BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_N15
(931 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 38 0.003
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.2
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 2.8
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 23 3.0
SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 5.0
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 23 5.7
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.6
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 6.6
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 8.7
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 37.5 bits (83), Expect = 0.003
Identities = 27/83 (32%), Positives = 28/83 (33%), Gaps = 2/83 (2%)
Frame = -1
Query: 895 GXXGGGXGG--GXXGGXPGGXXXXXXXXXXXXXXXXGFXEGXXGGGPXGXXXXXEGVXXX 722
G GGG GG G GG P G G GGGP G G
Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243
Query: 721 WAXWXGGGXKXVXGGXXXFGGRP 653
+ GGG GG FGG P
Sbjct: 244 PGGF-GGGLGGFGGGPGGFGGGP 265
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.7 bits (61), Expect = 1.2
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +3
Query: 867 PPPXPPPXXPPXGXKKXPPP 926
PPP PPP P PPP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +1
Query: 676 PPPXXTXPPPPPXXPXNXKPPP 741
PP PPPPP +PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPP 26
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +3
Query: 867 PPPXPPPXXPPXGXKKXPPP 926
PPP PP PP PPP
Sbjct: 11 PPPPPPGFEPPSQPPPPPPP 30
Score = 26.6 bits (56), Expect = 5.0
Identities = 11/23 (47%), Positives = 12/23 (52%), Gaps = 2/23 (8%)
Frame = +1
Query: 679 PPXXTXPPPPPX--XPXNXKPPP 741
PP PPPPP P + PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPP 27
Score = 26.6 bits (56), Expect = 5.0
Identities = 11/22 (50%), Positives = 12/22 (54%), Gaps = 2/22 (9%)
Frame = +3
Query: 867 PPPXPPPXXPPXGXK--KXPPP 926
PP PPP PP G + PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPP 26
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.2
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +1
Query: 676 PPPXXTXPPPPPXXPXNXKPP 738
PPP + PPPP P PP
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPP 1728
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +1
Query: 676 PPPXXTXPPPPPXXPXNXKPPP 741
PPP PPPP P PP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPP 1728
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +3
Query: 867 PPPXPPPXXPPXGXKKXPPP 926
PPP PPP G PPP
Sbjct: 762 PPPPPPPGVAGAGPPPPPPP 781
Score = 23.0 bits (47), Expect(2) = 3.5
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -2
Query: 507 PPPPPPXAP 481
PPPPPP P
Sbjct: 775 PPPPPPPPP 783
Score = 22.2 bits (45), Expect(2) = 3.5
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -2
Query: 519 GXXXPPPPPP 490
G PPPPPP
Sbjct: 759 GGPPPPPPPP 768
Score = 22.2 bits (45), Expect(2) = 5.9
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -2
Query: 519 GXXXPPPPPP 490
G PPPPPP
Sbjct: 774 GPPPPPPPPP 783
Score = 22.2 bits (45), Expect(2) = 5.9
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2
Query: 507 PPPPPPXA 484
PPPPPP A
Sbjct: 777 PPPPPPPA 784
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 23.0 bits (47), Expect(2) = 3.0
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -2
Query: 507 PPPPPPXAP 481
PPPPPP P
Sbjct: 306 PPPPPPPPP 314
Score = 22.6 bits (46), Expect(2) = 3.0
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = -2
Query: 534 GGXFXGXXXPPPPPP 490
G G PPPPPP
Sbjct: 298 GDSANGGLPPPPPPP 312
>SPBC660.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 26.6 bits (56), Expect = 5.0
Identities = 15/34 (44%), Positives = 15/34 (44%)
Frame = -3
Query: 614 PPXFPXFXXRGGGGGXAPXXAPXXXGGGGXFXGR 513
PP P F R GGG P P G GG GR
Sbjct: 101 PPRGPLFGPRIGGGHHGPLHGP-HGGFGGRGGGR 133
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 23.0 bits (47), Expect(2) = 5.7
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -2
Query: 507 PPPPPPXAP 481
PPPPPP P
Sbjct: 945 PPPPPPPPP 953
Score = 21.4 bits (43), Expect(2) = 5.7
Identities = 7/14 (50%), Positives = 7/14 (50%)
Frame = -2
Query: 531 GXFXGXXXPPPPPP 490
G PPPPPP
Sbjct: 939 GVMPAFPPPPPPPP 952
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.6
Identities = 14/29 (48%), Positives = 14/29 (48%), Gaps = 1/29 (3%)
Frame = -1
Query: 925 GGGXFFXPXGGXXGGGXGGGXXG-GXPGG 842
G G F GG GGG GG G G GG
Sbjct: 24 GRGGFGGGRGGARGGGRGGARGGRGGRGG 52
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/25 (44%), Positives = 14/25 (56%), Gaps = 2/25 (8%)
Frame = -3
Query: 425 PPPPXPPP--XSXKDIFXAXFLXGG 357
PPPP PPP KD++ A + G
Sbjct: 1095 PPPPPPPPAEVEKKDLYLALYDFAG 1119
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.8 bits (54), Expect = 8.7
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -3
Query: 425 PPPPXPPPXSXKD 387
PPPP PPP + +D
Sbjct: 189 PPPPPPPPPAVED 201
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.308 0.146 0.502
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,877,079
Number of Sequences: 5004
Number of extensions: 25082
Number of successful extensions: 154
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 43 (21.9 bits)
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