Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_H11
(856 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 77 2e-15
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 72 9e-14
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 71 2e-13
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 50 6e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 46 7e-06
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 39 8e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 38 0.001
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 37 0.003
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 32 0.12
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 77.4 bits (182), Expect = 2e-15
Identities = 38/81 (46%), Positives = 52/81 (64%), Gaps = 2/81 (2%)
Frame = +1
Query: 217 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL--D 390
S+ IEL NF K V ++ F+APWCG+CK LVP Y+K A L ++ V A+ D
Sbjct: 31 SNTIELNSKNFRKFVKAKGPSLVV-FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCD 89
Query: 391 ADEHRSVSQKYGVTGFPTIKI 453
AD++R+V +Y V GFPTIK+
Sbjct: 90 ADQNRAVCSQYQVQGFPTIKL 110
Score = 54.0 bits (124), Expect = 3e-08
Identities = 25/64 (39%), Positives = 36/64 (56%)
Frame = +2
Query: 599 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALECNSSH 778
I L NF++ V LV FYAPWCG+CK L P + K A+ L + + A++C++
Sbjct: 34 IELNSKNFRKFVKAKGPS-LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQ 92
Query: 779 NNGV 790
N V
Sbjct: 93 NRAV 96
Score = 33.1 bits (72), Expect = 0.052
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +3
Query: 753 VPWNATVHTTMASRYQVQGYPTIKLFHQGXKSS 851
V +A + + S+YQVQG+PTIKL + K S
Sbjct: 86 VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGS 118
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 72.1 bits (169), Expect = 9e-14
Identities = 29/83 (34%), Positives = 55/83 (66%), Gaps = 1/83 (1%)
Frame = +1
Query: 211 SSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARAL-KGIVKVGAL 387
+S++V ++ ++L+T +D++ +++F+APWCGHCK+L PEY+ AA L K + + +
Sbjct: 20 ASAEVPKVNKEGLNELIT-ADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEV 78
Query: 388 DADEHRSVSQKYGVTGFPTIKIF 456
D E + +Y + G+PT+ +F
Sbjct: 79 DCTEEGDLCSEYSIRGYPTLNVF 101
Score = 70.1 bits (164), Expect = 4e-13
Identities = 37/102 (36%), Positives = 54/102 (52%), Gaps = 2/102 (1%)
Frame = +1
Query: 208 DSSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVG 381
+S D++ L NFD +V + + ++EF+APWCGHCK+L P Y+K A V V
Sbjct: 352 ESQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVA 411
Query: 382 ALDADEHRSVSQKYGVTGFPTIKIFTGXQAHTISRSEDSRRI 507
+DA E+ +S ++GFPTI F R E R +
Sbjct: 412 KIDATEN-DIS--VSISGFPTIMFFKANDKVNPVRYEGDRTL 450
Score = 59.7 bits (138), Expect = 5e-10
Identities = 25/63 (39%), Positives = 37/63 (58%)
Frame = +2
Query: 596 VITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALECNSS 775
++ L NF ++V+D LVEFYAPWCGHCKNL P + K A E + + +++
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDAT 416
Query: 776 HNN 784
N+
Sbjct: 417 END 419
Score = 51.2 bits (117), Expect = 2e-07
Identities = 21/46 (45%), Positives = 31/46 (67%), Gaps = 1/46 (2%)
Frame = +2
Query: 632 VLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATEL-KGKVKLGALEC 766
++ +D + +V+FYAPWCGHCK L P + AA EL K + L ++C
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDC 80
Score = 29.9 bits (64), Expect = 0.48
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +3
Query: 735 KERSSWVPWNATVHTTMASRYQVQGYPTIKLFHQGXKSS 851
K+ S V + T + S Y ++GYPT+ +F G + S
Sbjct: 70 KDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQIS 108
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 71.3 bits (167), Expect = 2e-13
Identities = 34/81 (41%), Positives = 49/81 (60%), Gaps = 3/81 (3%)
Frame = +1
Query: 214 SSDVIELTPSN-FDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGA 384
+S V+EL N + + S + +IEF+A WCGHCKSL P Y++ + V +G
Sbjct: 19 ASGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGK 78
Query: 385 LDADEHRSVSQKYGVTGFPTI 447
+DAD H V+ KY +TGFPT+
Sbjct: 79 IDADTHSDVADKYHITGFPTL 99
Score = 68.9 bits (161), Expect = 8e-13
Identities = 31/82 (37%), Positives = 49/82 (59%), Gaps = 2/82 (2%)
Frame = +1
Query: 217 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKG--IVKVGALD 390
S+V+EL NFDK+V + + ++EF+A WCG+CK L P Y+ + K V++ ++
Sbjct: 140 SNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKIN 199
Query: 391 ADEHRSVSQKYGVTGFPTIKIF 456
AD + + + V FPTIK F
Sbjct: 200 ADVFADIGRLHEVASFPTIKFF 221
Score = 47.6 bits (108), Expect = 2e-06
Identities = 21/59 (35%), Positives = 32/59 (54%)
Frame = +2
Query: 596 VITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALECNS 772
V+ L NF ++V+D LVEFYA WCG+CK L P + K + + ++ N+
