Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_G24
(901 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 288 6e-79
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 288 6e-79
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 288 6e-79
SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Sc... 48 2e-06
SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 30 0.39
SPAC1F12.10c |||NADPH-hemoprotein reductase |Schizosaccharomyces... 28 1.6
SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH comple... 26 6.3
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 8.4
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 288 bits (707), Expect = 6e-79
Identities = 131/186 (70%), Positives = 154/186 (82%)
Frame = +3
Query: 147 RRGKALLNFAL*XYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPE 326
R+G A L +AERHGYI+GVV+ IIHDPGRGAPLA V FR+PY ++T E F+A E
Sbjct: 22 RKGAAQLRTL--DFAERHGYIRGVVQKIIHDPGRGAPLAKVAFRNPYHYRTDVETFVATE 79
Query: 327 GLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHN 506
G+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+ ++GH+
Sbjct: 80 GMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNYVIIVGHD 139
Query: 507 PDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVR 686
D +TRVKLPSGAKKV+PSS RG+VGIVAGGGRIDKP+LKAGRA+HKY+VKRNCWP R
Sbjct: 140 VDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTR 199
Query: 687 GVAMNP 704
GVAMNP
Sbjct: 200 GVAMNP 205
Score = 44.0 bits (99), Expect = 3e-05
Identities = 21/32 (65%), Positives = 27/32 (84%)
Frame = +2
Query: 86 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSL 181
MGRVIRAQRK +G +F +HT+ RKGA +LR+L
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTL 31
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +2
Query: 722 GGGNHQHIGKAST 760
GGGNHQH+G ++T
Sbjct: 211 GGGNHQHVGHSTT 223
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 288 bits (707), Expect = 6e-79
Identities = 131/186 (70%), Positives = 154/186 (82%)
Frame = +3
Query: 147 RRGKALLNFAL*XYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPE 326
R+G A L +AERHGYI+GVV+ IIHDPGRGAPLA V FR+PY ++T E F+A E
Sbjct: 22 RKGAAQLRTL--DFAERHGYIRGVVQKIIHDPGRGAPLAKVAFRNPYHYRTDVETFVATE 79
Query: 327 GLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHN 506
G+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+ ++GH+
Sbjct: 80 GMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNYVIIVGHD 139
Query: 507 PDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVR 686
D +TRVKLPSGAKKV+PSS RG+VGIVAGGGRIDKP+LKAGRA+HKY+VKRNCWP R
Sbjct: 140 VDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTR 199
Query: 687 GVAMNP 704
GVAMNP
Sbjct: 200 GVAMNP 205
Score = 44.0 bits (99), Expect = 3e-05
Identities = 21/32 (65%), Positives = 27/32 (84%)
Frame = +2
Query: 86 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSL 181
MGRVIRAQRK +G +F +HT+ RKGA +LR+L
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTL 31
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +2
Query: 722 GGGNHQHIGKAST 760
GGGNHQH+G ++T
Sbjct: 211 GGGNHQHVGHSTT 223
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 288 bits (707), Expect = 6e-79
Identities = 131/186 (70%), Positives = 154/186 (82%)
Frame = +3
Query: 147 RRGKALLNFAL*XYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPE 326
R+G A L +AERHGYI+GVV+ IIHDPGRGAPLA V FR+PY ++T E F+A E
Sbjct: 22 RKGAAQLRTL--DFAERHGYIRGVVQKIIHDPGRGAPLAKVAFRNPYHYRTDVETFVATE 79
Query: 327 GLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHN 506
G+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+ ++GH+
Sbjct: 80 GMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNYVIIVGHD 139
Query: 507 PDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVR 686
D +TRVKLPSGAKKV+PSS RG+VGIVAGGGRIDKP+LKAGRA+HKY+VKRNCWP R
Sbjct: 140 VDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTR 199
Query: 687 GVAMNP 704
GVAMNP
Sbjct: 200 GVAMNP 205
Score = 44.0 bits (99), Expect = 3e-05
Identities = 21/32 (65%), Positives = 27/32 (84%)
Frame = +2
Query: 86 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSL 181
MGRVIRAQRK +G +F +HT+ RKGA +LR+L
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTL 31
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +2
Query: 722 GGGNHQHIGKAST 760
GGGNHQH+G ++T
Sbjct: 211 GGGNHQHVGHSTT 223
>SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit
L2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 318
Score = 47.6 bits (108), Expect = 2e-06
Identities = 33/111 (29%), Positives = 59/111 (53%)
Frame = +3
Query: 372 LEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAK 551
L+ GN P+ +P GT++ + + +L R++G+ A +I D K V+L SG +
Sbjct: 162 LKPGNCFPLRLIPIGTVIHAIGVNPNQKAKLCRSAGSSARIIAF--DGKYAIVRLQSGEE 219
Query: 552 KVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNP 704
+ + ++ +G+V+ + + KAGR+ ++ R P VRG AMNP
Sbjct: 220 RKILDTSFATIGVVSNIYWQHRQLGKAGRS--RWLGIR---PTVRGTAMNP 265
>SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 897
Score = 30.3 bits (65), Expect = 0.39
Identities = 17/57 (29%), Positives = 24/57 (42%)
Frame = -1
Query: 598 PATIPTMPLLLDGRTFLAPDGSFTLVRLASGLCPITVAKFPEARARRPLSPIFSSRL 428
PAT+P F P F +L SGL V+ +P+ + +P SRL
Sbjct: 246 PATVPITQAASTANAFQQPSNQFQTQKLPSGLDTRPVSSYPDELTQLESNPDSFSRL 302
>SPAC1F12.10c |||NADPH-hemoprotein reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 147
Score = 28.3 bits (60), Expect = 1.6
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +3
Query: 609 IDKPILKAGRAYHKYKVKRNCWPYVRGVAMN 701
++KPI K+K K +CW +RG N
Sbjct: 68 VEKPIPVTKEELAKHKTKEDCWIAIRGKVYN 98
>SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH complex
subunit Tfb2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 447
Score = 26.2 bits (55), Expect = 6.3
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +2
Query: 716 LTGGGNHQHIGKASTGQERNI 778
LTGGGNH G T +++++
Sbjct: 105 LTGGGNHNSFGVPCTDEDKHL 125
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 457 PLSPIFSSRLHTMVPSGIAPTGITFPTSRVAFF 359
P++P+F SR H P G+ T FP F+
Sbjct: 11 PINPVFPSRAHPSPPLGL--TSNLFPIQTNKFY 41
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,723,577
Number of Sequences: 5004
Number of extensions: 79784
Number of successful extensions: 209
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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