Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_I21
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 124 2e-29
SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 107 2e-24
SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 105 8e-24
SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 94 2e-20
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 84 3e-17
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 33 0.072
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.38
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 28 1.5
SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr 1... 28 2.0
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 2.7
SPBC947.15c |||NADH dehydrogenase|Schizosaccharomyces pombe|chr ... 27 2.7
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 3.6
SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc... 27 3.6
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 27 4.7
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 4.7
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 26 6.2
>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 124 bits (299), Expect = 2e-29
Identities = 67/209 (32%), Positives = 112/209 (53%), Gaps = 2/209 (0%)
Frame = +3
Query: 267 ALVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACEL 446
A+ +RS T++T+ VL+A L V+G G + +D+D A ++G+ V N+P AN+ S EL
Sbjct: 100 AIGIRSKTRLTRRVLEAADSLIVIGCFCIGTNQVDLDFAAERGIAVFNSPYANSRSVAEL 159
Query: 447 TCTLMLVLARHVVPASTALKAGRWDRALYTGSELAGKTLAILGLGRVGREVATRMYAFGM 626
++ LAR V S L G W++ E+ GKTL I+G G +G +++ A G+
Sbjct: 160 VIGYIISLARQVGDRSLELHRGEWNKVSSGCWEIRGKTLGIIGYGHIGSQLSVLAEAMGL 219
Query: 627 NIIGFD--PFVSADQCAQFHCTKMELEDIWPLADYITLHTPLIESTRNFINADVLKQCKK 800
+++ +D P + Q L ++ AD+++LH P T+N I++ K+
Sbjct: 220 HVVYYDILPIMPLGSAKQL----SSLPELLHRADFVSLHVPASPETKNMISSKEFAAMKE 275
Query: 801 GVKIINVGRGGLIQXTXXLQALKSGKVGG 887
G +IN RG ++ + A KSGK+ G
Sbjct: 276 GSYLINASRGTVVDIPALVDASKSGKIAG 304
>SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 107 bits (258), Expect = 2e-24
Identities = 59/187 (31%), Positives = 100/187 (53%), Gaps = 1/187 (0%)
Frame = +3
Query: 330 KVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKA 509
K++ AG +N+D+ +A G+ V+ P + + E T L+L L R + A ++
Sbjct: 70 KLIALRCAGFNNVDLKAAADNGITVVRVPAYSPYAVAEYTIGLLLSLNRKIHRAYVRVRE 129
Query: 510 GRWDRALYTGSELAGKTLAILGLGRVGREVATRM-YAFGMNIIGFDPFVSADQCAQFHCT 686
++ G +L GKT+ +LG GR+G VA + FG ++ D + +F
Sbjct: 130 DDFNLNGLLGHDLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHD-IKPNKELEKFGIQ 188
Query: 687 KMELEDIWPLADYITLHTPLIESTRNFINADVLKQCKKGVKIINVGRGGLIQXTXXLQAL 866
+E +++ AD++ LH PL T + ++ +L KKGVKIIN RGGL+ ++A+
Sbjct: 189 FVEQQEVLAKADFLCLHCPLTPDTEHLVDEKLLASMKKGVKIINTSRGGLVDTKALVKAI 248
Query: 867 KSGKVGG 887
+SG+VGG
Sbjct: 249 ESGQVGG 255
>SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 105 bits (252), Expect = 8e-24
Identities = 62/198 (31%), Positives = 110/198 (55%), Gaps = 2/198 (1%)
Frame = +3
Query: 300 KEVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARH 479
K + + GVKL V R G G +N+++ +A + + V++ P + + E T L+L L R
Sbjct: 62 KALAENGVKL-VALRCG-GYNNVNLKAASEYKITVVHVPSYSPFAVSEFTVGLLLSLNRK 119
Query: 480 VVPASTALKAGRWDRALYTGSELAGKTLAILGLGRVGREVATRM-YAFGMNIIGFDPFVS 656
+ A ++ ++ G ++ GKT+ ++G G++G VA FG +++ +D ++
Sbjct: 120 IHRAYVRVREDDFNIVGLLGCDIHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYD--IN 177
Query: 657 ADQCAQFHCTK-MELEDIWPLADYITLHTPLIESTRNFINADVLKQCKKGVKIINVGRGG 833
D+ + + + +E ++ AD++ LH PL ST + +N+D L KKGV I+N RGG
Sbjct: 178 PDKKLENYGVQFVEQNEVLKKADFLCLHCPLTPSTTHIVNSDSLALMKKGVTIVNTSRGG 237
Query: 834 LIQXTXXLQALKSGKVGG 887
LI + A+ SG+VGG
