SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP23_F_N16
         (845 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch...    42   1e-04
SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomy...    33   0.051
SPBC1703.04 |mlh1||MutL family protein Mlh1 |Schizosaccharomyces...    24   4.4  

>SPBC543.08 |||phosphoinositide biosynthesis protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 250

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 25/65 (38%), Positives = 31/65 (47%)
 Frame = +1

Query: 547 SYFSRSDNFFNQYFVKIGWFWTLVITVPYVLLTSYTTCCGKRRMIATAHMVRLLIATVFW 726
           SYF  S N  N  FVK GWFWT        L+  Y     KR  I    + R ++AT+ W
Sbjct: 51  SYFGNSKNLINLIFVKRGWFWT-------SLVYFYHAWDQKRNKIDFKFISRYIVATL-W 102

Query: 727 WGWTT 741
           W + T
Sbjct: 103 WMFVT 107


>SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 735

 Score = 33.1 bits (72), Expect = 0.051
 Identities = 21/50 (42%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
 Frame = -2

Query: 343 FIRVFLKFVRLVMFSF-KDLRYFYTIRFLHFH*AISLLHTHASWVQTLQT 197
           FIR  LKF  +V  S  K +R+ Y   F      ISLLH H    QT +T
Sbjct: 277 FIRTHLKFKTIVFLSSCKQVRFVYET-FRRMRPGISLLHLHGKQKQTTRT 325


>SPBC1703.04 |mlh1||MutL family protein Mlh1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 684

 Score = 24.2 bits (50), Expect(2) = 4.4
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = +3

Query: 384 KTNTRSFINSRSTNSNDSAHL*EDTFL*HKFKNSTLF 494
           K+N   FIN+R   S +  H  E+T+  +  K ++ F
Sbjct: 266 KSNLILFINNRLVESVELRHALEETYAKYLHKGASYF 302



 Score = 20.6 bits (41), Expect(2) = 4.4
 Identities = 6/17 (35%), Positives = 12/17 (70%)
 Frame = +3

Query: 606 LDISYHRALRFINIVYD 656
           LD++ H + R ++ +YD
Sbjct: 314 LDVNVHPSKRIVHFLYD 330


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,267,476
Number of Sequences: 5004
Number of extensions: 64942
Number of successful extensions: 148
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -