Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_J13
(902 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.014
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.30
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.68
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.6
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.8
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 2.8
SPAC6C3.02c |||CHCH domain protein|Schizosaccharomyces pombe|chr... 27 3.6
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 4.8
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 6.4
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 26 8.4
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 26 8.4
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.1 bits (77), Expect = 0.014
Identities = 21/56 (37%), Positives = 22/56 (39%), Gaps = 2/56 (3%)
Frame = -1
Query: 653 PXXGPXXXPXPTHPPXLXAXAPAXPXEXXFXPXXPPXSMXXP--PPPPX*XQTPPP 492
P P P P+ PP L APA P P PP P PP P PPP
Sbjct: 424 PPSAPPSLP-PSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Score = 32.3 bits (70), Expect = 0.097
Identities = 35/147 (23%), Positives = 37/147 (25%), Gaps = 10/147 (6%)
Frame = -3
Query: 828 PPPPPPXXEXAGX-PXXXXGXX-PXXGXXKXXXSXXNXPGKXXXPXGXPXXXXXPXIXPX 655
PPPPPP AG P G P + S P P P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 654 -------PXXGAXPXAXPHPPPPFXXPXPGXAXXKXXFXXXXPXXHXXXPPPPXMXPNPX 496
P A + P P P P P PP P P
Sbjct: 398 RSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457
Query: 495 PXPXX-PGXRXKXPRNXXPPQXPTPFP 418
P P P P PP P P P
Sbjct: 458 PLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 30.3 bits (65), Expect = 0.39
Identities = 31/140 (22%), Positives = 33/140 (23%)
Frame = -3
Query: 837 KXXPPPPPPXXEXAGXPXXXXGXXPXXGXXKXXXSXXNXPGKXXXPXGXPXXXXXPXIXP 658
K PPPPPP G P G N G P P
Sbjct: 309 KRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRS 368
Query: 657 XPXXGAXPXAXPHPPPPFXXPXPGXAXXKXXFXXXXPXXHXXXPPPPXMXPNPXPXPXXP 478
P G P P P A P + P P P P P
Sbjct: 369 APSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTP---PVPTPPSLPP 425
Query: 477 GXRXKXPRNXXPPQXPTPFP 418
P + PP P P
Sbjct: 426 SAPPSLPPS-APPSLPMGAP 444
Score = 29.5 bits (63), Expect = 0.68
Identities = 29/136 (21%), Positives = 33/136 (24%), Gaps = 10/136 (7%)
Frame = -3
Query: 855 PXXXXXKXXPPPPPPXXEXAGX--PXXXXGXXPXXGXXKXXXSXXNXPGKXXXPXGXPXX 682
P + PPPPPP + P + P G
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASR 411
Query: 681 XXXPXIXPXPXX--GAXPXAXPHPPP------PFXXPXPGXAXXKXXFXXXXPXXHXXXP 526
P + P A P P PP P P P A P P
Sbjct: 412 TSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPP 471
Query: 525 PPPXMXPNPXPXPXXP 478
P P P P P P
Sbjct: 472 AAPAPPPAPAPAPAAP 487
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/54 (33%), Positives = 21/54 (38%), Gaps = 1/54 (1%)
Frame = -1
Query: 653 PXXGPXXXPXPTHPPXLXAXAP-AXPXEXXFXPXXPPXSMXXPPPPPX*XQTPP 495
P P P P+ PP L AP + P P PP + PP P PP
Sbjct: 416 PVPTPPSLP-PSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
Score = 28.7 bits (61), Expect = 1.2
Identities = 22/64 (34%), Positives = 22/64 (34%), Gaps = 2/64 (3%)
Frame = -1
Query: 701 PXGPPXXXPXL*FXXXPXXGPXXXPX--PTHPPXLXAXAPAXPXEXXFXPXXPPXSMXXP 528
P PP P L P P P P PP L AP P P PP P
Sbjct: 421 PSLPPSAPPSL-----PPSAPPSLPMGAPAAPP-LPPSAPIAPPLPAGMPAAPPLPPAAP 474
Query: 527 PPPP 516
PPP
Sbjct: 475 APPP 478
Score = 27.5 bits (58), Expect = 2.8
Identities = 21/74 (28%), Positives = 22/74 (29%)
Frame = -1
Query: 740 PFPXETXXEXXXXPXGPPXXXPXL*FXXXPXXGPXXXPXPTHPPXLXAXAPAXPXEXXFX 561
P P P PP P L P P P P P AP P
Sbjct: 416 PVPTPPSLPPSAPPSLPPSAPPSL-----PMGAPAAPPLPPSAPI----APPLPAGMPAA 466
Query: 560 PXXPPXSMXXPPPP 519
P PP + PP P
Sbjct: 467 PPLPPAAPAPPPAP 480
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 30.7 bits (66), Expect = 0.30
Identities = 20/83 (24%), Positives = 21/83 (25%)
Frame = -3
Query: 672 PXIXPXPXXGAXPXAXPHPPPPFXXPXPGXAXXKXXFXXXXPXXHXXXPPPPXMXPNPXP 493
P I P A P PP P P P P PP P P P
Sbjct: 147 PSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPL 206
Query: 492 XPXXPGXRXKXPRNXXPPQXPTP 424
P + PP P
Sbjct: 207 SQAPVANTSSRPSSFAPPAGHAP 229
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.5 bits (63), Expect = 0.68
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = -2
Query: 625 TPPTPPXXXPXPRPGPXKXXFXPXXPPXP 539
TPP PP P P P P PP P
Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 27.1 bits (57), Expect = 3.6
Identities = 17/56 (30%), Positives = 19/56 (33%), Gaps = 3/56 (5%)
Frame = -1
Query: 653 PXXGPXXXPXPTHPPXLXAXA-PAXPXEXXFXPXXPPXSMXXP--PPPPX*XQTPP 495
P P P PP + A P P P PP P PPPP + P
Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 26.6 bits (56), Expect = 4.8
Identities = 15/46 (32%), Positives = 18/46 (39%), Gaps = 3/46 (6%)
Frame = -1
Query: 623 PTHP---PXLXAXAPAXPXEXXFXPXXPPXSMXXPPPPPX*XQTPP 495
P HP P + + A P P PP S+ PP P PP
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPP 1728
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/31 (38%), Positives = 13/31 (41%)
Frame = -3
Query: 528 PPPPXMXPNPXPXPXXPGXRXKXPRNXXPPQ 436
PPPP P P P P K +N P Q
Sbjct: 13 PPPPGFEPPSQPPPPPPPGYVKKRKNKTPAQ 43
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.8
Identities = 20/79 (25%), Positives = 22/79 (27%)
Frame = -1
Query: 653 PXXGPXXXPXPTHPPXLXAXAPAXPXEXXFXPXXPPXSMXXPPPPPX*XQTPPPXXXXQG 474
P P P T P A P P P P S PP P T PP
Sbjct: 991 PPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPV--PSTAPPVPIPTS 1048
Query: 473 NEXRXQETXXXPKXQPXFP 417
+ + P P P
Sbjct: 1049 TPPVPKSSSGAPSAPPPVP 1067
Score = 26.6 bits (56), Expect = 4.8
Identities = 17/54 (31%), Positives = 18/54 (33%)
Frame = -1
Query: 653 PXXGPXXXPXPTHPPXLXAXAPAXPXEXXFXPXXPPXSMXXPPPPPX*XQTPPP 492
P P P PP + AP P P PP S P P P P P
Sbjct: 1180 PVPKPAAGVPPVPPP---SEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVP 1230
Score = 25.8 bits (54), Expect = 8.4
Identities = 21/90 (23%), Positives = 23/90 (25%), Gaps = 3/90 (3%)
Frame = -3
Query: 660 PXPXXGAXPXAXPHPPPPFXXPXPG-XAXXKXXFXXXXPXXHXXXPPPPXMXPN--PXPX 490
P P G P P PP P G P PP P P P
Sbjct: 1182 PKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPA 1241
Query: 489 PXXPGXRXKXPRNXXPPQXPTPFPXXXAXS 400
P PR+ P P + S
Sbjct: 1242 PSSEAPSVSTPRSSVPSPHSNASPSPTSSS 1271
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.5 bits (58), Expect = 2.8
Identities = 13/35 (37%), Positives = 14/35 (40%)
Frame = -3
Query: 528 PPPPXMXPNPXPXPXXPGXRXKXPRNXXPPQXPTP 424
PPP + P P P PG P PP P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGP---PPPPPPPP 783
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/38 (31%), Positives = 14/38 (36%)
Frame = -1
Query: 605 LXAXAPAXPXEXXFXPXXPPXSMXXPPPPPX*XQTPPP 492
L +P P P P + PPP P PPP
Sbjct: 727 LLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP 764
Score = 26.6 bits (56), Expect = 4.8
Identities = 19/63 (30%), Positives = 21/63 (33%), Gaps = 2/63 (3%)
Frame = -1
Query: 653 PXXGPXXXPXPTHPPXLXAXAPAXPXEXXFXPXXPPXSMX--XPPPPPX*XQTPPPXXXX 480
P P PT P P P P PP + PPPPP PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP----PPPPAVSA 787
Query: 479 QGN 471
G+
Sbjct: 788 GGS 790
Score = 25.8 bits (54), Expect = 8.4
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -3
Query: 828 PPPPPPXXEXAGXP 787
PPPPPP AG P
Sbjct: 763 PPPPPPGVAGAGPP 776
Score = 23.4 bits (48), Expect(2) = 5.7
Identities = 11/38 (28%), Positives = 11/38 (28%)
Frame = -3
Query: 720 PGKXXXPXGXPXXXXXPXIXPXPXXGAXPXAXPHPPPP 607
P P P P P P P PPPP
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 21.0 bits (42), Expect(2) = 5.7
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -3
Query: 828 PPPPPP 811
PPPPPP
Sbjct: 732 PPPPPP 737
>SPAC6C3.02c |||CHCH domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 172
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = -1
Query: 551 PPXSMXXPPPPPX*XQTPPPXXXXQGN 471
PP +M PP P Q PPP G+
Sbjct: 27 PPRTMAPPPAPSRVQQAPPPTAVQGGS 53
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect = 4.8
Identities = 22/80 (27%), Positives = 24/80 (30%)
Frame = +3
Query: 180 GXXGXKXDXXXXXGWGGXKXKXXXXXXXXXXXXXXXGFXGXXXXXGXXIWXXGXFXFXXX 359
G G G+GG + GF G G G F
Sbjct: 196 GSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG---PGGFGGGLG 252
Query: 360 GXGGGXGGXGLG*RXGXGPG 419
G GGG GG G G GPG
Sbjct: 253 GFGGGPGGFGGGPGGHGGPG 272
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 26.2 bits (55), Expect = 6.4
Identities = 13/28 (46%), Positives = 13/28 (46%)
Frame = +3
Query: 516 RGGGGXXHGXGGXXGXKXXFXGPGRGXG 599
RGG G G GG G G GRG G
Sbjct: 450 RGGRGGFGGRGGFGGRGGFGGGRGRGRG 477
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 8.4
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = -1
Query: 530 PPPPPX*XQTPPPXXXXQGNEXRXQETXXXPKXQPXFP 417
PPPPP Q PP + Q+T P+ P P
Sbjct: 206 PPPPPP-QQNYPPAASSSAPPMQYQQTAYPPQQAPYPP 242
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = +3
Query: 366 GGGXGGXGLG*RXGXGPGEXG 428
GGG GG GLG G G G G
Sbjct: 225 GGGLGGAGLGGLGGAGLGGFG 245
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,440,470
Number of Sequences: 5004
Number of extensions: 20057
Number of successful extensions: 162
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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