Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_I24
(906 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 252 4e-68
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 239 4e-64
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 239 5e-64
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 206 3e-54
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 178 1e-45
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 99 7e-22
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 91 2e-19
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 60 6e-10
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 252 bits (618), Expect = 4e-68
Identities = 120/162 (74%), Positives = 138/162 (85%)
Frame = +1
Query: 295 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 474
GTVIGIDLGTTYSCV V KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AAKNQ +N
Sbjct: 35 GTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPSN 93
Query: 475 PENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAM 654
PENT+FD KRLIGR++ ++T+ D+K FPF +V ++P V+V K F PEEISAM
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 655 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
+L+KMK+TAEAYLGK VTHAVVTVPAYFNDAQRQATKDAGTI
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTI 195
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/25 (80%), Positives = 24/25 (96%)
Frame = +3
Query: 780 SGLNVMRIINEPTAAAIAYGLDKKE 854
+GLNV+RI+NEPTAAAIAYGLDK +
Sbjct: 196 AGLNVIRIVNEPTAAAIAYGLDKTD 220
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 239 bits (585), Expect = 4e-64
Identities = 117/159 (73%), Positives = 131/159 (82%)
Frame = +1
Query: 304 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 483
IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N
Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63
Query: 484 TVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLT 663
T+FDAKRLIGR++ D VQ D+K +PFKV+ K+ KP +QV+ K F PEEIS+MVL
Sbjct: 64 TIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLM 123
Query: 664 KMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
KM+ETAEAYLG KVT AVVTVPAYFND+QRQATKDAG I
Sbjct: 124 KMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162
Score = 46.0 bits (104), Expect = 7e-06
Identities = 20/23 (86%), Positives = 23/23 (100%)
Frame = +3
Query: 780 SGLNVMRIINEPTAAAIAYGLDK 848
+GLNV+RIINEPTAAAIAYGLD+
Sbjct: 163 AGLNVLRIINEPTAAAIAYGLDR 185
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 239 bits (584), Expect = 5e-64
Identities = 116/159 (72%), Positives = 132/159 (83%)
Frame = +1
Query: 304 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 483
IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N
Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63
Query: 484 TVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLT 663
T+FDAKRLIGR ++D VQ D+K +PFKV+EK+ KP +QV+ K F PEEIS+MVL
Sbjct: 64 TIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLL 123
Query: 664 KMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
KM+E+AEA+LG KVT AVVTVPAYFND+QRQATKDAG I
Sbjct: 124 KMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162
Score = 46.0 bits (104), Expect = 7e-06
Identities = 20/23 (86%), Positives = 23/23 (100%)
Frame = +3
Query: 780 SGLNVMRIINEPTAAAIAYGLDK 848
+GLNV+RIINEPTAAAIAYGLD+
Sbjct: 163 AGLNVLRIINEPTAAAIAYGLDR 185
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 206 bits (503), Expect = 3e-54
Identities = 101/159 (63%), Positives = 119/159 (74%)
Frame = +1
Query: 304 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 483
IGIDLGTTYSCV V++ VEII NDQG R TPS+VAFT+ ERL+GDAAKNQ NP N
Sbjct: 9 IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRN 67
Query: 484 TVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLT 663
TVFDAKRLIGR + D Q D+K +PFKV++ N P ++V K F +EISAMVLT
Sbjct: 68 TVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLT 127
Query: 664 KMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
