Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP22_F_D23
         (934 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    30   0.54 
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    29   1.2  
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz...    29   1.2  
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    28   2.2  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    27   2.9  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   3.8  
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation...    26   6.6  
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce...    26   8.7  

>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 168

 Score = 29.9 bits (64), Expect = 0.54
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +3

Query: 306 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 443
           KDG+++   +LN FAK L  +    + +A +   +    IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156


>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 20/78 (25%), Positives = 37/78 (47%)
 Frame = +3

Query: 198 FFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGD 377
           F + +EH+     K+LE+Q + L +SKDA     A      S+     +   S + +L  
Sbjct: 206 FMEPVEHN-HPLSKSLEEQSSFLEQSKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLAS 264

Query: 378 ANGKAKEALEQSRQNIER 431
            N K++ +L+    ++ R
Sbjct: 265 LN-KSRASLQHKIMSLSR 281


>SPBC646.09c |int6|yin6|translation initiation factor
           eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
 Frame = +3

Query: 285 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 440
           +D + A+ +   SVLQ+L+   + +QG LG   N     AL Q + QN++   E
Sbjct: 79  EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 27.9 bits (59), Expect = 2.2
 Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
 Frame = +3

Query: 240 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 401
           T++ +  SL K  D     +  ++  ES L  L      L  A      L D+  KAK  
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411

Query: 402 LEQSRQNIERTAEE 443
           LEQ+R  +ERT  E
Sbjct: 412 LEQARIELERTQRE 425


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 27.5 bits (58), Expect = 2.9
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -2

Query: 417 ASTVPKPPWPCRSRLRALPG 358
           AST+ K PWP  + L  +PG
Sbjct: 38  ASTLEKEPWPASTALLVMPG 57


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 27.1 bits (57), Expect = 3.8
 Identities = 12/41 (29%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
 Frame = +3

Query: 189 LPSFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 305
           + S+F D++ H K FH   E+  +   +  +K   D  K W
Sbjct: 666 MKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706


>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
           factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 321

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +3

Query: 222 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 380
           +K+   + E+Q   +T      DFS   K   +     L+AF K L+  + GDA
Sbjct: 89  SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142


>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 512

 Score = 25.8 bits (54), Expect = 8.7
 Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
 Frame = +1

Query: 550 KVSSNVQET--NEKLAPKIKAAYD 615
           +V  N++ET   EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,291,745
Number of Sequences: 5004
Number of extensions: 37763
Number of successful extensions: 173
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)