERDLVTFRNCLE-ATVANSPQAR-VFCLIHKMDLVQEDLRDLVFEERKAIL 143
Query: 444 IEANIDVKTT 473
+E + D++TT
Sbjct: 144 LETSKDLETT 153
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -1
Query: 437 LPFFNHPPELIGCEVHAVEVAEHITS 360
L H PEL+ C +VA+H+TS
Sbjct: 181 LQSMGHSPELVQCITCLPDVADHLTS 206
>SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1309
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +2
Query: 497 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNQKENV 589
LHW+ L+ +Q + P H+GQ S + NV
Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNV 1194
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,438,011
Number of Sequences: 5004
Number of extensions: 69254
Number of successful extensions: 195
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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