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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP22_F_E04
         (883 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub...    48   2e-06
SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces po...    28   2.0  
SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces pomb...    27   2.7  
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2...    27   4.7  
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p...    26   6.2  
SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c...    26   6.2  
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr...    26   8.2  
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy...    26   8.2  

>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
           subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 44/205 (21%), Positives = 83/205 (40%), Gaps = 4/205 (1%)
 Frame = +2

Query: 236 EPIPIYRVMDN--NGQIID-KNEEPNLDKATLINMYKTMVQLSHMDKILYESQRQGRI-S 403
           +P P+ ++ D+   G  ID  + E  + K  L+ +Y+ MV +  ++       +  +I  
Sbjct: 48  KPFPV-KLDDSVFEGYKIDVPSTEIEVTKGELLGLYEKMVTIRRLELACDALYKAKKIRG 106

Query: 404 FYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTELVNQCYGNCEDPGKGR 583
           F   + G+E +  G   A++  D + + YR  G    RG+++  ++ +  G      KG+
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIITSYRCHGFAYTRGLSIRSIIGELMGRQCGASKGK 166

Query: 584 QMPVHYGSKHHNMVTISSPLGTQMPQAVGAAYAYKRVPE*RPXXXXXXXXXXXXXXXXXX 763
              +H  +K  N    +  +G Q+P   G  +A K + E                     
Sbjct: 167 GGSMHIFAK--NFYGGNGIVGAQIPLGAGIGFAQKYL-EKPTTTFALYGDGASNQGQAFE 223

Query: 764 XFQLCRYVRLSCYMLCRNNGYAIST 838
            F + +   L     C NN Y + T
Sbjct: 224 AFNMAKLWGLPVIFACENNKYGMGT 248


>SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 743

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 15/31 (48%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
 Frame = -2

Query: 366 LSIWLSCTMVLYMLISVALSKF-GSSFLSMI 277
           LSIW S  M+LY L+ V    F GSS +S++
Sbjct: 237 LSIWQSLVMILYYLLYVLFVFFSGSSGVSVV 267


>SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1136

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 13/53 (24%), Positives = 26/53 (49%)
 Frame = +2

Query: 200 VSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSH 358
           +SE+++      + +P Y + D      DK+  PN+D   +  +  T+V L +
Sbjct: 592 LSEIRYSLRELVQDLPSYSLFDTLWVFYDKHIYPNVDPDYISTLIDTLVSLEN 644


>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1217

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
 Frame = +1

Query: 415 QLRRGGHPHRQRLGTLTERFGLQSIQRS-RSVFIPRDDCDGTCEPVLR 555
           ++RR G  +RQ   T  +RF + S + S    +  + D    CE +L+
Sbjct: 641 RIRRAGFAYRQAFDTFAQRFAVLSGKTSYAGEYTWQGDDKSACEQILK 688


>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 926

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = -3

Query: 554 RNTGSQVPSQSSLGIKTLLLLCI 486
           +NTG+ +P Q S  ++ L LLC+
Sbjct: 711 QNTGAAIPLQISTNLRLLPLLCL 733


>SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 547

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 12/45 (26%), Positives = 24/45 (53%)
 Frame = +2

Query: 215 FFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQ 349
           +F  ++Y+ I     +DNN  ++D N  PN + ++  + Y +  Q
Sbjct: 268 YFPNSTYQNI--LNSLDNNPAVLDLNGPPNQESSSSASSYGSRTQ 310


>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 905

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 13/25 (52%), Positives = 16/25 (64%)
 Frame = -1

Query: 577 FPRILAVSVTLVHKFRHSHPSV*KH 503
           FP+  A+SV + HK RH H  V KH
Sbjct: 874 FPKS-ALSVRVKHKRRHRHDRVMKH 897


>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 435

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 11/32 (34%), Positives = 13/32 (40%)
 Frame = -1

Query: 202 HVRCSCARKFCYLTTAILCRFGPRTRRQKPSS 107
           H+ C C   FC+L  A L    P      P S
Sbjct: 383 HMNCLCGTHFCFLCGAYLMEQNPYKHFNDPVS 414


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,526,489
Number of Sequences: 5004
Number of extensions: 72970
Number of successful extensions: 182
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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