Sbjct: 238 LIDTKALVDAIDSGQVGG 255
>SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 340
Score = 94.3 bits (224), Expect = 2e-20
Identities = 54/187 (28%), Positives = 95/187 (50%), Gaps = 1/187 (0%)
Frame = +3
Query: 324 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTAL 503
KL V G AG +N+DVD A + GV V N P + L + R A +L
Sbjct: 85 KLFVTG--AAGYNNVDVDWATRNGVYVANTPNGPTEGTANMNLMLFMCTLRGAREAEQSL 142
Query: 504 KAGRWDRALYTGSELAGKTLAILGLGRVGREVATRMYAFGMNIIGFD-PFVSADQCAQFH 680
+ G+W + L + GK + I+G+G +G+ A ++ G I+ + + A++ +
Sbjct: 143 RLGKWRQNLSLTDDPYGKRVGIIGMGAIGKSFAQKILPLGCEIVYHNRNRLEAEEEKRLG 202
Query: 681 CTKMELEDIWPLADYITLHTPLIESTRNFINADVLKQCKKGVKIINVGRGGLIQXTXXLQ 860
+ + +++ +D I+++ PL +T + I+ ++ K GV IIN RG +I ++
Sbjct: 203 ASFVSFDELLSSSDVISINCPLTPATHDLISTKEFEKMKDGVYIINTARGAIINEDAFIK 262
Query: 861 ALKSGKV 881
A+KSGKV
Sbjct: 263 AIKSGKV 269
>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 83.8 bits (198), Expect = 3e-17
Identities = 54/195 (27%), Positives = 94/195 (48%), Gaps = 1/195 (0%)
Frame = +3
Query: 300 KEVLD-AGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLAR 476
KE++D +K + GAG + +DV + +G+ V + P A + ++ LML R
Sbjct: 71 KEIIDNLPPSVKFICHLGAGYETVDVAACTARGIQVSHVPKAVDDATADVGIFLMLGALR 130
Query: 477 HVVPASTALKAGRWDRALYTGSELAGKTLAILGLGRVGREVATRMYAFGMNIIGFDPFVS 656
L W+ + GKTL ILGLG +G+ +A R AF M I+ +
Sbjct: 131 GFNQGIFELHKNNWNANCKPSHDPEGKTLGILGLGGIGKTMAKRARAFDMKIVYHNRTPL 190
Query: 657 ADQCAQFHCTKMELEDIWPLADYITLHTPLIESTRNFINADVLKQCKKGVKIINVGRGGL 836
++ A+ + +D+ +D ++L+ PL TR+ I ++ K+G+ I+N RG +
Sbjct: 191 PEEEAE-GAEFVSFDDLLAKSDVLSLNLPLNAHTRHIIGKPEFQKMKRGIVIVNTARGAV 249
Query: 837 IQXTXXLQALKSGKV 881
+ ++AL G V
Sbjct: 250 MDEAALVEALDEGIV 264
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 32.7 bits (71), Expect = 0.072
Identities = 26/84 (30%), Positives = 39/84 (46%), Gaps = 1/84 (1%)
Frame = -2
Query: 550 ASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVSS-HALSALAPGALITPTPFLPAES 374
+SSLP + S P+ S++ T+ + +V SS H + P L P+ F+ A+
Sbjct: 116 SSSLPSIPSSSSTPSISSIPHTTSSVSNDIPSVLGSSDHPIDLDNPEHLTPPSSFITAKQ 175
Query: 373 TSMLSTPAPARPTTFSFTPASNTS 302
S L TP P P + S P S
Sbjct: 176 LSRLPTPLP--PPSSSSLPTGTIS 197
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.3 bits (65), Expect = 0.38
Identities = 30/115 (26%), Positives = 48/115 (41%), Gaps = 2/115 (1%)
Frame = -2
Query: 601 ATSRPTLPRPRMARVLPASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVSSHALSAL 422
+TS PT ++ + + P + + +P + T + S +++
Sbjct: 232 STSIPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSI 291
Query: 421 AP-GALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 263
P G TP TP +P STS STP P P + S T S++ L + TT S
Sbjct: 292 PPTGNSTTPVTPTVPPTSTSSTSTPPP--PASTSSTGTSSSPLPSTSTSCTTSTS 344
Score = 29.9 bits (64), Expect = 0.51
Identities = 21/51 (41%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = -2
Query: 412 ALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 263
+L TP TP +P STS S P P PT+ S T +++ L T TT S
Sbjct: 186 SLSTPITPTVPPTSTSSTSIPIP--PTSTSSTDTNSSPLPTTSTSCTTSTS 234
Score = 29.9 bits (64), Expect = 0.51
Identities = 21/52 (40%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = -2
Query: 415 GALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 263
G TP TP +P STS STP P P + S T S++ L++ TT S
Sbjct: 349 GNSTTPVTPTVPPTSTSSTSTPPP--PASTSSTGTSSSPLLSTSTSCTTSTS 398
Score = 29.5 bits (63), Expect = 0.67
Identities = 21/51 (41%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -2
Query: 412 ALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 263
+L TP TP +P STS S P P PT+ S T ++ L T TT S
Sbjct: 129 SLSTPITPTVPPTSTSSTSIPIP--PTSTSSTDTNSNPLPTTSTSCTTSTS 177
