Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP23_F_B05
(890 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 56 5e-09
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 56 9e-09
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 45 2e-05
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 32 0.13
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 28 2.1
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 27 2.7
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 27 3.6
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 56.4 bits (130), Expect = 5e-09
Identities = 37/153 (24%), Positives = 63/153 (41%), Gaps = 1/153 (0%)
Frame = +2
Query: 278 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKCYESVFANGCEECNKIIGI 457
G A K H F C C ++L + R+ YC Y F+ C+ C I
Sbjct: 268 GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCK--TPI 325
Query: 458 DSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVF 634
+ + + + +HE CA C V V+ + D +C CYD ++A +C C +
Sbjct: 326 EDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPI 385
Query: 635 RAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSF 733
+ K ++H +C+ C C +G + +
Sbjct: 386 LGISVK--GSDGEYHSQCWTCGACNALLGDEGY 416
Score = 37.9 bits (84), Expect = 0.002
Identities = 21/73 (28%), Positives = 27/73 (36%)
Frame = +2
Query: 404 YESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGN 583
Y C C + + +S K H CF C C +L F + K YC
Sbjct: 250 YRGNSEKSCHSCGGSLRA-GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308
Query: 584 CYDAQFASRCDGC 622
Y QF+ RC C
Sbjct: 309 DYHEQFSPRCKHC 321
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/42 (26%), Positives = 19/42 (45%)
Frame = +2
Query: 278 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKC 403
G K + ++HS + C C+ L + Y + + P C C
Sbjct: 387 GISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 55.6 bits (128), Expect = 9e-09
Identities = 33/104 (31%), Positives = 44/104 (42%), Gaps = 3/104 (2%)
Frame = +2
Query: 308 WHSGHFCCWQCDESLTGQRYVL---RDEHPYCIKCYESVFANGCEECNKIIGIDSKDLSY 478
WH FCC +CD+ L +L D P C C A+ C C I D +S
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIK-DYALMSG 93
Query: 479 KDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASR 610
D + H CF C CR ++D F I+C +C + SR
Sbjct: 94 YDSY-HRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
Score = 37.1 bits (82), Expect = 0.003
Identities = 26/105 (24%), Positives = 39/105 (37%), Gaps = 3/105 (2%)
Frame = +2
Query: 428 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKLD-KIYCGNCYDAQ 598
C C + +S+ WH+ CF C KC L D+ D + C +C
Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC---- 74
Query: 599 FASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSF 733
A C C + Y + +H +CF C C+ I +F
Sbjct: 75 -AHTCTACRMRIKDYALMSGYDS--YHRECFRCHDCRKQIIDSNF 116
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 44.8 bits (101), Expect = 2e-05
Identities = 35/145 (24%), Positives = 58/145 (40%), Gaps = 8/145 (5%)
Frame = +2
Query: 278 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRD-----EHPYCIKCYESVFANGCEECN 442
G+Y +A+ +H F C C+ + + + + D + P C Y C C
Sbjct: 125 GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCG 184
Query: 443 KIIGIDSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDG 619
+ + ++ +K +H F C+ C V + + K+YC Y FA+RC G
Sbjct: 185 --MALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCG 242
Query: 620 C-GEVFRAGTKKMEYKTRQ-WHEKC 688
C G + R + Q WH C
Sbjct: 243 CDGPILRQFVEVYRNGVSQNWHVPC 267
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 31.9 bits (69), Expect = 0.13
Identities = 24/81 (29%), Positives = 33/81 (40%), Gaps = 3/81 (3%)
Frame = +2
Query: 389 YCIKCYESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKL 562
+CIKC+ESV + + + + K WH CF C C L + F
Sbjct: 23 FCIKCWESVPS-------------TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDD 69
Query: 563 DK-IYCGNCYDAQFASRCDGC 622
K I+C C D C+GC
Sbjct: 70 QKQIFCKLCVDI-----CNGC 85
Score = 27.9 bits (59), Expect = 2.1
Identities = 25/102 (24%), Positives = 40/102 (39%), Gaps = 3/102 (2%)
Frame = +2
Query: 302 KDWHSGHFCCWQCDESL--TGQRYVLRDEHP-YCIKCYESVFANGCEECNKIIGIDSKDL 472
K WHS F C C++ L + + + D+ +C C + NGC I +
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD--ICNGCS--TPICEFN---- 93
Query: 473 SYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQ 598
+ + + C C+ CR + FG + YC C Q
Sbjct: 94 AVSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQ 133
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = +2
Query: 611 CDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKS 730
C C E + T ++ + + WH CF CV C + S
Sbjct: 24 CIKCWESVPS-TSQVWFGGKCWHSDCFKCVNCNKKLDPSS 62
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/29 (37%), Positives = 13/29 (44%)
Frame = +2
Query: 317 GHFCCWQCDESLTGQRYVLRDEHPYCIKC 403
GHF C +C L GQ+Y KC
Sbjct: 623 GHFTCQECMSMLVGQKYGSSSTSTIIAKC 651
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 27.5 bits (58), Expect = 2.7
Identities = 23/84 (27%), Positives = 31/84 (36%)
Frame = +2
Query: 476 YKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVFRAGTKKM 655
Y H C++CAKC+ L K G L Y G A R + V K
Sbjct: 4 YYPVHLKSRCYVCAKCKTHLAFK--GHLLSHDYRGKNGPACLFKRVE---NVIEMEPKTE 58
Query: 656 EYKTRQWHEKCFCCVVCKNPIGTK 727
+ T ++ + C C IG K
Sbjct: 59 QMSTGRFIVRHIHCCRCHTYIGWK 82
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 27.1 bits (57), Expect = 3.6
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = -3
Query: 540 STRETRHLAHKKQASCQCLSLYDRSLESMPMILLHSSQPLAKTLS 406
S R T A KQA L+ Y +++M IL + +PLA T S
Sbjct: 753 SPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRS 797
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,487,789
Number of Sequences: 5004
Number of extensions: 42436
Number of successful extensions: 152
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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