Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_J18
(889 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 61 2e-10
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 47 3e-06
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 1.5
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 27 3.6
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 3.6
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 3.6
SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 27 4.7
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 6.2
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 6.2
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 8.2
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 61.3 bits (142), Expect = 2e-10
Identities = 40/124 (32%), Positives = 67/124 (54%), Gaps = 4/124 (3%)
Frame = +3
Query: 186 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 353
N D KFP K+P F DG L+E+ AIA+Y+A+ +L G +A+V Q+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96
Query: 354 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 533
S+++SEL A + P + +++Q + A++ + D L ++T+LV R+T
Sbjct: 97 CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156
Query: 534 LADV 545
LAD+
Sbjct: 157 LADI 160
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 47.2 bits (107), Expect = 3e-06
Identities = 41/168 (24%), Positives = 75/168 (44%), Gaps = 8/168 (4%)
Frame = +3
Query: 66 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 245
M G LY++ N R L A+ V + + S D KFP K+P F
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57
Query: 246 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 401
ADG L + Y+ E L + +A + +W + + +++ P + WV
Sbjct: 58 ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117
Query: 402 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADV 545
+ G + + ++ + + + + +LK+ + + RT+LV +R TLAD+
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADL 165
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 460 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 552
TY PP Y D+FS APS+ + S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 27.1 bits (57), Expect = 3.6
Identities = 19/80 (23%), Positives = 36/80 (45%), Gaps = 1/80 (1%)
Frame = +1
Query: 490 DIFSHAPSLLPRESHLPMS-LSSVTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSA 666
D F + + + ++S + ++ L+ +H++ L L NV RWF + HQ V
Sbjct: 129 DEFLKSSTFIAQDSGISVADLAVYARIHSYICGLSAKEGYKLNNVCRWFDFIQHQESVME 188
Query: 667 VVGSLTLCAAPPTYDPKKYQ 726
S+++ A + K Q
Sbjct: 189 AANSMSMKLANIDLNAPKIQ 208
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 93 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 272
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 273 SN 278
SN
Sbjct: 612 SN 613
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +1
Query: 322 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 417
+W+P PV SG T C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = -2
Query: 144 SRNIVRRSTLYKRGSFPDKCKVPRPPWLEFSERANLKEF 28
SRN TL + S P+ ++ + W+ FSE NL F
Sbjct: 297 SRNYAMNMTLPE--SIPEGYEIDKLDWMSFSESLNLPTF 333
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -1
Query: 262 STFPSALSNAGTFPAGNFFKKSSDL 188
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 273 SNAIAYYVANESLRGGDLATQARVWQWAS 359
S + A Y+ NE+LR G + +R+W AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808
>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = +1
Query: 352 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 459
GH L + C P++GSS L+ +C N R+ V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,460,491
Number of Sequences: 5004
Number of extensions: 72160
Number of successful extensions: 240
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 238
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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