Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_M22
(904 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 176 3e-45
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 170 3e-43
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 167 1e-42
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 152 8e-38
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 128 1e-30
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 65 1e-11
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 59 1e-09
SPBC23E6.03c |nta1||protein N-terminal amidase Nta1 |Schizosacch... 26 6.4
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 176 bits (429), Expect = 3e-45
Identities = 93/169 (55%), Positives = 114/169 (67%)
Frame = +3
Query: 297 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 476
GTVIGIDLGTTYSCV V KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AAKNQ +N
Sbjct: 35 GTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPSN 93
Query: 477 PENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGRQSICS*RNLCY 656
PENT+FD KRLIGR++ ++T+ D+K FPF +V ++P V+V G++ + +
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVG-GKKKKFTPEEISA 152
Query: 657 GSD*NEXDC*XLPWKKRXXXXXXXCPAYFNDAQRQQPKMPGTISGLNVM 803
K PAYFNDAQRQ K GTI+GLNV+
Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVI 201
Score = 42.7 bits (96), Expect = 7e-05
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +1
Query: 625 KVFAPEEISAMVLTKMXETAXAYLGK 702
K F PEEISAM+L+KM +TA AYLGK
Sbjct: 143 KKFTPEEISAMILSKMKQTAEAYLGK 168
Score = 33.5 bits (73), Expect = 0.042
Identities = 16/28 (57%), Positives = 17/28 (60%)
Frame = +2
Query: 695 LEKKVTHAVVTVPCXFQ*CSTSTTQDAG 778
L K VTHAVVTVP F T+DAG
Sbjct: 166 LGKPVTHAVVTVPAYFNDAQRQATKDAG 193
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 170 bits (413), Expect = 3e-43
Identities = 91/166 (54%), Positives = 110/166 (66%)
Frame = +3
Query: 306 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 485
IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N
Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63
Query: 486 TVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGRQSICS*RNLCYGSD 665
T+FDAKRLIGR ++D VQ D+K +PFKV+EK+ KP +QV+ +G + +
Sbjct: 64 TIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVE-FKGETKTFTPEEISSMVL 122
Query: 666 *NEXDC*XLPWKKRXXXXXXXCPAYFNDAQRQQPKMPGTISGLNVM 803
+ + PAYFND+QRQ K G I+GLNV+
Sbjct: 123 LKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVL 168
Score = 40.3 bits (90), Expect = 4e-04
Identities = 19/48 (39%), Positives = 29/48 (60%)
Frame = +1
Query: 562 PSRSLKRTANLMFKYKLHKGDKVFAPEEISAMVLTKMXETAXAYLGKK 705
P + +++ + + + K F PEEIS+MVL KM E+A A+LG K
Sbjct: 89 PFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGK 136
Score = 29.1 bits (62), Expect = 0.90
Identities = 14/25 (56%), Positives = 15/25 (60%)
Frame = +2
Query: 704 KVTHAVVTVPCXFQ*CSTSTTQDAG 778
KVT AVVTVP F T+DAG
Sbjct: 136 KVTDAVVTVPAYFNDSQRQATKDAG 160
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 167 bits (407), Expect = 1e-42
Identities = 90/166 (54%), Positives = 109/166 (65%)
Frame = +3
Query: 306 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 485
IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N
Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63
Query: 486 TVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGRQSICS*RNLCYGSD 665
T+FDAKRLIGR++ D VQ D+K +PFKV+ K+ KP +QV+ +G + +
Sbjct: 64 TIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVE-YKGETKTFTPEEISSMVL 122
Query: 666 *NEXDC*XLPWKKRXXXXXXXCPAYFNDAQRQQPKMPGTISGLNVM 803
+ + PAYFND+QRQ K G I+GLNV+
Sbjct: 123 MKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVL 168
Score = 44.0 bits (99), Expect = 3e-05
Identities = 22/32 (68%), Positives = 25/32 (78%), Gaps = 1/32 (3%)
Frame = +1
Query: 613 HKGD-KVFAPEEISAMVLTKMXETAXAYLGKK 705
+KG+ K F PEEIS+MVL KM ETA AYLG K
Sbjct: 105 YKGETKTFTPEEISSMVLMKMRETAEAYLGGK 136
