Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP22_F_O02
         (883 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    61   2e-10
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    46   7e-06
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    28   1.5  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    28   2.0  
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ...    27   2.7  
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...    27   3.5  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   6.2  
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo...    26   6.2  
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog...    26   8.2  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 61.3 bits (142), Expect = 2e-10
 Identities = 40/124 (32%), Positives = 67/124 (54%), Gaps = 4/124 (3%)
 Frame = +1

Query: 226 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 393
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+     +L G     +A+V Q+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96

Query: 394 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 573
            S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+LV  R+T
Sbjct: 97  CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156

Query: 574 LADV 585
           LAD+
Sbjct: 157 LADI 160


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 46.0 bits (104), Expect = 7e-06
 Identities = 40/165 (24%), Positives = 74/165 (44%), Gaps = 8/165 (4%)
 Frame = +1

Query: 115 GILYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADG 294
           G LY++  N R    L  A+     V +   +       S D   KFP  K+P F  ADG
Sbjct: 4   GTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIGADG 60

Query: 295 KVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WVFPY 450
             L      + Y+         E L   +   +A + +W  + + +++ P +   WV  +
Sbjct: 61  FELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWVGMF 120

Query: 451 LGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADV 585
            G + + ++  + + +  + +LK+ +  +  RT+LV +R TLAD+
Sbjct: 121 RGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADL 165


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +2

Query: 500 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 592
           TY PP  Y  D+FS APS+   +     S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 20/81 (24%), Positives = 38/81 (46%), Gaps = 1/81 (1%)
 Frame = +1

Query: 79  HSLSXKLQPWRPGILYT-YPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKF 255
           H +S K +  +  +L    P  F++ KA +  QY+          +FG  +K ++F K+ 
Sbjct: 534 HEMSEKFKGCKLILLLEGIPNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEK 593

Query: 256 PAGKVPAFESADGKVLLTESN 318
              ++  F S +  +L+  SN
Sbjct: 594 LESEIVRF-SFEHSILINTSN 613


>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
 Frame = +2

Query: 362 IWLPKPVSGSGHHGLTVNYCL-LPVLGSSLTLV 457
           +W+P PV  SG    T+  C+ LP  G  +T V
Sbjct: 23  LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55


>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 19/80 (23%), Positives = 36/80 (45%), Gaps = 1/80 (1%)
 Frame = +2

Query: 530 DIFSHAPSLLPRESHLPMS-LSSVTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSA 706
           D F  + + + ++S + ++ L+    +H++   L       L NV RWF  + HQ  V  
Sbjct: 129 DEFLKSSTFIAQDSGISVADLAVYARIHSYICGLSAKEGYKLNNVCRWFDFIQHQESVME 188

Query: 707 VVGSLTLCAAPPTYDPKKYQ 766
              S+++  A    +  K Q
Sbjct: 189 AANSMSMKLANIDLNAPKIQ 208


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -3

Query: 302 STFPSALSNAGTFPAGNFFKKSSDL 228
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPBC16A3.11 |eso1||sister chromatid cohesion protein
           Eso1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = +1

Query: 313 SNAIAYYVANESLRGGDLATQARVWQWAS 399
           S + A Y+ NE+LR G +   +R+W  AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808


>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 982

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +2

Query: 410 VNYCLLPVLGSSLTLVSCNSTNRML 484
           V + L+P LG  L + SCN+  R L
Sbjct: 259 VTHALVPELGLQLAMASCNALLRYL 283


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,567,152
Number of Sequences: 5004
Number of extensions: 77024
Number of successful extensions: 254
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)