Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_E02
(871 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|c... 89 1e-18
SPBC13A2.04c |||PTR family peptide transporter|Schizosaccharomyc... 28 1.5
SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomy... 28 2.0
SPBC8E4.01c ||SPBP4G3.01|inorganic phosphate transporter |Schizo... 27 2.6
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 2.6
SPCC188.09c |||glycoprotein |Schizosaccharomyces pombe|chr 3|||M... 27 3.5
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 26 6.1
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 26 6.1
SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2... 26 6.1
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 26 8.0
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 26 8.0
>SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 433
Score = 88.6 bits (210), Expect = 1e-18
Identities = 50/123 (40%), Positives = 72/123 (58%), Gaps = 1/123 (0%)
Frame = +2
Query: 332 ISTRHLNLQEHHSKDLLRKYQVSIQDFRIIDSKLDTNALS-GFKADEYVVKAQILAGGRG 508
+ R+L L E+ S D+LRK+ V + S + ++ K + VVKAQ+LAGGRG
Sbjct: 18 LQKRNLALHEYISHDILRKFGVDVPRGAPARSGEEAEKVARDLKVTDLVVKAQVLAGGRG 77
Query: 509 KGHFDNGFKGGVHLTKNRDKIVDLAKNMIGNKLITKQTPKEGILVNKVMVAESVNIKRET 688
KG FD+G +GGV + + A+ MIG+KLIT+QT G + N V V E I++E
Sbjct: 78 KGQFDSGLRGGVRPVYDATEARMFAEQMIGHKLITRQTGPAGKICNVVYVCERKFIRKEY 137
Query: 689 YFS 697
YF+
Sbjct: 138 YFA 140
Score = 33.5 bits (73), Expect = 0.040
Identities = 18/63 (28%), Positives = 31/63 (49%)
Frame = +3
Query: 678 KERHTLVIVMERSFNGAAIVASPAGGMDIEAVAEKTPHLVKTVPVDIFEGISDKVANEIA 857
++ + I+M+R IVAS GG+DIE VA + P + + + +A E+
Sbjct: 134 RKEYYFAILMDRENQCPMIVASDQGGVDIETVAAENPSAIIKRSLPNSPNLDPHIAEELV 193
Query: 858 EFL 866
+ L
Sbjct: 194 DKL 196
>SPBC13A2.04c |||PTR family peptide transporter|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 618
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = -1
Query: 574 DNLV-PIFCKMNSPFKPIVKVT 512
DN++ P+ K N PFKPI+++T
Sbjct: 413 DNIIYPLLRKYNIPFKPILRIT 434
>SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 573
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +2
Query: 467 EYVVKAQILAGGRGKGHFDNGFKGGVHLTKN 559
EY+ I G GKGH+ +G +G V+ N
Sbjct: 73 EYLYWGGIEKGPNGKGHYPSGIRGLVNAASN 103
>SPBC8E4.01c ||SPBP4G3.01|inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 572
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 467 EYVVKAQILAGGRGKGHFDNGFKGGVHLTKN 559
EY+ I G GKGH+ +G +G V+ + N
Sbjct: 73 EYLYWGGIEKGPTGKGHYPSGIRGLVNASAN 103
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 27.5 bits (58), Expect = 2.6
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +3
Query: 648 KLWSQKV*ISKERHTLVIVMERSFNGAAIVASPAGGMDIEAVAEKTPHLVKTVPVDIFEG 827
KLW +K+ ++K+RH+ I ++ F +V+ A + L + + EG
Sbjct: 844 KLWVEKINVAKKRHSQKINIKNPF-ALKVVSDVAFQYPPSDLVNGNEPLNSFNEITLVEG 902
Query: 828 IS-DKVANEIA 857
S D+ NE+A
Sbjct: 903 SSIDRALNEVA 913
>SPCC188.09c |||glycoprotein |Schizosaccharomyces pombe|chr
3|||Manual
Length = 609
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +2
Query: 443 ALSGFKADEYVVKAQILAGGRGKGHFDNGF 532
++ AD YV ++A G GKG FD F
Sbjct: 545 SMGSLTADTYVPVRFVVANGAGKGGFDFSF 574
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +2
Query: 587 NMIGNKLITKQTPKEGILVNKVMVAESVNIKRETYFSYCNGE 712
N + L+ Q +E + N V ESVN+ TY+ G+
Sbjct: 158 NKVDRALLELQISQEELYQNFARVVESVNVVISTYYDKVLGD 199
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +2
Query: 587 NMIGNKLITKQTPKEGILVNKVMVAESVNIKRETYFSYCNGE 712
N + L+ Q +E + N V ESVN+ TY+ G+
Sbjct: 158 NKVDRALLELQISQEELYQNFARVVESVNVVISTYYDKVLGD 199
>SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr
2|||Manual
Length = 803
Score = 26.2 bits (55), Expect = 6.1
Identities = 19/59 (32%), Positives = 28/59 (47%)
Frame = +2
Query: 335 STRHLNLQEHHSKDLLRKYQVSIQDFRIIDSKLDTNALSGFKADEYVVKAQILAGGRGK 511
++ LN +E + +D L VSI + +LDTN L+ D V AQ+ GK
Sbjct: 467 NSEFLNNEEENDEDELSNVLVSIPSRTSVLERLDTNPLNE-STDAVVGCAQLYYPSLGK 524
>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1116
Score = 25.8 bits (54), Expect = 8.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 341 RHLNLQEHHSKDLLRKYQVSIQDFRIIDSKLDTNALSG 454
RH N + + S +LLR+ + RI++ K DT SG
Sbjct: 778 RH-NARRNKSGNLLRRSSTKLWGSRIVELKPDTTITSG 814
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 25.8 bits (54), Expect = 8.0
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = -2
Query: 324 GQYVDEARFMSLKLFKFLKAAIILF*LNLNHETGSLTELNLDNFH 190
G + +E + L + F A I F L L+H + + ELN FH
Sbjct: 201 GDHEEETVHIHLTISLFASAIISGFALLLDHPSAHIRELNSRFFH 245
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,138,166
Number of Sequences: 5004
Number of extensions: 61461
Number of successful extensions: 152
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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