Score = 29.1 bits (62), Expect = 0.90
Identities = 14/25 (56%), Positives = 15/25 (60%)
Frame = +2
Query: 704 KVTHAVVTVPCXFQ*CSTSTTQDAG 778
KVT AVVTVP F T+DAG
Sbjct: 136 KVTDAVVTVPAYFNDSQRQATKDAG 160
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 152 bits (368), Expect = 8e-38
Identities = 82/167 (49%), Positives = 102/167 (61%), Gaps = 1/167 (0%)
Frame = +3
Query: 306 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 485
IGIDLGTTYSCV V++ VEII NDQG R TPS+VAFT+ ERL+GDAAKNQ NP N
Sbjct: 9 IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRN 67
Query: 486 TVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQ-TSQGRQSICS*RNLCYGS 662
TVFDAKRLIGR + D Q D+K +PFKV++ N P ++V + +Q + +
Sbjct: 68 TVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLT 127
Query: 663 D*NEXDC*XLPWKKRXXXXXXXCPAYFNDAQRQQPKMPGTISGLNVM 803
E KR PAYF+D+QR K G I+GLNV+
Sbjct: 128 KMKEIS--EAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVL 172
Score = 32.7 bits (71), Expect = 0.073
Identities = 16/27 (59%), Positives = 19/27 (70%)
Frame = +1
Query: 625 KVFAPEEISAMVLTKMXETAXAYLGKK 705
K F +EISAMVLTKM E + A L K+
Sbjct: 114 KQFTAQEISAMVLTKMKEISEAKLNKR 140
Score = 29.1 bits (62), Expect = 0.90
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 695 LEKKVTHAVVTVPCXFQ*CSTSTTQDAG 778
L K+V AV+TVP F + T+DAG
Sbjct: 137 LNKRVEKAVITVPAYFSDSQRAATKDAG 164
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 128 bits (309), Expect = 1e-30
Identities = 76/169 (44%), Positives = 99/169 (58%)
Frame = +3
Query: 297 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 476
G VIGIDLGTT SC+ + + ++IAN +G R TPS VAFT+DGERL+G +AK Q N
Sbjct: 50 GPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVIN 109
Query: 477 PENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGRQSICS*RNLCY 656
PENT F KRLIGR + + VQ D+K P+K+VE +S ++ ++G+ S
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVE-HSNGDAWLE-ARGKTYSPSQIGGFI 167
Query: 657 GSD*NEXDC*XLPWKKRXXXXXXXCPAYFNDAQRQQPKMPGTISGLNVM 803
S E L K PAYFND+QRQ K G I+GLNV+
Sbjct: 168 LSKMRETASTYL--GKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVL 214
Score = 33.9 bits (74), Expect = 0.032
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +1
Query: 625 KVFAPEEISAMVLTKMXETAXAYLGK 702
K ++P +I +L+KM ETA YLGK
Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGK 181
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 64.9 bits (151), Expect = 1e-11
Identities = 35/103 (33%), Positives = 58/103 (56%), Gaps = 1/103 (0%)
Frame = +3
Query: 303 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 482
V+GID G + + + V +N +++I N+ NR TPS V++ + R +G+AAK+ +N
Sbjct: 7 VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSY-GERSRFLGEAAKSAEASNFR 65
Query: 483 NTVFDAKRLIGREWSDQTVQH-DVKFFPFKVVEKNSKPHVQVQ 608
NTV KRL GR + D ++ + F K+ E + +VQ
Sbjct: 66 NTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQ 108
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 58.8 bits (136), Expect = 1e-09
Identities = 28/82 (34%), Positives = 49/82 (59%)
Frame = +3
Query: 300 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 479
TVIGI G S + ++G+ +++AN++GNR PS +++ D E G A+ QL N
Sbjct: 25 TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNA 83
Query: 480 ENTVFDAKRLIGREWSDQTVQH 545
+N+V + + L+G+ + T+ H
Sbjct: 84 DNSVTNFRDLLGKSHDELTLHH 105
>SPBC23E6.03c |nta1||protein N-terminal amidase Nta1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 26.2 bits (55), Expect = 6.4
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 520 NGVTKLYNMMLSSSPSRSLKRTANLMFKYKLHKGDKVFAPE 642
NG++ LYN SP + L N+ K+ L + DK +A E
Sbjct: 97 NGISTLYNSTALISPKKEL---LNVYHKHFLFETDKSWATE 134
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,244,775
Number of Sequences: 5004
Number of extensions: 63275
Number of successful extensions: 184
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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