Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_H04
(871 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 165 7e-42
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 165 1e-41
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 30 0.37
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 30 0.49
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 0.65
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 27 3.5
SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|ch... 27 4.6
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 4.6
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 27 4.6
SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 26 8.0
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 8.0
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 165 bits (401), Expect = 7e-42
Identities = 81/127 (63%), Positives = 100/127 (78%), Gaps = 2/127 (1%)
Frame = +2
Query: 176 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 355
+E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+SWR+I
Sbjct: 5 RENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRASWRII 64
Query: 356 SSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLK 529
SSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKVFY K
Sbjct: 65 SSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYK 124
Query: 530 MKGDYYR 550
MKGDYYR
Sbjct: 125 MKGDYYR 131
Score = 64.9 bits (151), Expect = 1e-11
Identities = 33/56 (58%), Positives = 42/56 (75%), Gaps = 2/56 (3%)
Frame = +1
Query: 691 FSVFYYEILNSPDKACQLAXQAFDDAIAELDTLNEDRTR-FYVNMQLLRDT-DAWT 852
FSVFYYEIL+SP+ AC LA Q FD+AI+ELD+L+E+ + + MQLLRD WT
Sbjct: 178 FSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWT 233
Score = 35.9 bits (79), Expect = 0.007
Identities = 20/53 (37%), Positives = 28/53 (52%)
Frame = +3
Query: 513 KCFTSK*KEITTGYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIR 671
K F K K YLAE GE + S +AY+ A +I+ A++ PT P+R
Sbjct: 119 KVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMR 171
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 165 bits (400), Expect = 1e-41
Identities = 80/127 (62%), Positives = 99/127 (77%), Gaps = 2/127 (1%)
Frame = +2
Query: 176 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 355
+E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SWR++
Sbjct: 6 REDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIV 65
Query: 356 SSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLK 529
SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVFY K
Sbjct: 66 SSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYK 125
Query: 530 MKGDYYR 550
MKGDYYR
Sbjct: 126 MKGDYYR 132
Score = 70.5 bits (165), Expect = 3e-13
Identities = 36/56 (64%), Positives = 44/56 (78%), Gaps = 2/56 (3%)
Frame = +1
Query: 691 FSVFYYEILNSPDKACQLAXQAFDDAIAELDTLNEDRTR-FYVNMQLLRDT-DAWT 852
FSVFYYEILNSPD+AC LA QAFD+AI+ELD+L+E+ + + MQLLRD WT
Sbjct: 179 FSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWT 234
Score = 44.4 bits (100), Expect = 2e-05
Identities = 24/53 (45%), Positives = 30/53 (56%)
Frame = +3
Query: 513 KCFTSK*KEITTGYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIR 671
K F K K YLAE A GE R + S + Y+ A EI+ A++ PTHPIR
Sbjct: 120 KVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIR 172
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 30.3 bits (65), Expect = 0.37
Identities = 21/91 (23%), Positives = 39/91 (42%)
Frame = +2
Query: 179 EELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVIS 358
EEL+ R + + Y+ MA + E E ++ + LLS Y N +R+ +
Sbjct: 3011 EELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDNEAYQAELFYRLSN 3069
Query: 359 SIEQKTEGSERKQQMAKEYRVKVEKELREIC 451
+E+ + EY +E+ L++ C
Sbjct: 3070 CVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 29.9 bits (64), Expect = 0.49
Identities = 19/59 (32%), Positives = 33/59 (55%), Gaps = 3/59 (5%)
Frame = +2
Query: 191 QRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARRSSWRVIS 358
++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V S+W S
Sbjct: 11 EQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREFSTWATFS 68
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 29.5 bits (63), Expect = 0.65
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = +2
Query: 173 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 352
+K+E QR + Q R D M + +G S+ + SV+ + +R S+
Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374
Query: 353 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 442
S E + E SE R+Q++ +E R + EK+LR
Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 27.1 bits (57), Expect = 3.5
Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 1/52 (1%)
Frame = +2
Query: 110 FSPSDK-GISELVLFHRPRCPXDKEELVQRAKLAEQAERYDDMAAAMKEVTE 262
FSP +K + +L LFH + P KE + + + DD A V+E
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSNSNSSDTSTDDQAYLNVSVSE 213
>SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 151
Score = 26.6 bits (56), Expect = 4.6
Identities = 14/53 (26%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = +3
Query: 486 FLKLVIQKVKCF-TSK*KEITTGYLAEVATGETRHSVVEDSQKAYQDAFEISK 641
F+ + V CF K +E+ T + +GE R + D++ YQ I++
Sbjct: 83 FVNKFFRSVGCFGKGKEREVKTDRQRDTGSGEQRIRLERDTETLYQSQLRINQ 135
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 4.6
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 314 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 430
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 4.6
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 3/110 (2%)
Frame = +2
Query: 173 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 343
D ++RA A QA E+ + + +K E+ +L LL V + + R S
Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322
Query: 344 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 493
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 340
Score = 25.8 bits (54), Expect = 8.0
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = -1
Query: 733 PCLANLISHNKRLRKFNARPSLMGCVGCIFALLISK 626
PC +LI+H L + R C C+ A I+K
Sbjct: 36 PCRCSLIAHESCLISYITRSGSTRCPQCLTAYRIAK 71
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 8.0
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 302 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 436
S +Y + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,344,128
Number of Sequences: 5004
Number of extensions: 66774
Number of successful extensions: 202
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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