Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_I23
(944 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 31 0.24
SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces pom... 30 0.55
SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharo... 29 0.95
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 29 1.3
SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imida... 28 2.2
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 27 2.9
SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi... 27 2.9
SPCC417.15 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 5.1
SPAPB2B4.07 |||ubiquitin family protein, human UBTD1 homolog|Sch... 26 6.7
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 26 6.7
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 6.7
SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 26 8.9
SPAC19D5.02c |||peroxisomal membrane protein Pex22 |Schizosaccha... 26 8.9
SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 26 8.9
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 31.1 bits (67), Expect = 0.24
Identities = 25/108 (23%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +1
Query: 388 IYVASNDGIYVYKTGDKSFKKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNKY 567
++ + +DGI++ +F++ F+ + +L + G D T N T G
Sbjct: 379 LFPSGSDGIHLVVDEKSNFREEN-FQRAMSALMEKQGDDPAAMATKGNAKKGKTGKGGVK 437
Query: 568 VLDDNLKAVKQVMFDNFN-VLHYVTLDNEVFKVKETLXKIDFNVKSIR 708
D K VK +M N+N V+ + E + + K+D N ++ R
Sbjct: 438 GPSDIYKIVKMIMVKNYNPVIVFSFSKRECEALALQMSKLDMNDQTER 485
>SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 264
Score = 29.9 bits (64), Expect = 0.55
Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 1/80 (1%)
Frame = +1
Query: 523 NDNKAYKVTENGNKYVLDDNLKAVKQVMF-DNFNVLHYVTLDNEVFKVKETLXKIDFNVK 699
N ++YK E +++ DDN+ K+V + + N TL++ + + L KI NVK
Sbjct: 89 NIEESYKKLEEKTEHLSDDNVSLEKRVEYLETENTKLVKTLNSLNSEFLQLLRKIAINVK 148
Query: 700 SIR*ALEVERTENDAFSFSH 759
R L E + +D S +H
Sbjct: 149 EGR-QLTTENS-SDTSSMTH 166
>SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1205
Score = 29.1 bits (62), Expect = 0.95
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +1
Query: 442 FKKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNK--YVLDDNLKAVKQVMFDN 615
F Y F D + + N +F+ + ++Y V E+GN YVL D + + QV N
Sbjct: 1001 FIAYIRFYVDTV-VNSENVPIVFHLMQRIKQSYDVIEDGNNYIYVLSDMAQKILQVKSQN 1059
Query: 616 F 618
F
Sbjct: 1060 F 1060
>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
Raf2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 28.7 bits (61), Expect = 1.3
Identities = 19/58 (32%), Positives = 27/58 (46%), Gaps = 6/58 (10%)
Frame = -3
Query: 669 LFHFKYFIIQSDI----VQYIEVIKHNL--FDGFQIIIQNVFVTIFSDFVSFIVVCYG 514
LFHF F I S + Y + I H+ F + I + FVT+ DFV + +G
Sbjct: 417 LFHFLLFDIGSGLSGSDYTYEQYINHSAVAFSFTEEIFEKNFVTVLPDFVKLFSISFG 474
>SPBC11B10.02c |his3||histidinol-phosphate aminotransferase
imidazole acetol phosphate transaminase
His3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 256 IYTIHADINDNRIEKGVYEVDLNLN 330
+YT+ A IND + K + E D NLN
Sbjct: 117 MYTVSAKINDVEVVKVLLEPDFNLN 141
>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 27.5 bits (58), Expect = 2.9
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +1
Query: 517 VTNDNKAYKVTENG--NKYVLDDNLKAVKQVMFDNFNVLHYVTLDNEVFKV-KETLXKID 687
V N N +K+T+ N +D+ + + +DN +V LD+E+ + KE K +
Sbjct: 466 VFNSNNKHKMTKQEFINLCDIDELAPEIMEYYYDNITFTPFVNLDDELLLIEKEKNEKYN 525
Query: 688 F 690
F
Sbjct: 526 F 526
>SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 624
Score = 27.5 bits (58), Expect = 2.9
Identities = 11/48 (22%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = -3
Query: 699 FNIKIDFXQSLFHFKYFIIQSDIVQYIEVIKHNLFDGFQII-IQNVFV 559
