Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP23_F_B01
         (847 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po...   115   7e-27
SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharo...    90   4e-19
SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pomb...    44   4e-05
SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase ...    33   0.051
SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo...    31   0.16 
SPAC922.03 |||1-aminocyclopropane-1-carboxylate deaminase |Schiz...    28   1.9  
SPAC12G12.15 |sif3||Sad1 interacting factor 3|Schizosaccharomyce...    27   3.3  
SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharo...    27   4.4  
SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|...    27   4.4  
SPAC23A1.03 |apt1||adenine phosphoribosyltransferase |Schizosacc...    26   7.7  
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe...    26   7.7  

>SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 351

 Score =  115 bits (277), Expect = 7e-27
 Identities = 61/124 (49%), Positives = 78/124 (62%)
 Frame = +3

Query: 414 SDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGGSVKDRIAYRMFLDAEQKGILK 593
           S  +  IG TPL++L+ L  + G  C + AK EF NPGGSVKDR AY +  DAE+KG L 
Sbjct: 15  SGFIGAIGRTPLIRLNTLSNETG--CNILAKAEFQNPGGSVKDRAAYYVVRDAEKKGKLS 72

Query: 594 PGKSVIVEPTSGNTGIGLALAAAVRGYRCIIVLPEXMSDEKVNTXVALGAEIIRTPTEAA 773
            G   IVE T+GNTGIGLA  A  RGY+C+I +P   S  K++T   LGAE+   P  A 
Sbjct: 73  RG-GTIVEGTAGNTGIGLAHIARARGYKCVIYMPNTQSQAKIDTLKFLGAEVHPVPV-AP 130

Query: 774 WDSP 785
           + +P
Sbjct: 131 FSNP 134


>SPAC3A12.17c |cys12|cys1b|cysteine synthase
           Cys12|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 395

 Score = 89.8 bits (213), Expect = 4e-19
 Identities = 56/155 (36%), Positives = 83/155 (53%), Gaps = 6/155 (3%)
 Frame = +3

Query: 315 DAKNGTSNSARKFFTLKDMPHIVRALDRNQKVHSDILN----VIGNTPLVKLSKLPKDEG 482
           D  +G +  A   + L+ +   +R       +  DI+N    +IGNT +V++  L +  G
Sbjct: 7   DLASGIAMGAVFMYLLRRLYESLRVKSSADSLEEDIVNGVEGLIGNTKMVRIKSLSQATG 66

Query: 483 LKCEMYAKCEFLNPGGSVKDRIAYRMFLDAEQKGILKPGKS-VIVEPTSGNTGIGLALAA 659
             C++ AK EFLNPG S KDR+A +M   AE+ G L P +S  + E T+G+TGI +A+  
Sbjct: 67  --CDILAKAEFLNPGNSPKDRVALQMIRTAEENGDLVPYQSNAVYEGTAGSTGISIAMLC 124

Query: 660 AVRGYRCIIVLPEXMSDEKVNTXVALGAEIIR-TP 761
              GY   I +P   S EK +    LGA + R TP
Sbjct: 125 CSLGYDSRIYMPSDQSKEKSDILELLGAHVQRVTP 159


>SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 600

 Score = 43.6 bits (98), Expect = 4e-05
 Identities = 31/113 (27%), Positives = 55/113 (48%)
 Frame = +3

Query: 414 SDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGGSVKDRIAYRMFLDAEQKGILK 593
           S++  VI  TPL K   + +  G+   +Y K E L P  S K R A+      +++ +  
Sbjct: 105 SNVYEVIKETPLTKGVVISESTGVP--VYLKREDLTPVFSFKIRGAHNKMASLDKQSL-- 160

Query: 594 PGKSVIVEPTSGNTGIGLALAAAVRGYRCIIVLPEXMSDEKVNTXVALGAEII 752
             K+ ++  ++GN   G+A +A   G +  IV+P+   + K      LGA ++
Sbjct: 161 --KNGVIACSAGNHAQGVAYSARTLGVKATIVMPQNTPEIKWRNVKRLGANVL 211


>SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 323

 Score = 33.1 bits (72), Expect = 0.051
 Identities = 27/104 (25%), Positives = 49/104 (47%)
 Frame = +3

