BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_H18
(929 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 33 0.076
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.10
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 30 0.40
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 30 0.53
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.8
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 6.6
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 6.6
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 32.7 bits (71), Expect = 0.076
Identities = 15/29 (51%), Positives = 15/29 (51%)
Frame = -1
Query: 710 PPPPPPXGXGXPXGPPPXXXFXXXPPPXG 624
PPPPPP G P PPP PPP G
Sbjct: 10 PPPPPPPGFEPPSQPPP-------PPPPG 31
Score = 26.6 bits (56), Expect = 5.0
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = -3
Query: 711 PTPPPPXXGWXPXGPPAP 658
P PPPP G+ P P P
Sbjct: 9 PPPPPPPPGFEPPSQPPP 26
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 32.3 bits (70), Expect = 0.10
Identities = 19/47 (40%), Positives = 19/47 (40%)
Frame = +2
Query: 572 GGGGGVXXPPXGGVXXXPXXGGXPGXXXXGAGGPXGXXPXXGGGGVG 712
GG GG P G GG PG G GG G P GGG G
Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGG-FGGGPGGFGGGPG 266
Score = 27.9 bits (59), Expect = 2.2
Identities = 20/54 (37%), Positives = 21/54 (38%), Gaps = 2/54 (3%)
Frame = +2
Query: 557 PXPXXGGGGGVXXPPXGGVXXXPXXG--GXPGXXXXGAGGPXGXXPXXGGGGVG 712
P P GG GG G G G PG G GG G P GGG+G
Sbjct: 200 PPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGG-FGGGPGGFGGGLG 252
Score = 27.5 bits (58), Expect = 2.8
Identities = 15/41 (36%), Positives = 15/41 (36%)
Frame = +2
Query: 557 PXPXXGGGGGVXXPPXGGVXXXPXXGGXPGXXXXGAGGPXG 679
P GG GG P G GG PG G GG G
Sbjct: 230 PGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270
Score = 27.1 bits (57), Expect = 3.8
Identities = 18/48 (37%), Positives = 18/48 (37%), Gaps = 1/48 (2%)
Frame = +2
Query: 575 GGGGVXXPPXGGVXXXPXX-GGXPGXXXXGAGGPXGXXPXXGGGGVGF 715
GG G GG P G PG G GG G GGG GF
Sbjct: 214 GGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGF 261
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.3 bits (65), Expect = 0.40
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = -1
Query: 707 PPPPPXGXGXPXGPPPXXXFXXXPPP 630
PPP P G P PPP PPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 29.5 bits (63), Expect = 0.71
Identities = 12/19 (63%), Positives = 12/19 (63%), Gaps = 2/19 (10%)
Frame = -1
Query: 710 PPPPPPX--GXGXPXGPPP 660
PPPPPP G G P PPP
Sbjct: 763 PPPPPPGVAGAGPPPPPPP 781
Score = 29.1 bits (62), Expect = 0.93
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = -1
Query: 710 PPPPPPXGXGXPXGPPPXXXFXXXPPP 630
PPP P G P PPP PPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 664 GGPXGXPXPXGGGGGGXXXXXPPP 735
GGP P P G G G PPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.5 bits (58), Expect = 2.8
Identities = 13/27 (48%), Positives = 13/27 (48%)
Frame = -3
Query: 711 PTPPPPXXGWXPXGPPAPXXFXPGXPP 631
P PPPP G GPP P P PP
Sbjct: 761 PPPPPPPPGVAGAGPPPP----PPPPP 783
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = -1
Query: 710 PPPPPPXGXGXPXGPPPXXXFXXXPPP 630
P PPP G P PPP PP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPP 776
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 29.9 bits (64), Expect = 0.53
Identities = 17/47 (36%), Positives = 18/47 (38%)
Frame = +2
Query: 572 GGGGGVXXPPXGGVXXXPXXGGXPGXXXXGAGGPXGXXPXXGGGGVG 712
GGG G GG+ G G G G P G GGGG G
Sbjct: 136 GGGMGGGMGGMGGMDDDMDMDGGFGTRTRGGGMPGGFANMFGGGGAG 182
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 3.8
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -2
Query: 706 PPPPLXGXGPXXAPRPXXXXPXXPPP 629
PPPP+ P AP P PPP
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPP 1733
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = -1
Query: 710 PPPPPPXGXGXPXGPPPXXXFXXXPPP 630
PPPPPP P P PPP
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPP 364
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/26 (38%), Positives = 11/26 (42%)
Frame = -2
Query: 706 PPPPLXGXGPXXAPRPXXXXPXXPPP 629
PP P G P+P P PPP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPP 1194
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,176,286
Number of Sequences: 5004
Number of extensions: 14679
Number of successful extensions: 206
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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