KMKE +EA L K+V AV+TVPAYF+D+QR ATKDAG I
Sbjct: 128 KMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAI 166
Score = 48.4 bits (110), Expect = 1e-06
Identities = 22/27 (81%), Positives = 24/27 (88%)
Frame = +3
Query: 780 SGLNVMRIINEPTAAAIAYGLDKKEXK 860
+GLNV+RIINEPTAAAIAYGLD K K
Sbjct: 167 AGLNVLRIINEPTAAAIAYGLDAKSDK 193
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 178 bits (433), Expect = 1e-45
Identities = 89/162 (54%), Positives = 116/162 (71%)
Frame = +1
Query: 295 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 474
G VIGIDLGTT SC+ + + ++IAN +G R TPS VAFT+DGERL+G +AK Q N
Sbjct: 50 GPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVIN 109
Query: 475 PENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAM 654
PENT F KRLIGR + + VQ D+K P+K+VE +S ++ ++G K ++P +I
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVE-HSNGDAWLE-ARG-KTYSPSQIGGF 166
Query: 655 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
+L+KM+ETA YLGK V +AVVTVPAYFND+QRQATK AG I
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAI 208
Score = 47.6 bits (108), Expect = 2e-06
Identities = 19/24 (79%), Positives = 24/24 (100%)
Frame = +3
Query: 780 SGLNVMRIINEPTAAAIAYGLDKK 851
+GLNV+R++NEPTAAA+AYGLDKK
Sbjct: 209 AGLNVLRVVNEPTAAALAYGLDKK 232
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 99.1 bits (236), Expect = 7e-22
Identities = 55/161 (34%), Positives = 90/161 (55%), Gaps = 1/161 (0%)
Frame = +1
Query: 301 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 480
V+GID G + + + V +N +++I N+ NR TPS V++ + R +G+AAK+ +N
Sbjct: 7 VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERS-RFLGEAAKSAEASNFR 65
Query: 481 NTVFDAKRLIGREWSDQTVQH-DVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMV 657
NTV KRL GR + D ++ + F K+ E + +VQ + F+ ++ A
Sbjct: 66 NTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAY 125
Query: 658 LTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
TK+K AEA L V+ V+++PA+F D QR+A +A I
Sbjct: 126 FTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANI 166
Score = 30.3 bits (65), Expect = 0.39
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +3
Query: 780 SGLNVMRIINEPTAAAIAYGLDKKE 854
+GLN +R++N+ AAA+ YG+ K +
Sbjct: 167 AGLNPLRLMNDNAAAALTYGITKTD 191
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 91.1 bits (216), Expect = 2e-19
Identities = 55/162 (33%), Positives = 89/162 (54%), Gaps = 4/162 (2%)
Frame = +1
Query: 298 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 477
TVIGI G S + ++G+ +++AN++GNR PS +++ D E G A+ QL N
Sbjct: 25 TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNA 83
Query: 478 ENTVFDAKRLIGREWSDQTVQH-DVKFFPFKVVEK-NSKPHVQV--QTSQGDKVFAPEEI 645
+N+V + + L+G+ + T+ H P V + K VQ ++ +K+ E
Sbjct: 84 DNSVTNFRDLLGKSHDELTLHHCHYSANPVNVEGQIGFKITVQEDEESDPKEKILTAHEA 143
Query: 646 SAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDA 771
S L ++ E+AE +LG KV V++VP YF DAQR+A + A
Sbjct: 144 SVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESA 185
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 59.7 bits (138), Expect = 6e-10
Identities = 47/164 (28%), Positives = 77/164 (46%), Gaps = 3/164 (1%)
Frame = +1
Query: 298 TVIGIDLGTTYSCVGVYKNG-RVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 474
+V+ ID GT ++ + K G +EI+ R S VAF + ER+ G A N T
Sbjct: 23 SVLAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAF-KGNERIFGVDASNLATRF 81
Query: 475 PENTVFDAKRLIGREWSDQT-VQHDVKFFPFKVVEKNSKPHVQVQTSQGDKV-FAPEEIS 648
P +++ + K L+ + VQ +P + +N + + D+ ++ EEI
Sbjct: 82 PAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEII 141
Query: 649 AMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 780
AM + AE +K+T V+TVP +FN+ QR +A I
Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,437,014
Number of Sequences: 5004
Number of extensions: 66828
Number of successful extensions: 211
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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