Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_K19
(870 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 105 6e-24
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 60 4e-10
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 59 7e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 9e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 41 3e-04
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.053
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 31 0.21
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 31 0.28
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 3.5
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 8.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 105 bits (253), Expect = 6e-24
Identities = 52/148 (35%), Positives = 88/148 (59%)
Frame = +2
Query: 128 LPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRL 307
L EQIA R+AF FD ++ G+I + + ++R +GQ L+++I EVDAD +G +
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65
Query: 308 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXX 487
+F EF+T+ A+ + + D E +E+REAF+++DK+GNGYI L +
Sbjct: 66 DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 488 XXXXIQEIDTDGSGTVDFDEFMEMMTGE 571
I+E DTDG G ++++EF +++ +
Sbjct: 123 VADMIREADTDGDGVINYEEFSRVISSK 150
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 60.1 bits (139), Expect = 4e-10
Identities = 38/149 (25%), Positives = 68/149 (45%)
Frame = +2
Query: 125 ELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGR 304
E+ EQ + +AF FD ++ +I + +R +G K + +++ + D G
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89
Query: 305 LEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXX 484
L+ +FV + + IVE D +E++ AF L+D + G I +LR +
Sbjct: 90 LQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQ 146
Query: 485 XXXXXIQEIDTDGSGTVDFDEFMEMMTGE 571
I+E D D G ++ EF+ +M E
Sbjct: 147 ELEAMIEEFDLDQDGEINEQEFIAIMMDE 175
Score = 39.1 bits (87), Expect = 8e-04
Identities = 21/75 (28%), Positives = 40/75 (53%)
Frame = +2
Query: 107 TEDWADELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVD 286
TE + P E+I ++AF+ FD + +G I + + + + + + + LE +IEE D
Sbjct: 100 TEKIVERDPLEEI---KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFD 156
Query: 287 ADKSGRLEFPEFVTL 331
D+ G + EF+ +
Sbjct: 157 LDQDGEINEQEFIAI 171
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 59.3 bits (137), Expect = 7e-10
Identities = 42/149 (28%), Positives = 68/149 (45%), Gaps = 5/149 (3%)
Frame = +2
Query: 137 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 316
E+I +RK F D N+SGSI + I + P + L VD D G ++F
Sbjct: 21 EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGDVDFQ 76
Query: 317 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL-----REIXXXXXXXXXX 481
EF+ + F V + E ++L+ AF++YD + +GYI L +
Sbjct: 77 EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQ 133
Query: 482 XXXXXXIQEIDTDGSGTVDFDEFMEMMTG 568
I E+D D G + F+EF ++++G
Sbjct: 134 QIVDKTIMEVDKDRDGKISFEEFKDIVSG 162
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.6 bits (128), Expect = 9e-09
Identities = 35/140 (25%), Positives = 68/140 (48%)
Frame = +2
Query: 137 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 316
EQ +++AF +D ++ G IP V +LR +G L +L E+ ++
Sbjct: 6 EQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEK 61
Query: 317 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXX 496
+F++ + + E ++E +E +AFR++DK+ +GYI T+ +
Sbjct: 62 KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 497 XIQEIDTDGSGTVDFDEFME 556
+QE D SG+ D+ +F++
Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 40.7 bits (91), Expect = 3e-04
Identities = 30/136 (22%), Positives = 62/136 (45%)
Frame = +2
Query: 155 RKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLA 334
++AF FD + +G IP + D+LR GQ N + E I E+++ ++ +F+ +
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64
Query: 335 AKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEID 514
+ D +E + F+++DK+ G I LR + ++ +
Sbjct: 65 NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVP 123
Query: 515 TDGSGTVDFDEFMEMM 562
G V++ +F++M+
Sbjct: 124 VK-DGMVNYHDFVQMI 138
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 33.1 bits (72), Expect = 0.053
Identities = 21/63 (33%), Positives = 32/63 (50%)
Frame = +2
Query: 263 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 442
E + DADK+G ++F EF+ + V E + +L AF+LYD + NG I +
Sbjct: 66 EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEM 121
Query: 443 REI 451
I
Sbjct: 122 LRI 124
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 31.1 bits (67), Expect = 0.21
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +2
Query: 164 FDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEF 322
++ + +S + V + +M+ + + LEE ++ DAD SG+L F EF
Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 30.7 bits (66), Expect = 0.28
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = -3
Query: 538 VNSAASVSVDLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEFFLHGLS 359
VN A + + DL D AI ++Q+L F +DL + VT+ + +P ++ + +S
Sbjct: 1251 VNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYYTYRVS 1310
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +2
Query: 374 KELREAFRLYDKEGNGYI 427
+EL+EAF L DK+G+G I
Sbjct: 48 QELKEAFALLDKDGDGNI 65
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 25.8 bits (54), Expect = 8.0
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +2
Query: 368 MQKELREAFRLYDKEGNGYIPTSSLR 445
M +E EAF L+D GYI LR
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLR 35
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,698,816
Number of Sequences: 5004
Number of extensions: 47557
Number of successful extensions: 167
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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