Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_G08
(832 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 440 e-125
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 427 e-122
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 321 4e-90
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 288 4e-80
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.5
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.5
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.5
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.6
AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 22 8.0
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 440 bits (1083), Expect = e-125
Identities = 207/212 (97%), Positives = 209/212 (98%)
Frame = +1
Query: 115 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 294
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 295 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 474
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 475 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 654
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 655 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFQ 750
IGYNPAAVAFVPISGWHGDNMLE S+KMPWF+
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFK 212
Score = 43.2 bits (97), Expect = 3e-06
Identities = 17/21 (80%), Positives = 19/21 (90%)
Frame = +2
Query: 746 FKGWQVERKEGKXDGKCLIES 808
FKGW VERKEGK +GKCLIE+
Sbjct: 211 FKGWTVERKEGKVEGKCLIEA 231
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 427 bits (1053), Expect = e-122
Identities = 200/212 (94%), Positives = 205/212 (96%)
Frame = +1
Query: 115 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 294
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 295 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 474
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 475 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 654
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 655 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFQ 750
IGYN A+VAFVPISGWHGDNMLEPS K PW++
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYK 212
Score = 39.5 bits (88), Expect = 4e-05
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +2
Query: 731 PKCLGFKGWQVERKEGKXDGKCLIES 808
PK +KGW+VERK+G DGK LIE+
Sbjct: 206 PKTPWYKGWKVERKDGNADGKTLIEA 231
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 321 bits (789), Expect = 4e-90
Identities = 151/155 (97%), Positives = 153/155 (98%)
Frame = +1
Query: 286 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 465
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 466 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 645
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 646 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFQ 750
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWF+
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFK 155
Score = 43.2 bits (97), Expect = 3e-06
Identities = 17/21 (80%), Positives = 19/21 (90%)
Frame = +2
Query: 746 FKGWQVERKEGKXDGKCLIES 808
FKGW VERKEGK +GKCLIE+
Sbjct: 154 FKGWTVERKEGKVEGKCLIEA 174
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 288 bits (707), Expect = 4e-80
Identities = 135/139 (97%), Positives = 137/139 (98%)
Frame = +1
Query: 334 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 513
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 514 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 693
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 694 SGWHGDNMLEPSTKMPWFQ 750
SGWHGDNMLE S+KMPWF+
Sbjct: 121 SGWHGDNMLEVSSKMPWFK 139
Score = 43.2 bits (97), Expect = 3e-06
Identities = 17/21 (80%), Positives = 19/21 (90%)
Frame = +2
Query: 746 FKGWQVERKEGKXDGKCLIES 808
FKGW VERKEGK +GKCLIE+
Sbjct: 138 FKGWTVERKEGKVEGKCLIEA 158
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 2e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +1
Query: 373 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 552
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 553 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 723
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.65
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 127 KTHINIVVIGHVDSGKST 180
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.5
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 136 INIVVIGHVDSGKST 180
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.5
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 128 FSLPIFG*SRITNCV*Y 78
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 358 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 459
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 358 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 459
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase
beta-3 protein.
Length = 832
Score = 21.8 bits (44), Expect = 8.0
Identities = 7/39 (17%), Positives = 16/39 (41%)
Frame = +3
Query: 693 FWMARRQHVGAFNQNALVSRDGRWSVRKAXLTENASLKA 809
+W+ +R+H + + + +W R +A A
Sbjct: 593 YWLEKREHRSSSTKGITIQEPPQWHTRSTEKRVSAGTPA 631
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 236,628
Number of Sequences: 438
Number of extensions: 5087
Number of successful extensions: 24
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 26581563
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -