Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_E20
(946 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 36 0.008
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 36 0.011
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.14
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.24
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.7
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 28 2.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.8
SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 23 4.4
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 26 6.7
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 8.9
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.9 bits (79), Expect = 0.008
Identities = 30/116 (25%), Positives = 33/116 (28%), Gaps = 9/116 (7%)
Frame = -2
Query: 699 SGGXXXXPPPXRXXXPXPPPXXXPXXXXXXXPGGGGGXXXXTXXPPPP-PG-------GX 544
+ G PP R P PPP P P PPP PG
Sbjct: 347 AAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPL 406
Query: 543 GXXSRXXXAPAP-PPRXXXXSPPXRAXGXPXXVRAXXPPXPAXVPXRETXLPXXXG 379
G SR P P PP +PP P + P P P P G
Sbjct: 407 GNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAG 462
Score = 33.5 bits (73), Expect = 0.044
Identities = 30/124 (24%), Positives = 35/124 (28%), Gaps = 7/124 (5%)
Frame = -2
Query: 648 PPPXXXPXXXXXXXPGGGGGXXXXTXXPPPPP------GGXGXXSRXXXAPAPPPRXXXX 487
PPP P P G G + PPPPP G + AP PPP
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Query: 486 SPPXRAXGXPXXVRAXXPP-XPAXVPXRETXLPXXXGXGXXGXXXXXXIXXXTPXPPLAX 310
S + PP P +P R P G + PP A
Sbjct: 371 STGRQPPPLSSSRAVSNPPAPPPAIPGRSA--PALPPLGNASRTSTPPVPTPPSLPPSAP 428
Query: 309 XXXP 298
P
Sbjct: 429 PSLP 432
Score = 28.3 bits (60), Expect = 1.7
Identities = 17/56 (30%), Positives = 18/56 (32%), Gaps = 1/56 (1%)
Frame = -3
Query: 575 PXXPPP-PPXXXXXXPGXXEPPPPXPGXXXKAPPXGXXVXXXXCXPXXPPXPPXYP 411
P PP PP P PP P APP + P P PP P
Sbjct: 425 PSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480
Score = 23.4 bits (48), Expect(2) = 1.8
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +1
Query: 808 PPPPPPXXXPXXXPXPGXG 864
PPPPPP P G G
Sbjct: 312 PPPPPPSRRNRGKPPIGNG 330
Score = 23.0 bits (47), Expect(2) = 1.8
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +1
Query: 805 RPPPPPP 825
RPPPPPP
Sbjct: 310 RPPPPPP 316
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 35.5 bits (78), Expect = 0.011
Identities = 14/30 (46%), Positives = 14/30 (46%)
Frame = -3
Query: 575 PXXPPPPPXXXXXXPGXXEPPPPXPGXXXK 486
P PPPPP P PPPP PG K
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPPGYVKK 35
Score = 26.6 bits (56), Expect = 5.1
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1
Query: 808 PPPPPPXXXPXXXPXP 855
PPPPPP P P P
Sbjct: 11 PPPPPPGFEPPSQPPP 26
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.14
Identities = 19/59 (32%), Positives = 19/59 (32%)
Frame = -2
Query: 678 PPPXRXXXPXPPPXXXPXXXXXXXPGGGGGXXXXTXXPPPPPGGXGXXSRXXXAPAPPP 502
PPP P P P P GG PPPPP G P PPP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGG----------PPPPPPPPGVAGAGPPPPPPPP 782
Score = 31.5 bits (68), Expect = 0.18
Identities = 19/57 (33%), Positives = 19/57 (33%)
Frame = -2
Query: 678 PPPXRXXXPXPPPXXXPXXXXXXXPGGGGGXXXXTXXPPPPPGGXGXXSRXXXAPAP 508
P P P PPP P G PPPPP SR APAP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSR-YYAPAP 797
Score = 31.1 bits (67), Expect = 0.24
Identities = 16/52 (30%), Positives = 16/52 (30%)
Frame = -3
Query: 575 PXXPPPPPXXXXXXPGXXEPPPPXPGXXXKAPPXGXXVXXXXCXPXXPPXPP 420
P PPP P PPP P PP P PP PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.1 bits (67), Expect = 0.24
Identities = 19/61 (31%), Positives = 19/61 (31%)
Frame = +1
Query: 412 GYXGGXGGXXGXHXXXXTXXPXGGAXXXLPGXGGGGSXXPGXXXXXXGGGGGXXGXXXXX 591
G GG GG G GG G GG PG GG GG G
Sbjct: 205 GGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
Query: 592 P 594
P
Sbjct: 265 P 265
Score = 27.9 bits (59), Expect = 2.2
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = -2
Query: 603 GGGGGXXXXTXXPPPPPGGXG 541
GG GG + PPP PGG G