Score = 26.2 bits (55), Expect = 6.2
Identities = 19/68 (27%), Positives = 27/68 (39%)
Frame = -2
Query: 466 TNINVQVSSHALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*V 287
T N S+ L P +T TP T+ S P + P T S S+++ VT
Sbjct: 528 TTTNCTTSTSVLYTSTP---VTSTPLATTNCTTSTSVPYTSTPVTSSNYTISSSTPVTST 584
Query: 286 AERTTRAS 263
TT +
Sbjct: 585 PVTTTNCT 592
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 28.3 bits (60), Expect = 1.5
Identities = 24/104 (23%), Positives = 52/104 (50%), Gaps = 3/104 (2%)
Frame = -2
Query: 610 ILVATSRPTLPRPRMARVLPASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVS---S 440
I+V++ T+ +P RV + + + P S + + + +++ Q S S
Sbjct: 427 IIVSSYVSTVTQPASTRVQTTTVSSISTSVKQ-PTASVASSSVSVPSSSSVQPQSSTPIS 485
Query: 439 HALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASN 308
+ SA +P + ++ + + +E +S L + + A P+T S TP+S+
Sbjct: 486 SSSSASSPQSTLSTSSEVVSEVSSTLLSGSSAIPSTSSSTPSSS 529
>SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 996
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -1
Query: 668 ALISRHERVESDDVHAEGVHPSGHLAADPAKAK 570
A+ HE VE V+ + VH G L DP K +
Sbjct: 112 AVFDNHEEVEVFPVYRDPVHDFGFLRFDPKKIR 144
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +3
Query: 51 VAARRSEEAHTSIKLNNDSTTKPRWSWTLSRF*SSTGLAPSVP 179
VAA R+ A ++ NND T P+ + +S F S G + + P
Sbjct: 416 VAALRARFAKANVSENNDPPTFPKTAAKISSFNSKAGTSFAKP 458
>SPBC947.15c |||NADH dehydrogenase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 551
Score = 27.5 bits (58), Expect = 2.7
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = -2
Query: 529 RARSHLPAFSAVEAGTTWRARTNI-NVQVSSHALSALAPGALITPTPFLPAESTSMLSTP 353
+ +S +P + V G+ W A I N+ S + ++ ++P TP LP+ + L P
Sbjct: 83 KGKSGVPKKNIVVLGSGWGAVAAIKNLDPSLYNITLVSPRDHFLFTPMLPSCTVGTLRLP 142
Query: 352 APARPTTFSF 323
+ P F
Sbjct: 143 SITEPIVALF 152
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 3.6
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
Frame = +3
Query: 546 LAGKTLAILGLGRVGREVATRMYAFGMNII--GFDPFVSADQCAQ--FHCTKME 695
+AGK + G G VG+ +T + + G +I DP ++A Q A F T ME
Sbjct: 212 IAGKVAVVAGFGDVGKGCSTSLRSQGARVIVTEVDP-INALQAAMDGFEVTTME 264
>SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 334
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 385 PAESTSMLSTPAPARPTTFSFTPASNTS 302
P+ ++ +PAP T S +PA+NTS
Sbjct: 125 PSSISNSSESPAPINSATASMSPANNTS 152
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/56 (26%), Positives = 26/56 (46%)
Frame = -2
Query: 436 ALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTR 269
++SAL+ + + PT + S S PAP P + S +P ++ V +R
Sbjct: 229 SVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTVKTTTSNASR 284
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = -2
Query: 535 VYRARSHLPAFSAVEAGTTWRARTNINVQVSSHALSALAPGA 410
+ R R HL + A E T WR + + + LS L P A
Sbjct: 334 IRRLRGHLREYVAWERNTIWREMMAMERRTQAARLSGLKPVA 375
>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 584
Score = 26.2 bits (55), Expect = 6.2
Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 4/70 (5%)
Frame = -1
Query: 587 DPAKAKDGEGLASELAASVQSPVPP-ASLQRSGSWDHVASQNQHQRAGQLARAQRI---G 420
DP+K+KDG L S V P PP + + +H + + R +L R Q G
Sbjct: 293 DPSKSKDGLNLGS--LTDVVPPQPPQPEHELAALQEHNEAHDAAMRQYELERQQNAMLPG 350
Query: 419 ARSVDHSDAL 390
S+D AL
Sbjct: 351 LASIDGLQAL 360
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,314,562
Number of Sequences: 5004
Number of extensions: 64497
Number of successful extensions: 260
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 230
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 254
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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