Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_C02
(873 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 444 e-127
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 433 e-123
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 325 3e-91
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 292 2e-81
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.9
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 444 bits (1093), Expect = e-127
Identities = 217/236 (91%), Positives = 218/236 (92%), Gaps = 1/236 (0%)
Frame = +1
Query: 109 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 288
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 289 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 468
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 469 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 648
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 649 IGYNPAAVAFVPISGWHGDNMLEPSXKC-LGSXMAGGA*ESKADGKCLIEALDAIL 813
IGYNPAAVAFVPISGWHGDNMLE S K E K +GKCLIEALDAIL
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAIL 236
Score = 49.6 bits (113), Expect = 4e-08
Identities = 23/43 (53%), Positives = 27/43 (62%)
Frame = +2
Query: 743 GWQVERKKAKLTENASLKLSMPSWPPXRPTDKPLRLPLQXVYK 871
GW VERK+ K+ ++ PP RPTDK LRLPLQ VYK
Sbjct: 213 GWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYK 255
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 433 bits (1066), Expect = e-123
Identities = 214/238 (89%), Positives = 217/238 (91%), Gaps = 3/238 (1%)
Frame = +1
Query: 109 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 288
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 289 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 468
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 469 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 648
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 649 IGYNPAAVAFVPISGWHGDNMLEPSXKCLGSXMAGGA*ESK---ADGKCLIEALDAIL 813
IGYN A+VAFVPISGWHGDNMLEPS K G E K ADGK LIEALDAIL
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKT--PWYKGWKVERKDGNADGKTLIEALDAIL 236
Score = 46.8 bits (106), Expect = 3e-07
Identities = 22/43 (51%), Positives = 25/43 (58%)
Frame = +2
Query: 743 GWQVERKKAKLTENASLKLSMPSWPPXRPTDKPLRLPLQXVYK 871
GW+VERK ++ PP RPTDK LRLPLQ VYK
Sbjct: 213 GWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYK 255
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 325 bits (799), Expect = 3e-91
Identities = 161/179 (89%), Positives = 162/179 (90%), Gaps = 1/179 (0%)
Frame = +1
Query: 280 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 459
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 460 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 639
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 640 IKKIGYNPAAVAFVPISGWHGDNMLEPSXKC-LGSXMAGGA*ESKADGKCLIEALDAIL 813
IKKIGYNPAAVAFVPISGWHGDNMLE S K E K +GKCLIEALDAIL
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAIL 179
Score = 49.6 bits (113), Expect = 4e-08
Identities = 23/43 (53%), Positives = 27/43 (62%)
Frame = +2
Query: 743 GWQVERKKAKLTENASLKLSMPSWPPXRPTDKPLRLPLQXVYK 871
GW VERK+ K+ ++ PP RPTDK LRLPLQ VYK
Sbjct: 156 GWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYK 198
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 292 bits (717), Expect = 2e-81
Identities = 145/163 (88%), Positives = 146/163 (89%), Gaps = 1/163 (0%)
Frame = +1
Query: 328 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 507
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 508 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 687
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 688 SGWHGDNMLEPSXKC-LGSXMAGGA*ESKADGKCLIEALDAIL 813
SGWHGDNMLE S K E K +GKCLIEALDAIL
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAIL 163
Score = 32.3 bits (70), Expect = 0.006
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +2
Query: 743 GWQVERKKAKLTENASLKLSMPSWPPXRPTDK 838
GW VERK+ K+ ++ PP RPTDK
Sbjct: 140 GWTVERKEGKVEGKCLIEALDAILPPTRPTDK 171
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 2e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +1
Query: 367 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 546
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 547 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 717
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.69
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 121 KTHINIVVIGHVDSGKST 174
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 130 INIVVIGHVDSGKST 174
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.7
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -2
Query: 122 FSLPIFG*SRITNCV*Y 72
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 352 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 453
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 352 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 453
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 239,663
Number of Sequences: 438
Number of extensions: 4856
Number of successful extensions: 25
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 28280841
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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