Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_M22
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 41 3e-04
SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor |Schizo... 41 3e-04
SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory... 35 0.018
SPBC609.02 |ptn1||phosphatidylinositol-3,4,5-trisphosphate3-phos... 30 0.51
SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 29 0.88
SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schi... 29 1.2
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 29 1.2
SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 28 2.0
SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 28 2.0
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 2.7
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 27 3.6
SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 6.2
SPAC11D3.09 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Manual 26 6.2
SPBP8B7.12c |fta3|sma3|Sim4 and Mal2 associated |Schizosaccharom... 26 6.2
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 8.2
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 40.7 bits (91), Expect = 3e-04
Identities = 21/52 (40%), Positives = 28/52 (53%)
Frame = +3
Query: 699 LNLLRSAANRDHAPALYNLGLCYEMGLGVSVNEKMAMELYXSAATLHHPXAL 854
+N AA R+H +L +G Y MGLG S ++A Y AA + HP AL
Sbjct: 478 MNYYSRAAARNHIHSLIKIGDFYRMGLGTSAKPELAFSYYSQAAAI-HPSAL 528
>SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 512
Score = 40.7 bits (91), Expect = 3e-04
Identities = 22/52 (42%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +3
Query: 732 HAPALYNLGLCYEMG-LGVSVNEKMAMELYXSAATLHHPXALYNLGIYYGQG 884
++ ALY L +CY G L +NEK A LY AA L+H A Y + I G
Sbjct: 223 YSEALYLLAVCYGTGALRTEINEKEAYRLYKMAADLNHVQAAYRVAICLQMG 274
>SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory
factor |Schizosaccharomyces pombe|chr 2|||Manual
Length = 633
Score = 34.7 bits (76), Expect = 0.018
Identities = 20/61 (32%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +3
Query: 690 EDGLNLLRSAANRDHAPALYNLGLCYEMG-LGVSVNEKMAMELYXSAATLHHPXALYNLG 866
E+ L +R + ++A A+Y YE G V + + A +LY S+A L + +LY LG
Sbjct: 263 ENALYKIREFSELENAKAMYFDAYVYETGAFDVESDIQRAWDLYSSSANLGYTRSLYRLG 322
Query: 867 I 869
+
Sbjct: 323 V 323
>SPBC609.02 |ptn1||phosphatidylinositol-3,4,
5-trisphosphate3-phosphatase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 348
Score = 29.9 bits (64), Expect = 0.51
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = -2
Query: 229 GVHRLFSSDELELLTTPTVLRFSNWATLLSKVSRIRRETYFHMITFK 89
G+H+L+ +DEL++ T NW L + ET +H+ FK
Sbjct: 45 GIHKLYRNDELDVFKYLTTQLKDNWILL----NLCAEETVYHLELFK 87
>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 965
Score = 29.1 bits (62), Expect = 0.88
Identities = 20/77 (25%), Positives = 29/77 (37%), Gaps = 3/77 (3%)
Frame = +3
Query: 663 LAAIENGHYEDGLNLLRSAANRDHAPALYNL---GLCYEMGLGVSVNEKMAMELYXSAAT 833
L I G+Y +N+LR + AP+ G Y MGL + N + Y
Sbjct: 390 LGVIHRGYYNQAMNILRPYLPEEDAPSSSTYSEGGAFYAMGL-IHANHGRGVTEYLREQL 448
Query: 834 LHHPXALYNLGIYYGQG 884
H + G+ G G
Sbjct: 449 KHTEDEIVQYGLLLGIG 465
>SPAC1B3.10c |||SEL1 repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 680
Score = 28.7 bits (61), Expect = 1.2
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = +3
Query: 657 LGLAAIENGHYEDGLNLLRSAANRDHAPALYNLGLCYEMGLGVS 788
LGL +E YE+ +L AA + + L CY G G S
Sbjct: 416 LGLIRLEEARYEEAYHLFLRAATQKSVISYKYLADCYYNGTGTS 459
>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 684
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -2
Query: 178 TVLRFSNWATLLSKVSRIRRETYFHMITFKLIFTCIL 68
T F+N L K S ++R++Y H+ ++L+ C L
Sbjct: 194 TAEEFANVLLKLMKASTVKRKSYSHLGDYELVTNCSL 230
>SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 456
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = +3
Query: 717 AANRDHAPALYNLGLCYEMGLG 782
AA HAPA Y LG YE G+G
Sbjct: 397 AAMLGHAPACYVLGKFYETGVG 418
>SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr
1|||Manual
Length = 113
Score = 27.9 bits (59), Expect = 2.0
Identities = 20/78 (25%), Positives = 33/78 (42%)
Frame = -3
Query: 402 CGICILYESADLCTSQLLIHCSM*WHQSKFCAQNSIVCCLSRHSYTSSSIYKMKTNEMEY 223
CG C + + C +L H + W ++KF ++C ++S T Y+ T +Y
Sbjct: 37 CGQCEKFYACFQCHDELNTHPFLPWRKAKFHIP-CVICGACKNSLTVEE-YR-STVHCKY 93
Query: 222 IDFFHQMNSNC*QHQQCY 169
+ H N C H Y
Sbjct: 94 CN--HPFNPKCKNHAGYY 109
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 27.5 bits (58), Expect = 2.7
Identities = 7/31 (22%), Positives = 23/31 (74%)
Frame = +3
Query: 210 EKSLCTPSRWFSSYKCLSSCRNDGTNSKRWN 302
+++LC ++++++C+SS ++D +++ +N
Sbjct: 1689 QEALCAKLYFYTAFECMSSLKSDSHDTEEYN 1719
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -3
Query: 321 SKFCAQNSIVCCLSRHSYTSSSIYKMKTNEMEY 223
S+F A S++C L RH SSI M Y
Sbjct: 8 SRFSANQSVLCLLQRHRVFFSSISVKPRKRMVY 40
>SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 932
Score = 26.2 bits (55), Expect = 6.2
Identities = 17/50 (34%), Positives = 21/50 (42%), Gaps = 2/50 (4%)
Frame = +3
Query: 741 ALYNLGLCYEMG--LGVSVNEKMAMELYXSAATLHHPXALYNLGIYYGQG 884
A+Y L YE GVS + ELY +A + A LG Y G
Sbjct: 753 AMYELAKIYEQPDRYGVSATPERKFELYKQSAVYGYAAAQCKLGECYEHG 802
>SPAC11D3.09 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 394
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/25 (44%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = +3
Query: 621 DLGQV-LNSIENQLGLAAIENGHYE 692
D G + L S +N + + IENGH+E
Sbjct: 130 DCGDIPLTSYDNAVAIKQIENGHFE 154
>SPBP8B7.12c |fta3|sma3|Sim4 and Mal2 associated
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 220
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/42 (35%), Positives = 25/42 (59%)
Frame = -2
Query: 319 QVLCSKFHRLLFVPSFLHELKHL*DENQRDGVHRLFSSDELE 194
Q L + + L F+ S L+E +HL +EN + F+S+E+E
Sbjct: 89 QRLYDESNSLGFISSPLNETEHLSEENLKIESSITFTSEEIE 130
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2
Query: 332 YMEQWINNWLVHKSADSYK 388
+ME W WL H+S DSY+
Sbjct: 361 WMEIWC--WLTHRSVDSYR 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,444,020
Number of Sequences: 5004
Number of extensions: 70389
Number of successful extensions: 171
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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