Sbjct: 142 VVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINA 200
Score = 39.5 bits (88), Expect = 6e-04
Identities = 17/41 (41%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Frame = +2
Query: 656 LVEFYAPWCGHCKNLEPHWAK--AATELKGKVKLGALECNS 772
L+EFYA WCGHCK+L P + + A E V +G ++ ++
Sbjct: 43 LIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADT 83
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 49.6 bits (113), Expect = 6e-07
Identities = 22/68 (32%), Positives = 41/68 (60%)
Frame = +1
Query: 265 NSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPT 444
++D++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT
Sbjct: 33 SADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPT 91
Query: 445 IKIFTGXQ 468
+ +F Q
Sbjct: 92 MVLFRKGQ 99
Score = 36.3 bits (80), Expect = 0.006
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 635 LDSDDLWLVEFYAPWCGHCKNLEPHWAKAATE 730
+ +D + +V+FYA WCG CK L+P K + +
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 46.0 bits (104), Expect = 7e-06
Identities = 18/78 (23%), Positives = 44/78 (56%)
Frame = +1
Query: 223 VIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEH 402
V +++ S+ K + D++ +++FFA WCG CK++ P++++ + + +D D+
Sbjct: 2 VKQVSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQL 60
Query: 403 RSVSQKYGVTGFPTIKIF 456
++ + GV P+ ++
Sbjct: 61 SEIAAEAGVHAMPSFFLY 78
Score = 35.5 bits (78), Expect = 0.010
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2
Query: 614 SNFKELVLDSDDLWLVEFYAPWCGHCKNLEP 706
S FK +V D L +V+F+A WCG CK + P
Sbjct: 9 SEFKSIVCQ-DKLVVVDFFATWCGPCKAIAP 38
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 39.1 bits (87), Expect = 8e-04
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +2
Query: 599 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVK 748
+ LTD++ + V S W +++Y P CG CK L P W + K +V+
Sbjct: 29 VPLTDNDLESEV--SKGTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVE 76
Score = 35.5 bits (78), Expect = 0.010
Identities = 11/47 (23%), Positives = 23/47 (48%)
Frame = +2
Query: 626 ELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALEC 766
+ L + W ++FY+ C C ++ W A ++GK+ + + C
Sbjct: 292 DAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINC 338
Score = 35.1 bits (77), Expect = 0.013
Identities = 16/72 (22%), Positives = 34/72 (47%)
Frame = +1
Query: 241 SNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQK 420
++ D +T+ E W I+F++ C C + + A ++G + V ++ + ++
Sbjct: 289 ADIDAALTDK-EGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQ 347
Query: 421 YGVTGFPTIKIF 456
Y + FPT F
Sbjct: 348 YSIQYFPTFLFF 359
Score = 30.3 bits (65), Expect = 0.36
Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 3/80 (3%)
Frame = +1
Query: 226 IELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DAD 396
+ LT ++ + V+ W I+++ P CG CK L P + K V+ + D
Sbjct: 29 VPLTDNDLESEVSKGT--WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 397 EHRSVSQKYGVTGFPTIKIF 456
+ +S + PT+ ++
Sbjct: 87 CSKELSSCANIRAVPTLYLY 106
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +1
Query: 286 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIF 456
++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFF 81
Score = 29.9 bits (64), Expect = 0.48
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2
Query: 659 VEFYAPWCGHCKNLEPHWAKAATE 730
V+ YA WCG CK + P +++ A++
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASK 47
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 37.1 bits (82), Expect = 0.003
Identities = 19/81 (23%), Positives = 41/81 (50%), Gaps = 2/81 (2%)
Frame = +1
Query: 226 IELT-PSNFDKLVTNSDE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADE 399
+E+T F +++ N E I ++ F+APW CK + + + A+ K V + ++A++
Sbjct: 3 VEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEK 61
Query: 400 HRSVSQKYGVTGFPTIKIFTG 462
+++ + V P + G
Sbjct: 62 FSDIAESFDVNAVPLFVLIHG 82
Score = 30.3 bits (65), Expect = 0.36
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +2
Query: 620 FKELVLDS-DDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKL 751
F+E++ + + + L+ FYAPW CK + + + A + K V L
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL 55
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 31.9 bits (69), Expect = 0.12
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +1
Query: 187 TGSLALYDSSSDVIELTPSNFDKLVTNSDEIWIIEFF 297
TGS + SDV+ L+ S L+ SD WII FF
Sbjct: 428 TGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFF 464
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,411,777
Number of Sequences: 5004
Number of extensions: 70941
Number of successful extensions: 235
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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