Query: 441 TPLVKLSKLPKDEGLKCEMYAKCEFLNPGGSVKDRIAYRMFLDAEQKGILKPGKSVIVEP 620
           TP++  S + K+     E++ KCE     G+ K    +R  L+A  +      K+ ++  
Sbjct: 27  TPVLTSSTVNKE--FVAEVFFKCENFQKMGAFK----FRGALNALSQLNEAQRKAGVLTF 80

Query: 621 TSGNTGIGLALAAAVRGYRCIIVLPEXMSDEKVNTXVALGAEII 752
           +SGN    +AL+A + G    I++P    + KV      G ++I
Sbjct: 81  SSGNHAQAIALSAKILGIPAKIIMPLDAPEAKVAATKGYGGQVI 124


>SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein
           Sap49|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 335

 Score = 31.5 bits (68), Expect = 0.16
 Identities = 22/75 (29%), Positives = 36/75 (48%)
 Frame = -1

Query: 805 RNQDVALGESQAASVGVRIISAPNATXVFTFSSDIXSGNTIMHLYPLTAAASASPIPVLP 626
           R+ D+A  +  AA+   ++   P +T    FS    +  +  +     AA S  P+P +P
Sbjct: 184 RHGDIAERKLAAAAKKNKVAVTPQSTLPPGFSPATPAPTSAANTPATIAATSIPPVPNVP 243

Query: 625 DVGSTMTDLPGFKIP 581
            VG+T T +P   IP
Sbjct: 244 LVGAT-TAVPPLSIP 257


>SPAC922.03 |||1-aminocyclopropane-1-carboxylate deaminase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 338

 Score = 27.9 bits (59), Expect = 1.9
 Identities = 13/31 (41%), Positives = 18/31 (58%)
 Frame = +3

Query: 435 GNTPLVKLSKLPKDEGLKCEMYAKCEFLNPG 527
           G TP+  + +L K  G K E++AK E  N G
Sbjct: 14  GPTPITSMKRLSKTLGGKVEIFAKREDCNSG 44


>SPAC12G12.15 |sif3||Sad1 interacting factor 3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 510

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 15/63 (23%), Positives = 32/63 (50%)
 Frame = +3

Query: 291 NSNMSNHVDAKNGTSNSARKFFTLKDMPHIVRALDRNQKVHSDILNVIGNTPLVKLSKLP 470
           N + + H+  +  T+ +++K   L +  H V + +  +  + D+L       L+KL  LP
Sbjct: 77  NRHAAAHIPQR--TTKTSQKLVLLPEN-HAVNSFNEEESNYEDLLTPSDAYNLIKLENLP 133

Query: 471 KDE 479
           +D+
Sbjct: 134 RDK 136


>SPBC31F10.10c |||zf-MYND type zinc finger
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 574

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = +3

Query: 288 INSNMSNHVDAKNGTSNSARKFFTLKDMP 374
           INSN+SN +D+ +  ++SA   F   D+P
Sbjct: 157 INSNISNSLDSVSQHNSSADSLFVNGDVP 185


>SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase
           Rqh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1328

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = +3

Query: 291 NSNMSNHVDAKNGTSNSARKFFT 359
           N+N SN+ +  NGT  +A+ FFT
Sbjct: 321 NNNNSNNNNGNNGTVPNAKTFFT 343


>SPAC23A1.03 |apt1||adenine phosphoribosyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 188

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 15/30 (50%), Positives = 19/30 (63%)
 Frame = +3

Query: 570 AEQKGILKPGKSVIVEPTSGNTGIGLALAA 659
           A QKG +KPG+ V++      TG G ALAA
Sbjct: 110 AIQKGTIKPGQRVLIVDDILATG-GTALAA 138


>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 716

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 14/36 (38%), Positives = 22/36 (61%), Gaps = 2/36 (5%)
 Frame = +3

Query: 456 LSKLPK--DEGLKCEMYAKCEFLNPGGSVKDRIAYR 557
           LS++ K  D GLK  + AK  + NP  +++D + YR
Sbjct: 332 LSEMDKGSDVGLKNCIRAKISYENPNKAMRDPVIYR 367


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,426,166
Number of Sequences: 5004
Number of extensions: 71874
Number of successful extensions: 166
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)