Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP23_F_J02
         (872 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|c...    93   6e-20
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    28   2.0  
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy...    27   2.6  
SPBC27B12.01c |mmm1|SPBC30B4.09c|Mdm10/Mdm12/Mmm1 complex subuni...    27   3.5  
SPBC3E7.09 |||Sad1-UNC-like C-terminal|Schizosaccharomyces pombe...    27   3.5  
SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc...    26   8.1  

>SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 716

 Score = 92.7 bits (220), Expect = 6e-20
 Identities = 57/155 (36%), Positives = 83/155 (53%), Gaps = 8/155 (5%)
 Frame = +3

Query: 231 PENIIVNQPSDPLSRWF--TDCCV----SPQFIVIKLDSPSIVETIKFGKYIKALVSDLK 392
           P NI+++ P    SRW   T  C       Q++++KLD  ++V  I FGK+    + +LK
Sbjct: 22  PINILIDNPHQANSRWSCNTSICKPNNSEEQYVILKLDKLALVTDITFGKFHTPHLCNLK 81

Query: 393 KFQVYGGTEDSNMSLLLTAGLKKDSTPETFRLRHRTQEGFYFPV--EYLKIVPLQSWGPP 566
            F VYGG E   M  LL AGL  DS  ETF L  +        +  +Y+KIVPLQ+    
Sbjct: 82  DFIVYGGREQHLMFPLLRAGLTNDSNRETFPLCIKKYNKIENSIACKYIKIVPLQAHAHE 141

Query: 567 YNYTIWYIELHGKNQAELLNEALETIYLRKEEEAV 671
           +N +IWY+ LHG + A  L+   +   L  E++A+
Sbjct: 142 FNMSIWYVALHGIDSASALSCTEKNRALHLEKKAL 176


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 21/83 (25%), Positives = 39/83 (46%)
 Frame = +3

Query: 297 SPQFIVIKLDSPSIVETIKFGKYIKALVSDLKKFQVYGGTEDSNMSLLLTAGLKKDSTPE 476
           S  F  ++  S S+++     +  K +++ L+ +Q      D   +LL  + L K  +  
Sbjct: 274 SLHFFALQFFSTSLIQYTHICR--KCVITILQSYQQL--IVDQPANLLKFSLLSKKVSHF 329

Query: 477 TFRLRHRTQEGFYFPVEYLKIVP 545
            F L  +  E F+FP E+ K+ P
Sbjct: 330 LFTLAQKNIESFFFPTEWDKLKP 352


>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 589

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 27/104 (25%), Positives = 43/104 (41%), Gaps = 1/104 (0%)
 Frame = +3

Query: 321 LDSPSIVETIKFGKYIKALVSDLKKFQVYGGTEDSNMSLLLTAGLKKDSTPETFRLRHRT 500
           ++  S VE+I  GK  K  V    K  +     D     +L   +      E F ++H+ 
Sbjct: 256 IEDKSHVESIMTGKVTKVPVKADSKNTLSRRMTDRQALAMLKDSVTSHDPVEYFNVKHKL 315

Query: 501 QEGFYFPVEYLKIVPLQSWGPPYNYTIWYIELHGKNQAEL-LNE 629
            +G    V   K+V  +  G   +  I  I+L  + + EL LNE
Sbjct: 316 GQGASGSVYLAKVVGGKQLGIFDSVAIKSIDLQCQTRKELILNE 359


>SPBC27B12.01c |mmm1|SPBC30B4.09c|Mdm10/Mdm12/Mmm1 complex subunit
           Mmm1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 346

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 9/14 (64%), Positives = 12/14 (85%)
 Frame = +3

Query: 267 LSRWFTDCCVSPQF 308
           + +WFT+ CVSPQF
Sbjct: 302 IRQWFTNRCVSPQF 315


>SPBC3E7.09 |||Sad1-UNC-like C-terminal|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 659

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 32/128 (25%), Positives = 53/128 (41%), Gaps = 5/128 (3%)
 Frame = +3

Query: 288 CCVSPQFIVIKLDSPSIVETIKFGKYIKALVSDLKKFQV-----YGGTEDSNMSLLLTAG 452
           C    +F+VI+L     V+T++   + +   S  + F+V     Y   E S M L     
Sbjct: 230 CSAENKFVVIELCEDIYVDTVQIANF-EFFSSIFRDFKVSVSGKYPKYESSWMELGTFTA 288

Query: 453 LKKDSTPETFRLRHRTQEGFYFPVEYLKIVPLQSWGPPYNYTIWYIELHGKNQAELLNEA 632
           L    T ++F +     E      +YLKI  L  +G  +   +  + ++GK   E   EA
Sbjct: 289 LNL-RTLQSFHI-----ENPLIWAKYLKIEFLTHYGSEFYCPVSLLRVYGKTMIEEFEEA 342

Query: 633 LETIYLRK 656
            E    +K
Sbjct: 343 NEDFLEQK 350


>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
           Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 363

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -3

Query: 585 TIWYNCREGPTTVVGQSLGIQQGSKI 508
           TI  NC+ GP  V+G ++ I  G ++
Sbjct: 263 TIGKNCKIGPNVVIGPNVTIGDGVRL 288


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,885,765
Number of Sequences: 5004
Number of extensions: 62709
Number of successful extensions: 163
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)