Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_I08
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.38
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.50
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.1
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 2.0
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 2.0
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.7
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.6
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.1
SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 23 7.6
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 8.1
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.1
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 30.3 bits (65), Expect = 0.38
Identities = 27/113 (23%), Positives = 47/113 (41%)
Frame = +1
Query: 91 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 270
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 271 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIER 429
SKDA A S+ + S + +L N K++ +L+ ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSR 281
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.50
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +1
Query: 304 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 441
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +1
Query: 283 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 438
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 2.0
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +1
Query: 178 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 348
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 349 KSLQGALGDA 378
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 2.0
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +1
Query: 238 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 399
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 400 LEQSRQNIERTAEE 441
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -3
Query: 415 ASTVPKPPWPCRSRLRALPG 356
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.6
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 184 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 303
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +1
Query: 220 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 378
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 716
Score = 23.0 bits (47), Expect(2) = 7.6
Identities = 8/27 (29%), Positives = 13/27 (48%)
Frame = -2
Query: 173 IAPWARAMQAKRTTNLAAMMYCRETEC 93
+ WA + A N+ + +C TEC
Sbjct: 623 VKEWADFVPALNKKNIVMIPWCNTTEC 649
Score = 21.0 bits (42), Expect(2) = 7.6
Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%)
Frame = -2
Query: 77 PDCNTEECVK-ILRNS 33
P CNT EC K I +NS
Sbjct: 642 PWCNTTECEKEIKKNS 657
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
Frame = +2
Query: 548 KVSSNVQET--NEKLAPKIKAAYD 613
+V N++ET EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 148 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 324
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 325 LQQL 336
Q+
Sbjct: 73 NNQI 76
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,281,093
Number of Sequences: 5004
Number of extensions: 38663
Number of successful extensions: 177
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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