BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_N16
(845 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 42 1e-04
SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomy... 33 0.051
SPBC1703.04 |mlh1||MutL family protein Mlh1 |Schizosaccharomyces... 24 4.4
>SPBC543.08 |||phosphoinositide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 250
Score = 41.5 bits (93), Expect = 1e-04
Identities = 25/65 (38%), Positives = 31/65 (47%)
Frame = +1
Query: 547 SYFSRSDNFFNQYFVKIGWFWTLVITVPYVLLTSYTTCCGKRRMIATAHMVRLLIATVFW 726
SYF S N N FVK GWFWT L+ Y KR I + R ++AT+ W
Sbjct: 51 SYFGNSKNLINLIFVKRGWFWT-------SLVYFYHAWDQKRNKIDFKFISRYIVATL-W 102
Query: 727 WGWTT 741
W + T
Sbjct: 103 WMFVT 107
>SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 735
Score = 33.1 bits (72), Expect = 0.051
Identities = 21/50 (42%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -2
Query: 343 FIRVFLKFVRLVMFSF-KDLRYFYTIRFLHFH*AISLLHTHASWVQTLQT 197
FIR LKF +V S K +R+ Y F ISLLH H QT +T
Sbjct: 277 FIRTHLKFKTIVFLSSCKQVRFVYET-FRRMRPGISLLHLHGKQKQTTRT 325
>SPBC1703.04 |mlh1||MutL family protein Mlh1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 684
Score = 24.2 bits (50), Expect(2) = 4.4
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +3
Query: 384 KTNTRSFINSRSTNSNDSAHL*EDTFL*HKFKNSTLF 494
K+N FIN+R S + H E+T+ + K ++ F
Sbjct: 266 KSNLILFINNRLVESVELRHALEETYAKYLHKGASYF 302
Score = 20.6 bits (41), Expect(2) = 4.4
Identities = 6/17 (35%), Positives = 12/17 (70%)
Frame = +3
Query: 606 LDISYHRALRFINIVYD 656
LD++ H + R ++ +YD
Sbjct: 314 LDVNVHPSKRIVHFLYD 330
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,267,476
Number of Sequences: 5004
Number of extensions: 64942
Number of successful extensions: 148
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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