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFG 208
Score = 25.8 bits (54), Expect = 8.9
Identities = 21/79 (26%), Positives = 21/79 (26%)
Frame = +2
Query: 554 GGGGGXXVXXXXPPPPPGXXXXXXXGXXXGGGXGXXXRXGGGXXXXPPEXGXPXXHPXXX 733
GG GG PPP PG G G GGG G P
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGF 247
Query: 734 XXXXXGXXPXXGXGGGXXG 790
G G GG G
Sbjct: 248 GGGLGGFGGGPGGFGGGPG 266
Score = 25.8 bits (54), Expect = 8.9
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +3
Query: 465 PXPGGGGFXXXXGXGGRG 518
P PG GGF G GG G
Sbjct: 200 PPPGPGGFGGFGGFGGEG 217
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.3 bits (60), Expect = 1.7
Identities = 25/107 (23%), Positives = 26/107 (24%), Gaps = 3/107 (2%)
Frame = -3
Query: 788 PXXPPPXPXXGXXPXXXXXXXXGAGXXXPXRXXTXXXPPXPGXXGXXPPXXXXXXGXXXX 609
P PP P P P + PP P PP G
Sbjct: 1136 PSGAPPVPK----PSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPV 1191
Query: 608 XXGGGGXXXXXPXX---PPPPPXXXXXXPGXXEPPPPXPGXXXKAPP 477
P P PPP P PP P KAPP
Sbjct: 1192 PPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238
Score = 27.1 bits (57), Expect = 3.8
Identities = 21/86 (24%), Positives = 24/86 (27%), Gaps = 1/86 (1%)
Frame = -2
Query: 678 PPPXRXXXPXPP-PXXXPXXXXXXXPGGGGGXXXXTXXPPPPPGGXGXXSRXXXAPAPPP 502
PP + PP P P T PP P G S P P P
Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPS--APPPVPAP 1069
Query: 501 RXXXXSPPXRAXGXPXXVRAXXPPXP 424
S P + P + PP P
Sbjct: 1070 SSEIPSIPAPSGAPPVPAPSGIPPVP 1095
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.9 bits (59), Expect = 2.2
Identities = 19/71 (26%), Positives = 21/71 (29%), Gaps = 1/71 (1%)
Frame = -2
Query: 672 PXRXXXPXPPPXXXPXXXXXXX-PGGGGGXXXXTXXPPPPPGGXGXXSRXXXAPAPPPRX 496
P R P P P P + PPPPP S A PPP+
Sbjct: 872 PMRRAAPSMAPVRSPFPGASSAQPAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKV 931
Query: 495 XXXSPPXRAXG 463
P A G
Sbjct: 932 GETYHPPTASG 942
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 3.8
Identities = 15/58 (25%), Positives = 16/58 (27%)
Frame = -3
Query: 596 GGXXXXXPXXPPPPPXXXXXXPGXXEPPPPXPGXXXKAPPXGXXVXXXXCXPXXPPXP 423
GG P PP P P PP P PP + PP P
Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 23.0 bits (47), Expect(2) = 4.4
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = -1
Query: 280 FXXXXXFXXFFFFFFF 233
F F FFFFFFF
Sbjct: 69 FTIEAFFYWFFFFFFF 84
Score = 22.2 bits (45), Expect(2) = 4.4
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -1
Query: 253 FFFFFFFXFF 224
++FFFFF FF
Sbjct: 76 YWFFFFFFFF 85
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 26.2 bits (55), Expect = 6.7
Identities = 13/48 (27%), Positives = 13/48 (27%)
Frame = -3
Query: 566 PPPPPXXXXXXPGXXEPPPPXPGXXXKAPPXGXXVXXXXCXPXXPPXP 423
PPPPP P PP PP P P P
Sbjct: 206 PPPPPPQQNYPPAASSSAPPMQYQQTAYPPQQAPYPPVQAYPQAPQQP 253
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 3/32 (9%)
Frame = -3
Query: 563 PPPPXXXXXXPGXXEPPPP---XPGXXXKAPP 477
PPPP PG PPPP PG PP
Sbjct: 208 PPPPMHHK--PGEHMPPPPMHHEPGEHMPPPP 237
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 3/32 (9%)
Frame = -3
Query: 563 PPPPXXXXXXPGXXEPPPP---XPGXXXKAPP 477
PPPP PG PPPP PG PP
Sbjct: 234 PPPPMHHE--PGEHMPPPPMHHEPGEHMPPPP 263
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 3/32 (9%)
Frame = -3
Query: 563 PPPPXXXXXXPGXXEPPPP---XPGXXXKAPP 477
PPPP PG PPPP PG PP
Sbjct: 260 PPPPMHHE--PGEHMPPPPMHHEPGEHMPPPP 289
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 3/32 (9%)
Frame = -3
Query: 563 PPPPXXXXXXPGXXEPPPP---XPGXXXKAPP 477
PPPP PG PPPP PG PP
Sbjct: 286 PPPPMHHE--PGEHMPPPPMHHEPGEHMPPPP 315
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 3/32 (9%)
Frame = -3
Query: 563 PPPPXXXXXXPGXXEPPPP---XPGXXXKAPP 477
PPPP PG PPPP PG PP
Sbjct: 312 PPPPMHHE--PGEHMPPPPMHHEPGEHMPPPP 341
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,114,349
Number of Sequences: 5004
Number of extensions: 33095
Number of successful extensions: 239
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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