Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_P07
(873 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 223 2e-59
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 135 9e-33
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 132 6e-32
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 89 1e-18
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 60 3e-10
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 2e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 50 4e-07
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 48 2e-06
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 40 6e-04
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 34 0.030
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.6
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 223 bits (546), Expect = 2e-59
Identities = 107/122 (87%), Positives = 115/122 (94%)
Frame = +1
Query: 469 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 648
KMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+R
Sbjct: 118 KMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMR 177
Query: 649 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMAXXXASTSLE 828
LDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ S+SLE
Sbjct: 178 LDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLE 237
Query: 829 KS 834
KS
Sbjct: 238 KS 239
Score = 159 bits (387), Expect = 4e-40
Identities = 69/74 (93%), Positives = 72/74 (97%)
Frame = +3
Query: 243 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 422
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 423 VLLTEAPLNPKANR 464
LLTEAPLNPK+NR
Sbjct: 103 CLLTEAPLNPKSNR 116
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +2
Query: 119 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 241
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 135 bits (326), Expect = 9e-33
Identities = 63/122 (51%), Positives = 86/122 (70%)
Frame = +1
Query: 469 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 648
KM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + R
Sbjct: 118 KMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGR 177
Query: 649 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMAXXXASTSLE 828
LD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E + +T L
Sbjct: 178 LDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLM 237
Query: 829 KS 834
++
Sbjct: 238 RN 239
Score = 81.0 bits (191), Expect = 2e-16
Identities = 32/67 (47%), Positives = 51/67 (76%)
Frame = +3
Query: 264 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 443
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 444 LNPKANR 464
+NP ANR
Sbjct: 110 MNPVANR 116
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2
Query: 131 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 229
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 132 bits (319), Expect = 6e-32
Identities = 58/110 (52%), Positives = 84/110 (76%)
Frame = +1
Query: 469 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 648
K+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI +
Sbjct: 123 KIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQ 182
Query: 649 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEM 798
++ GRD+TDYL L + G+ ++AE+EIVR+IKEK CYVA DF E+
Sbjct: 183 EEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232
Score = 82.6 bits (195), Expect = 7e-17
Identities = 38/69 (55%), Positives = 49/69 (71%), Gaps = 1/69 (1%)
Frame = +3
Query: 243 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEH 419
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 420 PVLLTEAPL 446
P+LLTE PL
Sbjct: 107 PLLLTEPPL 115
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +2
Query: 125 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 238
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 88.6 bits (210), Expect = 1e-18
Identities = 51/118 (43%), Positives = 73/118 (61%), Gaps = 10/118 (8%)
Frame = +1
Query: 478 QIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALP 633
+IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY +
Sbjct: 144 EIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIG 203
Query: 634 HAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALDFEQEMA 801
+I + LAGRD+T ++ +L +R S TAER IKE+ CYV D +E +
Sbjct: 204 SSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKEFS 256
Score = 77.0 bits (181), Expect = 3e-15
Identities = 40/98 (40%), Positives = 55/98 (56%), Gaps = 4/98 (4%)
Frame = +3
Query: 183 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 350
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 351 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 464
WD ME+ W + + LR PE+H LLTE PLNP NR
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENR 139
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +2
Query: 137 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 220
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 60.5 bits (140), Expect = 3e-10
Identities = 27/77 (35%), Positives = 50/77 (64%)
Frame = +1
Query: 568 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR 747
+V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVN 216
Query: 748 DIKEKLCYVALDFEQEM 798
+IKE +CYV+ +F+++M
Sbjct: 217 EIKESVCYVSQNFKEDM 233
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 58.0 bits (134), Expect = 2e-09
Identities = 31/122 (25%), Positives = 60/122 (49%), Gaps = 2/122 (1%)
Frame = +1
Query: 472 MTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAIL 645
MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH +P+ G +
Sbjct: 134 MTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAK 193
Query: 646 RLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMAXXXASTSL 825
R+ G + YL+K+ + SF + C+V+ D+ ++A L
Sbjct: 194 RISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDYTHDIAHALDRDIL 253
Query: 826 EK 831
E+
Sbjct: 254 ER 255
Score = 42.7 bits (96), Expect = 7e-05
Identities = 21/67 (31%), Positives = 39/67 (58%), Gaps = 1/67 (1%)
Frame = +3
Query: 255 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 431
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 432 TEAPLNP 452
TE NP
Sbjct: 121 TEPLANP 127
Score = 37.5 bits (83), Expect = 0.002
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +2
Query: 59 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 232
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 50.0 bits (114), Expect = 4e-07
Identities = 29/95 (30%), Positives = 46/95 (48%)
Frame = +1
Query: 475 TQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLD 654
TQ FE P +A + ++ LYA G G+V+D G + PI +G + A +L
Sbjct: 166 TQFFFEECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLP 225
Query: 655 LAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 759
L GR +T +L +L E + ++ + DI E
Sbjct: 226 LGGRYMTQHLQNLLRESLPTLKSSGQYVSKEDITE 260
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 47.6 bits (108), Expect = 2e-06
Identities = 24/81 (29%), Positives = 40/81 (49%)
Frame = +1
Query: 469 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 648
K + MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 116 KTLETMFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQV 175
Query: 649 LDLAGRDLTDYLMKILTERGY 711
+G + D L + L ++ +
Sbjct: 176 QHFSGNAINDILAQTLRDKNF 196
Score = 46.0 bits (104), Expect = 7e-06
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +3
Query: 309 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 464
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENR 114
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +2
Query: 122 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 295
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 39.5 bits (88), Expect = 6e-04
Identities = 21/71 (29%), Positives = 37/71 (52%), Gaps = 2/71 (2%)
Frame = +3
Query: 264 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 437
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 438 APLNPKANRXE 470
NP++ R +
Sbjct: 109 PSWNPQSVRQQ 119
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +2
Query: 122 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 223
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 33.9 bits (74), Expect = 0.030
Identities = 16/83 (19%), Positives = 41/83 (49%)
Frame = +1
Query: 469 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 648
K+ I+F + + +++ + + +G + V+D G + + EG +P++ ++
Sbjct: 261 KILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNSRIK 320
Query: 649 LDLAGRDLTDYLMKILTERGYSF 717
++ G D+T MK+L + +
Sbjct: 321 INYGGDDITLLFMKLLMRSHFPY 343
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.6
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 611 STKVTLCPTPSSVWTW 658
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -2
Query: 347 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 234
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,001,027
Number of Sequences: 5004
Number of extensions: 59600
Number of successful extensions: 223
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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