FN +DF + +F + +++ +EV+K+ L + + ++ + N++V
Sbjct: 472 FNQDLDFDEKMFSRQLSLVKGQAYNIVEVLKNPLMNIYDLLELPNLYV 519
>SPCC417.15 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 43
Score = 26.6 bits (56), Expect = 5.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -2
Query: 424 YIHISRHC*LRISCCHHHNINPFLC 350
+IH S HC C +H INP +C
Sbjct: 18 HIHFSHHC------CENHFINPLVC 36
>SPAPB2B4.07 |||ubiquitin family protein, human UBTD1
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 239
Score = 26.2 bits (55), Expect = 6.7
Identities = 30/134 (22%), Positives = 50/134 (37%), Gaps = 6/134 (4%)
Frame = +1
Query: 178 SVELYSTEDQVVGAFVISNAEKSDGKIYTIHADINDNRIEKGVYEVDLNLNTTVKILDK- 354
S E++ +VV NAE + I +N I KGVY+ KI+ +
Sbjct: 61 SKEIWDVLHKVVTLLYEGNAEAATEMALAADLTIPENDISKGVYDSKGTFYEIPKIVARI 120
Query: 355 -----GRDLCYDDDSTIYVASNDGIYVYKTGDKSFKKYGTFKADVISLTKMNGSDLFYAV 519
R DD+ ++SND + D + K + K + + +
Sbjct: 121 PRAFAERKDSLDDEDDNMISSNDPTKSPEEHDTTTKSIASLKDAELDSSL---ETVLIRY 177
Query: 520 TNDNKAYKVTENGN 561
+ D+K Y + N N
Sbjct: 178 SKDDKDYSIQINPN 191
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 26.2 bits (55), Expect = 6.7
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -1
Query: 320 KSTSYTPFSIRLSLISACIVYILPSLFSALDITKAPT 210
++T S +LS S I + PSL S +TK+P+
Sbjct: 303 RTTEVPSISKQLSKSSTSIATVAPSLASVSSVTKSPS 339
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 26.2 bits (55), Expect = 6.7
Identities = 19/96 (19%), Positives = 40/96 (41%), Gaps = 1/96 (1%)
Frame = +1
Query: 421 YKTGDKSFKKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNKYVLDDNLKAVKQ 600
Y + ++ KA + + N LF + N+NK Y V + + +L + +
Sbjct: 279 YSSPPQTIDPQANIKALITPEDRSNFYQLFSKIDNENKGYIVGGEAVPFFMASHLDSEEL 338
Query: 601 V-MFDNFNVLHYVTLDNEVFKVKETLXKIDFNVKSI 705
++D + +D + F V + K+ + KS+
Sbjct: 339 ARIWDTVDTQDRGYIDKDEFAVAMEIIKLRLSGKSL 374
>SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein
intermediate chain Dic1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 544
Score = 25.8 bits (54), Expect = 8.9
Identities = 20/84 (23%), Positives = 38/84 (45%), Gaps = 3/84 (3%)
Frame = +1
Query: 532 KAYKVTENGNKYVLDDNLK--AVKQVMFDNFNVLHYVTLDNEVFKVKETLXKIDFNVKSI 705
+ Y + + V D LK +V Q FNV ++V ++ ++ET + + K+
Sbjct: 61 QTYSLESKSSNLVSDCELKISSVYQSYSSTFNVSNHVPSISKTVNIRETSKHLKHSTKAP 120
Query: 706 R*ALEVERTENDAF-SFSHTTXGI 774
+ +L E D +F H++ I
Sbjct: 121 KCSLPTSALEIDHLNNFLHSSAKI 144
>SPAC19D5.02c |||peroxisomal membrane protein Pex22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 223
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/76 (21%), Positives = 35/76 (46%)
Frame = +1
Query: 475 ISLTKMNGSDLFYAVTNDNKAYKVTENGNKYVLDDNLKAVKQVMFDNFNVLHYVTLDNEV 654
I + ++ SD ++ +KY +D+ +K + +V D++ +L + NE+
Sbjct: 54 IDWSNLSHSDFVQCGVYEDSTNTWLAGASKYKIDE-IKTLPKVPRDHYIILCDSSESNEI 112
Query: 655 FKVKETLXKIDFNVKS 702
K + + DF+ S
Sbjct: 113 AKFTQVVHSFDFSSDS 128
>SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase
Rdh54|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 25.8 bits (54), Expect = 8.9
Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 8/90 (8%)
Frame = +1
Query: 445 KKYGTFKADVISLTKMNGSDLFYAVTNDNKAYKVTENGNKYV----LDDNLKAVKQVMFD 612
K+ TF+ +I T + S + +++V+EN N++ +D N A V++
Sbjct: 2 KRRATFQCPLIESTIKHQSTNYTKTETATTSHEVSENANEHKGKSNIDINKIAYYNVVWR 61
Query: 613 NFNVLHYVTLDNEVFKVKE----TLXKIDF 690
+ + T + + F V+E TL DF
Sbjct: 62 KITMKKHKTWEGDGFLVREDSNLTLYNSDF 91
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,221,218
Number of Sequences: 5004
Number of extensions: 61903
Number of successful extensions: 191
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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