BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_A24
(874 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 28 1.5
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 2.0
SPBC32H8.01c ||SPBP22H7.10c|conserved fungal protein|Schizosacch... 27 2.6
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 27 2.6
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 26 6.1
SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 26 6.1
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 8.1
SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase |S... 26 8.1
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 26 8.1
>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 836
Score = 28.3 bits (60), Expect = 1.5
Identities = 19/52 (36%), Positives = 26/52 (50%)
Frame = +2
Query: 398 VIKQQKNIDLQEFTYHRESKQYCAIPSQIVIEVNLPLLSSTKECTLXCERTI 553
VI Q + I L++ SK+Y IP+ +E PL +K TL E TI
Sbjct: 144 VISQNRRIVLEKLANPNSSKEYETIPT---VENRHPLNKLSKTKTLAYEETI 192
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 543 KEQSLYLISEKPAKYKLNISLPYPVNEECGNAKFDKSL 656
+E+S YL+S K A +N L + E GN F ++L
Sbjct: 464 EEESQYLLSTKDAINAINTLLNFQEQSEDGNVLFTRTL 501
>SPBC32H8.01c ||SPBP22H7.10c|conserved fungal
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 27.5 bits (58), Expect = 2.6
Identities = 20/62 (32%), Positives = 27/62 (43%), Gaps = 5/62 (8%)
Frame = +2
Query: 356 FNHITDNGYTIPKYVIKQQKN-IDLQEFTYHRESKQY----CAIPSQIVIEVNLPLLSST 520
+NH+ D G+ P VIK N ID + ESK P + E NL + +
Sbjct: 52 YNHLQDRGFATPISVIKSVTNKIDTNKLEAKDESKNVEKDATGNPQESKQEPNLEVQQTE 111
Query: 521 KE 526
KE
Sbjct: 112 KE 113
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.5 bits (58), Expect = 2.6
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = +1
Query: 55 RELVNNTAFXGLQKXYNIHLDVNNFRLPKSSYKGMAVPAVIR 180
R+++ TAF GL LD N F L S + A+ IR
Sbjct: 766 RQILGQTAFAGLMTVVLAMLDANTFVLDTSHWLLYAITLAIR 807
>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1826
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/40 (40%), Positives = 24/40 (60%)
Frame = -2
Query: 633 YHILHLLDREERCLIYIWLVFQISNIKIVLSHXKVHSLVE 514
Y + +LD RC+I I++VF IS + +V VH L+E
Sbjct: 1316 YMLKPVLDWIRRCIISIFIVFFISFLPLV-----VHDLLE 1350
>SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 578
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +2
Query: 329 KKPRKVLSEFNHITDNGYTI-PKYVIKQQKN 418
KKP + +EF H NGY + PK + +N
Sbjct: 530 KKPARYFAEFKHADLNGYLVKPKEYTMEGEN 560
>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 230
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = -1
Query: 451 LSVVCKFLKIYILLLFYDIFRYGISIISYVIK-F*KYFSGFFPLIYYRF 308
LS+ L + LLF+ F + S + ++ + F YFS FP++++ F
Sbjct: 110 LSLFRCLLLFFFFLLFFLSFSFSFSFLFFLSQIFIVYFSS-FPILHFLF 157
>SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 345
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 478 TWYSTVLFALSVVCKFLKIYILLLFYD 398
+WY TVL+A FL +Y++ L YD
Sbjct: 46 SWY-TVLWAFLPFWPFLIVYLIWLIYD 71
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 25.8 bits (54), Expect = 8.1
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = +2
Query: 260 PQHSYSDQNTEVFXXSKPVIDQWKKPRKVLSEFNHITDNGYTIPKYVIKQ 409
P H ++++ +++ S +I+ KPR S +H +NG + K V+K+
Sbjct: 878 PTH-FNEEESDIDSLSSSLIE---KPRASSSPIHHANNNGLRLLKDVLKK 923
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,355,922
Number of Sequences: 5004
Number of extensions: 69610
Number of successful extensions: 170
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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