Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP24_F_A13
(879 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 283 2e-77
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 275 5e-75
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 3e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 119 5e-28
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.1
SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 283 bits (694), Expect = 2e-77
Identities = 132/217 (60%), Positives = 161/217 (74%)
Frame = +1
Query: 223 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 402
CLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR
Sbjct: 25 CLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYR 84
Query: 403 QLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXX 582
LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 85 DLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGG 144
Query: 583 XXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTTQPLSTLTVLSWS 762
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTT L
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMV 204
Query: 763 TMKPSMTSAAXNLDIERPTYTNLNCLIGQIVSSITAS 873
+ NLDIERP+Y NLN LI Q+VSSITAS
Sbjct: 205 DNESCYDICRRNLDIERPSYENLNRLIAQVVSSITAS 241
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = +3
Query: 150 MRECISVHVGQAGVQIGNACWELY 221
MRE IS+HVGQAG QIGNACWELY
Sbjct: 1 MREIISIHVGQAGTQIGNACWELY 24
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 275 bits (675), Expect = 5e-75
Identities = 132/222 (59%), Positives = 162/222 (72%), Gaps = 5/222 (2%)
Frame = +1
Query: 223 CLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVR 387
CLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP V+D+VR
Sbjct: 25 CLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVR 83
Query: 388 TGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 567
TG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 84 TGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143
Query: 568 XXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTTQPLSTLT 747
LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTT L
Sbjct: 144 SFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSD 203
Query: 748 VLSWSTMKPSMTSAAXNLDIERPTYTNLNCLIGQIVSSITAS 873
+ NLDIERPTY NLN LI Q+VSSITAS
Sbjct: 204 CTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITAS 245
Score = 54.4 bits (125), Expect = 2e-08
Identities = 27/44 (61%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
Frame = +3
Query: 150 MRECISVHVGQAGVQIGNACWELY-LPGARHPA*WPDAHRQDHR 278
MRE ISVHVGQAGVQIGNACWELY L P +P + + H+
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHK 44
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 146 bits (355), Expect = 3e-36
Identities = 76/216 (35%), Positives = 116/216 (53%)
Frame = +1
Query: 229 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 408
EHG+ G T + N +F+E GK+VPRAV VDLEP +D V++G + L
Sbjct: 27 EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84
Query: 409 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 588
F P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 589 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTTQPLSTLTVLSWSTM 768
LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ Q +
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204
Query: 769 KPSMTSAAXNLDIERPTYTNLNCLIGQIVSSITASF 876
+ + A L I+ P+Y +LN L+ +++ +T SF
Sbjct: 205 EALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSF 240
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +3
Query: 150 MRECISVHVGQAGVQIGNACW 212
MRE + + GQ G Q+G A W
Sbjct: 1 MREIVHIQAGQCGNQVGAAFW 21
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 119 bits (287), Expect = 5e-28
Identities = 74/222 (33%), Positives = 119/222 (53%), Gaps = 5/222 (2%)
Frame = +1
Query: 223 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 402
CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV+ + + TY
Sbjct: 26 CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83
Query: 403 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 576
L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 577 XXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTTQPLSTLTVL 753
L+ERL+ Y KK ++++P Q VS VV+PYNS+L + TL
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKR--LTLNAD 200
Query: 754 SWSTMKPSMTS--AAXNLDIERPTYTNLNCLIGQIVSSITAS 873
S + + + AA L + PT+ N L+ ++S+ T +
Sbjct: 201 SVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTT 242
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +2
Query: 221 LAWSTASSLMARCPQTRPSGVETILSTLSSARPELAST 334
L ST SSL + ++PS T ST SSA P S+
Sbjct: 170 LTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 335
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +1
Query: 637 KKSKLEFAIYPAPQVSTAVVEPYNSILTTTQPLSTLTVLSW 759
KK KL+ A P P VST N + + PL L +LS+
Sbjct: 197 KKGKLDQAKIPPPLVSTL---NRNELFPSNGPLEVLEILSF 234
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 249 WPDAHRQDHRGWRRFFQHFLQR 314
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 25.8 bits (54), Expect = 8.1
Identities = 10/43 (23%), Positives = 22/43 (51%)
Frame = +1
Query: 616 RLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTTQPLSTL 744
+L D +L+F + +++ +V+P+ +T P S+L
Sbjct: 29 KLMSDRNANQELDFFFPKSKHIASTLVDPFGKTCSTASPASSL 71
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,541,267
Number of Sequences: 5004
Number of extensions: 74149
Number of successful extensions: 213
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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