Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP22_F_I14
         (868 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces...   196   3e-51
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ...    27   3.5  
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2...    27   4.6  
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    26   8.0  
SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharo...    26   8.0  

>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 249

 Score =  196 bits (479), Expect = 3e-51
 Identities = 97/125 (77%), Positives = 105/125 (84%)
 Frame = +2

Query: 77  AVNNISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLG 256
           A   ISKKRKFV DGVF AELNEF TREL+E+GYSG EVRVTP RSEIII AT TQ VLG
Sbjct: 3   AAFTISKKRKFVADGVFYAELNEFFTRELSEEGYSGCEVRVTPSRSEIIIRATHTQDVLG 62

Query: 257 EKGRRIRELTSVVQKRFNIPEQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACY 436
           EKGRRIRELT++VQKRF   E +VELYAEKV  RGLCA+AQ ESLRYKL+ GLAVRRA Y
Sbjct: 63  EKGRRIRELTALVQKRFKFAENTVELYAEKVQNRGLCAVAQCESLRYKLLAGLAVRRAAY 122

Query: 437 GVLRF 451
           GVLR+
Sbjct: 123 GVLRY 127



 Score =  128 bits (308), Expect = 1e-30
 Identities = 60/98 (61%), Positives = 76/98 (77%)
 Frame = +3

Query: 450 FIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTATRHVLLRQGVLGI 629
           ++ME+GA+GCEVV+SGKLR  RAKSMKF DG MIHSG P  D++++ATRHVLLRQGVLG+
Sbjct: 127 YVMEAGAKGCEVVISGKLRAARAKSMKFADGFMIHSGQPAVDFIDSATRHVLLRQGVLGV 186

Query: 630 KVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLEP 743
           KVKIMLP   + K   KK  PD ++V +PK+E    +P
Sbjct: 187 KVKIMLP---EPKTRQKKSLPDIVVVLDPKEEEPITKP 221


>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2111

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 15/50 (30%), Positives = 25/50 (50%)
 Frame = +3

Query: 450  FIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTATRH 599
            F ME   +  + ++  +L+     S K  D ++I  GD  ND + T+ RH
Sbjct: 1870 FAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGTSVRH 1919


>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1217

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
 Frame = +2

Query: 227 MATRTQSVLGEKGRRIRELTSVVQKRFNIPEQSVELYAEKVATRGLCAI-AQAESLRYKL 403
           MATR Q       RR  E  + +QK +N  + ++EL  E+V   G   +  + +  RY +
Sbjct: 726 MATRIQRAWRSYVRRRSEAAACIQKLWNRNKVNMEL--ERVRNEGTKLLQGKKQRRRYSI 783

Query: 404 IG 409
           +G
Sbjct: 784 LG 785


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 2685

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 9/31 (29%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
 Frame = +2

Query: 509  STCQINEVCR-WTHDPLWRPLQ*LRQHCYQT 598
            + C   + CR W   P W+ +  + Q+C+ T
Sbjct: 1420 TVCNRKKACRLWNFKPHWQVITRIPQYCHDT 1450


>SPAC637.11 |suv3||ATP-dependent RNA helicase
           Suv3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 647

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
 Frame = +2

Query: 50  FFSASVAIMAVN-NISKKRKFVGDGVFKAELNEF 148
           FFS S  I  ++ ++ ++RKF G+ ++ A LN F
Sbjct: 16  FFSKSARIHTLSKDVFQQRKFPGNTLWAAALNRF 49


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,134,328
Number of Sequences: 5004
Number of extensions: 62441
Number of successful extensions: 160
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)